Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS03280

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS03270 and RR42_RS03275 are separated by 94 nucleotidesRR42_RS03275 and RR42_RS03280 are separated by 117 nucleotidesRR42_RS03280 and RR42_RS03285 are separated by 57 nucleotides RR42_RS03270: RR42_RS03270 - membrane protein, at 724,868 to 725,077 _RS03270 RR42_RS03275: RR42_RS03275 - membrane protein, at 725,172 to 725,642 _RS03275 RR42_RS03280: RR42_RS03280 - LysR family transcriptional regulator, at 725,760 to 726,665 _RS03280 RR42_RS03285: RR42_RS03285 - tricarboxylic transport membrane protein, at 726,723 to 727,679 _RS03285 Position (kb) 725 726 727Strain fitness (log2 ratio) -3 -2 -1 0 1at 724.764 kb on + strandat 724.764 kb on + strandat 724.765 kb on - strandat 724.765 kb on - strandat 724.765 kb on - strandat 724.765 kb on - strandat 724.874 kb on - strandat 724.879 kb on - strandat 724.888 kb on + strandat 724.888 kb on + strandat 725.166 kb on + strandat 725.166 kb on + strandat 725.167 kb on - strandat 725.167 kb on - strandat 725.167 kb on - strandat 725.167 kb on - strandat 725.187 kb on + strandat 725.281 kb on + strand, within RR42_RS03275at 725.290 kb on + strand, within RR42_RS03275at 725.290 kb on + strand, within RR42_RS03275at 725.290 kb on + strand, within RR42_RS03275at 725.290 kb on + strand, within RR42_RS03275at 725.290 kb on + strand, within RR42_RS03275at 725.290 kb on + strand, within RR42_RS03275at 725.291 kb on - strand, within RR42_RS03275at 725.291 kb on - strand, within RR42_RS03275at 725.291 kb on - strand, within RR42_RS03275at 725.479 kb on + strand, within RR42_RS03275at 725.480 kb on - strand, within RR42_RS03275at 725.612 kb on + strandat 725.613 kb on - strandat 725.667 kb on + strandat 725.667 kb on + strandat 725.667 kb on + strandat 725.667 kb on + strandat 725.668 kb on - strandat 725.700 kb on - strandat 726.067 kb on - strand, within RR42_RS03280at 726.194 kb on - strand, within RR42_RS03280at 726.194 kb on - strand, within RR42_RS03280at 726.194 kb on - strand, within RR42_RS03280at 726.194 kb on - strand, within RR42_RS03280at 726.663 kb on + strandat 726.664 kb on - strandat 726.698 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.784 kb on + strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.785 kb on - strandat 726.788 kb on + strandat 726.788 kb on + strandat 726.898 kb on + strand, within RR42_RS03285at 726.899 kb on - strand, within RR42_RS03285at 726.899 kb on - strand, within RR42_RS03285at 726.928 kb on + strand, within RR42_RS03285at 726.929 kb on - strand, within RR42_RS03285at 726.929 kb on - strand, within RR42_RS03285at 727.165 kb on + strand, within RR42_RS03285at 727.165 kb on + strand, within RR42_RS03285at 727.166 kb on - strand, within RR42_RS03285at 727.245 kb on + strand, within RR42_RS03285at 727.245 kb on + strand, within RR42_RS03285at 727.245 kb on + strand, within RR42_RS03285at 727.245 kb on + strand, within RR42_RS03285at 727.246 kb on - strand, within RR42_RS03285at 727.246 kb on - strand, within RR42_RS03285at 727.246 kb on - strand, within RR42_RS03285at 727.320 kb on + strand, within RR42_RS03285at 727.331 kb on - strand, within RR42_RS03285at 727.375 kb on + strand, within RR42_RS03285at 727.376 kb on - strand, within RR42_RS03285at 727.376 kb on - strand, within RR42_RS03285at 727.474 kb on + strand, within RR42_RS03285at 727.474 kb on + strand, within RR42_RS03285at 727.474 kb on + strand, within RR42_RS03285at 727.474 kb on + strand, within RR42_RS03285at 727.475 kb on - strand, within RR42_RS03285at 727.505 kb on - strand, within RR42_RS03285

