Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03400 and RR42_RS03405 overlap by 8 nucleotides RR42_RS03405 and RR42_RS03410 are separated by 16 nucleotides RR42_RS03410 and RR42_RS03415 overlap by 7 nucleotides
RR42_RS03400: RR42_RS03400 - permease, at 756,703 to 757,596
_RS03400
RR42_RS03405: RR42_RS03405 - epoxyqueuosine reductase, at 757,589 to 758,809
_RS03405
RR42_RS03410: RR42_RS03410 - ATPase, at 758,826 to 759,338
_RS03410
RR42_RS03415: RR42_RS03415 - N-acetylmuramoyl-L-alanine amidase, at 759,332 to 760,837
_RS03415
Position (kb) 757
758
759 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 756.611 kb on + strand at 756.612 kb on - strand at 756.612 kb on - strand at 756.653 kb on - strand at 756.688 kb on - strand at 756.688 kb on - strand at 756.688 kb on - strand at 756.691 kb on + strand at 756.818 kb on - strand, within RR42_RS03400 at 756.940 kb on + strand, within RR42_RS03400 at 756.941 kb on - strand, within RR42_RS03400 at 757.018 kb on + strand, within RR42_RS03400 at 757.018 kb on + strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.483 kb on + strand, within RR42_RS03400 at 757.483 kb on + strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.485 kb on + strand, within RR42_RS03400 at 757.486 kb on - strand, within RR42_RS03400 at 757.486 kb on - strand, within RR42_RS03400 at 757.625 kb on - strand at 757.947 kb on + strand, within RR42_RS03405 at 757.947 kb on + strand, within RR42_RS03405 at 757.947 kb on + strand, within RR42_RS03405 at 757.948 kb on - strand, within RR42_RS03405 at 758.013 kb on + strand, within RR42_RS03405 at 758.349 kb on + strand, within RR42_RS03405 at 758.452 kb on - strand, within RR42_RS03405 at 758.533 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.820 kb on + strand at 758.820 kb on + strand at 758.820 kb on + strand at 759.506 kb on + strand, within RR42_RS03415 at 759.506 kb on + strand, within RR42_RS03415 at 759.507 kb on - strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.745 kb on + strand, within RR42_RS03415 at 759.746 kb on - strand, within RR42_RS03415 at 759.794 kb on + strand, within RR42_RS03415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 756,611 + +0.7 756,612 - +0.6 756,612 - -0.4 756,653 - -1.1 756,688 - +1.0 756,688 - +0.8 756,688 - +0.8 756,691 + +0.7 756,818 - RR42_RS03400 0.13 -0.0 756,940 + RR42_RS03400 0.27 -1.2 756,941 - RR42_RS03400 0.27 -3.6 757,018 + RR42_RS03400 0.35 -2.0 757,018 + RR42_RS03400 0.35 -1.2 757,019 - RR42_RS03400 0.35 -1.7 757,019 - RR42_RS03400 0.35 -1.0 757,019 - RR42_RS03400 0.35 +0.3 757,483 + RR42_RS03400 0.87 +0.7 757,483 + RR42_RS03400 0.87 +1.4 757,484 - RR42_RS03400 0.87 +1.1 757,484 - RR42_RS03400 0.87 +1.1 757,484 - RR42_RS03400 0.87 -0.6 757,484 - RR42_RS03400 0.87 +0.2 757,485 + RR42_RS03400 0.87 +0.4 757,486 - RR42_RS03400 0.88 +0.1 757,486 - RR42_RS03400 0.88 +0.2 757,625 - -2.2 757,947 + RR42_RS03405 0.29 +1.0 757,947 + RR42_RS03405 0.29 +1.3 757,947 + RR42_RS03405 0.29 +0.2 757,948 - RR42_RS03405 0.29 +0.5 758,013 + RR42_RS03405 0.35 +1.6 758,349 + RR42_RS03405 0.62 +1.5 758,452 - RR42_RS03405 0.71 -0.2 758,533 + RR42_RS03405 0.77 -0.4 758,535 + RR42_RS03405 0.77 -0.3 758,535 + RR42_RS03405 0.77 -0.7 758,535 + RR42_RS03405 0.77 -1.2 758,535 + RR42_RS03405 0.77 -2.0 758,535 + RR42_RS03405 0.77 +1.6 758,820 + -0.2 758,820 + -0.2 758,820 + +1.5 759,506 + RR42_RS03415 0.12 +2.6 759,506 + RR42_RS03415 0.12 +2.2 759,507 - RR42_RS03415 0.12 -2.2 759,656 + RR42_RS03415 0.22 +0.0 759,656 + RR42_RS03415 0.22 +2.8 759,657 - RR42_RS03415 0.22 +1.0 759,657 - RR42_RS03415 0.22 +0.3 759,745 + RR42_RS03415 0.27 +2.8 759,746 - RR42_RS03415 0.27 +1.5 759,794 + RR42_RS03415 0.31 +0.2
Or see this region's nucleotide sequence