Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02720 and RR42_RS02725 are separated by 17 nucleotides RR42_RS02725 and RR42_RS02730 overlap by 4 nucleotides RR42_RS02730 and RR42_RS02735 are separated by 240 nucleotides
RR42_RS02720: RR42_RS02720 - phosphoribosylaminoimidazolecarboxamide formyltransferase, at 606,073 to 607,647
_RS02720
RR42_RS02725: RR42_RS02725 - Fis family transcriptional regulator, at 607,665 to 607,898
_RS02725
RR42_RS02730: RR42_RS02730 - tRNA-dihydrouridine synthase B, at 607,895 to 608,989
_RS02730
RR42_RS02735: RR42_RS02735 - hypothetical protein, at 609,230 to 610,477
_RS02735
Position (kb) 607
608
609 Strain fitness (log2 ratio) -2
-1
0
1
2 at 607.652 kb on - strand at 607.919 kb on - strand at 608.119 kb on - strand, within RR42_RS02730 at 608.167 kb on - strand, within RR42_RS02730 at 608.167 kb on - strand, within RR42_RS02730 at 608.176 kb on - strand, within RR42_RS02730 at 608.257 kb on - strand, within RR42_RS02730 at 608.428 kb on - strand, within RR42_RS02730 at 608.446 kb on - strand, within RR42_RS02730 at 609.013 kb on - strand at 609.013 kb on - strand at 609.050 kb on - strand at 609.050 kb on - strand at 609.166 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 607,652 - -0.8 607,919 - +2.7 608,119 - RR42_RS02730 0.20 -0.1 608,167 - RR42_RS02730 0.25 -0.7 608,167 - RR42_RS02730 0.25 -0.5 608,176 - RR42_RS02730 0.26 -1.9 608,257 - RR42_RS02730 0.33 +0.8 608,428 - RR42_RS02730 0.49 -0.5 608,446 - RR42_RS02730 0.50 -1.8 609,013 - +0.1 609,013 - +1.1 609,050 - +0.4 609,050 - -0.3 609,166 + +0.9
Or see this region's nucleotide sequence