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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724,764 + -0.4
724,764 + -2.2
724,765 - -0.5
724,765 - +0.5
724,765 - +0.3
724,765 - -0.7
724,874 - +0.2
724,879 - +1.0
724,888 + -1.1
724,888 + -1.1
725,166 + -0.6
725,166 + +0.0
725,167 - +0.6
725,167 - -2.7
725,167 - -0.5
725,167 - -1.4
725,187 + -0.4
725,281 + RR42_RS03275 0.23 +1.4
725,290 + RR42_RS03275 0.25 +0.8
725,290 + RR42_RS03275 0.25 -0.8
725,290 + RR42_RS03275 0.25 +0.6
725,290 + RR42_RS03275 0.25 +1.0
725,290 + RR42_RS03275 0.25 -2.8
725,290 + RR42_RS03275 0.25 +0.8
725,291 - RR42_RS03275 0.25 -0.8
725,291 - RR42_RS03275 0.25 +0.8
725,291 - RR42_RS03275 0.25 -0.3
725,479 + RR42_RS03275 0.65 -1.2
725,480 - RR42_RS03275 0.65 -0.3
725,612 + -0.2
725,613 - -0.4
725,667 + +1.7
725,667 + +1.2
725,667 + +0.1
725,667 + -1.8
725,668 - +1.1
725,700 - +0.2
726,067 - RR42_RS03280 0.34 +0.2
726,194 - RR42_RS03280 0.48 +0.5
726,194 - RR42_RS03280 0.48 +0.1
726,194 - RR42_RS03280 0.48 +0.2
726,194 - RR42_RS03280 0.48 -0.8
726,663 + -0.3
726,664 - +0.6
726,698 + -0.4
726,784 + -0.8
726,784 + +0.4
726,784 + -1.5
726,784 + -0.9
726,784 + -0.5
726,784 + -1.2
726,784 + +0.4
726,784 + -2.2
726,784 + -0.6
726,784 + -0.2
726,784 + -1.2
726,785 - +1.5
726,785 - -0.3
726,785 - -1.7
726,785 - -0.1
726,785 - +0.6
726,785 - -0.6
726,785 - -1.1
726,785 - -0.6
726,785 - +1.2
726,785 - +1.0
726,788 + +1.0
726,788 + -0.1
726,898 + RR42_RS03285 0.18 +0.6
726,899 - RR42_RS03285 0.18 +1.2
726,899 - RR42_RS03285 0.18 -0.2
726,928 + RR42_RS03285 0.21 -0.8
726,929 - RR42_RS03285 0.22 +0.4
726,929 - RR42_RS03285 0.22 +0.5
727,165 + RR42_RS03285 0.46 -0.2
727,165 + RR42_RS03285 0.46 +1.0
727,166 - RR42_RS03285 0.46 -0.4
727,245 + RR42_RS03285 0.55 +0.6
727,245 + RR42_RS03285 0.55 +0.7
727,245 + RR42_RS03285 0.55 +1.1
727,245 + RR42_RS03285 0.55 -0.5
727,246 - RR42_RS03285 0.55 -1.1
727,246 - RR42_RS03285 0.55 +0.1
727,246 - RR42_RS03285 0.55 -0.2
727,320 + RR42_RS03285 0.62 +1.5
727,331 - RR42_RS03285 0.64 -0.7
727,375 + RR42_RS03285 0.68 -1.0
727,376 - RR42_RS03285 0.68 -2.5
727,376 - RR42_RS03285 0.68 +0.4
727,474 + RR42_RS03285 0.78 +0.1
727,474 + RR42_RS03285 0.78 -0.6
727,474 + RR42_RS03285 0.78 -1.2
727,474 + RR42_RS03285 0.78 +0.8
727,475 - RR42_RS03285 0.79 -2.8
727,505 - RR42_RS03285 0.82 -2.0

Or see this region's nucleotide sequence