Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02925 and RR42_RS02930 are separated by 163 nucleotides RR42_RS02930 and RR42_RS02935 are separated by 9 nucleotides RR42_RS02935 and RR42_RS02940 overlap by 4 nucleotides RR42_RS02940 and RR42_RS02945 are separated by 24 nucleotides
RR42_RS02925: RR42_RS02925 - membrane protein, at 652,034 to 652,945
_RS02925
RR42_RS02930: RR42_RS02930 - AsnC family transcriptional regulator, at 653,109 to 653,579
_RS02930
RR42_RS02935: RR42_RS02935 - malonate decarboxylase subunit epsilon, at 653,589 to 654,527
_RS02935
RR42_RS02940: RR42_RS02940 - phosphoribosyl-dephospho-CoA transferase, at 654,524 to 655,162
_RS02940
RR42_RS02945: RR42_RS02945 - malonate decarboxylase subunit gamma, at 655,187 to 656,005
_RS02945
Position (kb) 653
654
655 Strain fitness (log2 ratio) -2
-1
0
1
2 at 652.668 kb on + strand, within RR42_RS02925 at 652.668 kb on + strand, within RR42_RS02925 at 652.669 kb on - strand, within RR42_RS02925 at 652.669 kb on - strand, within RR42_RS02925 at 653.022 kb on - strand at 653.410 kb on - strand, within RR42_RS02930 at 653.553 kb on + strand at 653.853 kb on - strand, within RR42_RS02935 at 653.863 kb on + strand, within RR42_RS02935 at 653.864 kb on - strand, within RR42_RS02935 at 654.160 kb on + strand, within RR42_RS02935 at 654.172 kb on + strand, within RR42_RS02935 at 654.435 kb on + strand at 654.516 kb on + strand at 654.516 kb on + strand at 654.517 kb on - strand at 654.517 kb on - strand at 654.517 kb on - strand at 654.942 kb on - strand, within RR42_RS02940 at 655.401 kb on + strand, within RR42_RS02945 at 655.401 kb on + strand, within RR42_RS02945 at 655.416 kb on + strand, within RR42_RS02945 at 655.417 kb on - strand, within RR42_RS02945 at 655.417 kb on - strand, within RR42_RS02945 at 655.508 kb on + strand, within RR42_RS02945 at 655.509 kb on - strand, within RR42_RS02945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 652,668 + RR42_RS02925 0.70 +0.2 652,668 + RR42_RS02925 0.70 -1.2 652,669 - RR42_RS02925 0.70 -1.4 652,669 - RR42_RS02925 0.70 +0.3 653,022 - +0.6 653,410 - RR42_RS02930 0.64 -0.1 653,553 + -0.7 653,853 - RR42_RS02935 0.28 -0.2 653,863 + RR42_RS02935 0.29 -0.6 653,864 - RR42_RS02935 0.29 -0.9 654,160 + RR42_RS02935 0.61 -2.5 654,172 + RR42_RS02935 0.62 +0.1 654,435 + -1.1 654,516 + +1.8 654,516 + +0.1 654,517 - -0.6 654,517 - -0.8 654,517 - -1.9 654,942 - RR42_RS02940 0.65 -0.9 655,401 + RR42_RS02945 0.26 +1.0 655,401 + RR42_RS02945 0.26 -2.1 655,416 + RR42_RS02945 0.28 -0.4 655,417 - RR42_RS02945 0.28 -0.6 655,417 - RR42_RS02945 0.28 -1.4 655,508 + RR42_RS02945 0.39 -0.9 655,509 - RR42_RS02945 0.39 +0.2
Or see this region's nucleotide sequence