Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03250 and RR42_RS03255 are separated by 39 nucleotides RR42_RS03255 and RR42_RS03260 overlap by 4 nucleotides RR42_RS03260 and RR42_RS03265 are separated by 161 nucleotides
RR42_RS03250: RR42_RS03250 - MarR family transcriptional regulator, at 720,856 to 721,416
_RS03250
RR42_RS03255: RR42_RS03255 - hypothetical protein, at 721,456 to 721,956
_RS03255
RR42_RS03260: RR42_RS03260 - branched-chain amino acid ABC transporter substrate-binding protein, at 721,953 to 723,071
_RS03260
RR42_RS03265: RR42_RS03265 - hypothetical protein, at 723,233 to 724,513
_RS03265
Position (kb) 721
722
723
724 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 721.007 kb on + strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.221 kb on + strand, within RR42_RS03250 at 721.457 kb on + strand at 721.458 kb on - strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.460 kb on - strand at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.671 kb on - strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 722.038 kb on + strand at 722.039 kb on - strand at 722.067 kb on - strand, within RR42_RS03260 at 722.067 kb on - strand, within RR42_RS03260 at 722.180 kb on - strand, within RR42_RS03260 at 722.180 kb on - strand, within RR42_RS03260 at 722.219 kb on - strand, within RR42_RS03260 at 722.242 kb on + strand, within RR42_RS03260 at 722.384 kb on - strand, within RR42_RS03260 at 722.528 kb on - strand, within RR42_RS03260 at 722.704 kb on + strand, within RR42_RS03260 at 722.705 kb on - strand, within RR42_RS03260 at 722.906 kb on + strand, within RR42_RS03260 at 722.907 kb on - strand, within RR42_RS03260 at 722.907 kb on - strand, within RR42_RS03260 at 722.907 kb on - strand, within RR42_RS03260 at 722.908 kb on + strand, within RR42_RS03260 at 723.195 kb on - strand at 723.195 kb on - strand at 723.234 kb on + strand at 723.285 kb on + strand at 723.286 kb on - strand at 723.463 kb on - strand, within RR42_RS03265 at 723.519 kb on + strand, within RR42_RS03265 at 723.520 kb on - strand, within RR42_RS03265 at 723.520 kb on - strand, within RR42_RS03265 at 723.599 kb on + strand, within RR42_RS03265 at 723.654 kb on + strand, within RR42_RS03265 at 723.654 kb on + strand, within RR42_RS03265 at 723.655 kb on - strand, within RR42_RS03265 at 723.655 kb on - strand, within RR42_RS03265 at 723.918 kb on + strand, within RR42_RS03265 at 723.918 kb on + strand, within RR42_RS03265 at 723.919 kb on - strand, within RR42_RS03265 at 723.988 kb on - strand, within RR42_RS03265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 721,007 + RR42_RS03250 0.27 +0.8 721,218 - RR42_RS03250 0.65 +0.1 721,218 - RR42_RS03250 0.65 -0.6 721,221 + RR42_RS03250 0.65 -0.9 721,457 + +1.0 721,458 - -0.7 721,459 + -3.3 721,459 + +0.1 721,459 + +0.3 721,459 + +1.0 721,459 + +0.9 721,460 - +1.2 721,583 + RR42_RS03255 0.25 -1.2 721,583 + RR42_RS03255 0.25 -0.6 721,583 + RR42_RS03255 0.25 +0.8 721,670 + RR42_RS03255 0.43 +0.9 721,670 + RR42_RS03255 0.43 +1.3 721,670 + RR42_RS03255 0.43 +1.5 721,670 + RR42_RS03255 0.43 -0.5 721,671 - RR42_RS03255 0.43 +0.2 721,703 + RR42_RS03255 0.49 -1.1 721,703 + RR42_RS03255 0.49 -1.4 722,038 + -0.0 722,039 - -1.4 722,067 - RR42_RS03260 0.10 +0.1 722,067 - RR42_RS03260 0.10 -0.7 722,180 - RR42_RS03260 0.20 -1.7 722,180 - RR42_RS03260 0.20 -1.2 722,219 - RR42_RS03260 0.24 +1.1 722,242 + RR42_RS03260 0.26 -1.0 722,384 - RR42_RS03260 0.39 -1.7 722,528 - RR42_RS03260 0.51 -1.4 722,704 + RR42_RS03260 0.67 -0.9 722,705 - RR42_RS03260 0.67 +0.5 722,906 + RR42_RS03260 0.85 -0.8 722,907 - RR42_RS03260 0.85 -0.5 722,907 - RR42_RS03260 0.85 +0.6 722,907 - RR42_RS03260 0.85 -0.1 722,908 + RR42_RS03260 0.85 +0.2 723,195 - +0.3 723,195 - +0.8 723,234 + +2.8 723,285 + +0.1 723,286 - +1.2 723,463 - RR42_RS03265 0.18 +1.0 723,519 + RR42_RS03265 0.22 -1.7 723,520 - RR42_RS03265 0.22 +0.4 723,520 - RR42_RS03265 0.22 -1.4 723,599 + RR42_RS03265 0.29 -0.8 723,654 + RR42_RS03265 0.33 -2.5 723,654 + RR42_RS03265 0.33 +0.5 723,655 - RR42_RS03265 0.33 +0.4 723,655 - RR42_RS03265 0.33 -1.0 723,918 + RR42_RS03265 0.53 +0.5 723,918 + RR42_RS03265 0.53 +2.8 723,919 - RR42_RS03265 0.54 +0.2 723,988 - RR42_RS03265 0.59 +1.0
Or see this region's nucleotide sequence