Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02555 and RR42_RS02560 are separated by 60 nucleotides RR42_RS02560 and RR42_RS02565 are separated by 174 nucleotides RR42_RS02565 and RR42_RS02570 are separated by 177 nucleotides
RR42_RS02555: RR42_RS02555 - transcriptional regulator, at 575,043 to 575,501
_RS02555
RR42_RS02560: RR42_RS02560 - cysteine dioxygenase, at 575,562 to 576,158
_RS02560
RR42_RS02565: RR42_RS02565 - LysR family transcriptional regulator, at 576,333 to 577,304
_RS02565
RR42_RS02570: RR42_RS02570 - glutamate dehydrogenase, at 577,482 to 578,789
_RS02570
Position (kb) 576
577
578 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 575.362 kb on - strand, within RR42_RS02555 at 575.620 kb on + strand at 575.621 kb on - strand at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.624 kb on + strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.625 kb on - strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.898 kb on + strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.899 kb on - strand, within RR42_RS02560 at 575.914 kb on + strand, within RR42_RS02560 at 575.914 kb on + strand, within RR42_RS02560 at 575.915 kb on - strand, within RR42_RS02560 at 576.189 kb on + strand at 576.190 kb on - strand at 576.246 kb on + strand at 576.412 kb on + strand at 576.412 kb on + strand at 576.413 kb on - strand at 576.413 kb on - strand at 576.526 kb on - strand, within RR42_RS02565 at 576.526 kb on - strand, within RR42_RS02565 at 576.772 kb on - strand, within RR42_RS02565 at 576.772 kb on - strand, within RR42_RS02565 at 576.792 kb on - strand, within RR42_RS02565 at 576.813 kb on + strand, within RR42_RS02565 at 576.852 kb on + strand, within RR42_RS02565 at 576.852 kb on + strand, within RR42_RS02565 at 576.886 kb on - strand, within RR42_RS02565 at 576.930 kb on + strand, within RR42_RS02565 at 576.930 kb on + strand, within RR42_RS02565 at 576.930 kb on + strand, within RR42_RS02565 at 576.931 kb on - strand, within RR42_RS02565 at 576.931 kb on - strand, within RR42_RS02565 at 576.932 kb on + strand, within RR42_RS02565 at 576.932 kb on + strand, within RR42_RS02565 at 576.933 kb on - strand, within RR42_RS02565 at 577.012 kb on - strand, within RR42_RS02565 at 577.340 kb on + strand at 577.341 kb on - strand at 577.341 kb on - strand at 577.572 kb on + strand at 577.573 kb on - strand at 577.846 kb on - strand, within RR42_RS02570 at 577.846 kb on - strand, within RR42_RS02570 at 577.865 kb on + strand, within RR42_RS02570 at 577.923 kb on + strand, within RR42_RS02570 at 577.924 kb on - strand, within RR42_RS02570 at 577.924 kb on - strand, within RR42_RS02570 at 577.924 kb on - strand, within RR42_RS02570 at 577.924 kb on - strand, within RR42_RS02570 at 577.925 kb on + strand, within RR42_RS02570 at 577.925 kb on + strand, within RR42_RS02570 at 577.926 kb on - strand, within RR42_RS02570 at 578.022 kb on + strand, within RR42_RS02570 at 578.022 kb on + strand, within RR42_RS02570 at 578.023 kb on - strand, within RR42_RS02570 at 578.023 kb on - strand, within RR42_RS02570 at 578.023 kb on - strand, within RR42_RS02570 at 578.283 kb on + strand, within RR42_RS02570 at 578.283 kb on + strand, within RR42_RS02570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 575,362 - RR42_RS02555 0.69 -1.9 575,620 + -1.3 575,621 - -0.7 575,624 + RR42_RS02560 0.10 -2.1 575,624 + RR42_RS02560 0.10 -1.1 575,624 + RR42_RS02560 0.10 +0.3 575,624 + RR42_RS02560 0.10 -3.5 575,625 - RR42_RS02560 0.11 +1.3 575,625 - RR42_RS02560 0.11 -1.3 575,625 - RR42_RS02560 0.11 +0.6 575,898 + RR42_RS02560 0.56 -1.8 575,898 + RR42_RS02560 0.56 +1.4 575,898 + RR42_RS02560 0.56 -0.1 575,899 - RR42_RS02560 0.56 +0.5 575,899 - RR42_RS02560 0.56 +0.4 575,899 - RR42_RS02560 0.56 +3.2 575,914 + RR42_RS02560 0.59 +1.2 575,914 + RR42_RS02560 0.59 +0.8 575,915 - RR42_RS02560 0.59 +0.1 576,189 + +0.1 576,190 - +1.4 576,246 + +0.4 576,412 + +1.1 576,412 + -0.0 576,413 - -0.2 576,413 - -1.6 576,526 - RR42_RS02565 0.20 +1.2 576,526 - RR42_RS02565 0.20 -0.4 576,772 - RR42_RS02565 0.45 -1.9 576,772 - RR42_RS02565 0.45 -0.8 576,792 - RR42_RS02565 0.47 -1.5 576,813 + RR42_RS02565 0.49 +0.2 576,852 + RR42_RS02565 0.53 -3.8 576,852 + RR42_RS02565 0.53 -2.1 576,886 - RR42_RS02565 0.57 -1.6 576,930 + RR42_RS02565 0.61 -0.6 576,930 + RR42_RS02565 0.61 -1.0 576,930 + RR42_RS02565 0.61 +0.1 576,931 - RR42_RS02565 0.62 +0.7 576,931 - RR42_RS02565 0.62 -0.3 576,932 + RR42_RS02565 0.62 -2.5 576,932 + RR42_RS02565 0.62 +1.2 576,933 - RR42_RS02565 0.62 -2.1 577,012 - RR42_RS02565 0.70 +0.2 577,340 + -0.0 577,341 - -0.8 577,341 - -0.3 577,572 + -3.1 577,573 - -1.7 577,846 - RR42_RS02570 0.28 -1.8 577,846 - RR42_RS02570 0.28 +0.0 577,865 + RR42_RS02570 0.29 -1.3 577,923 + RR42_RS02570 0.34 -0.2 577,924 - RR42_RS02570 0.34 -0.8 577,924 - RR42_RS02570 0.34 -1.8 577,924 - RR42_RS02570 0.34 -1.8 577,924 - RR42_RS02570 0.34 +1.0 577,925 + RR42_RS02570 0.34 -0.1 577,925 + RR42_RS02570 0.34 +1.1 577,926 - RR42_RS02570 0.34 -0.9 578,022 + RR42_RS02570 0.41 +0.0 578,022 + RR42_RS02570 0.41 -2.8 578,023 - RR42_RS02570 0.41 +0.7 578,023 - RR42_RS02570 0.41 +0.4 578,023 - RR42_RS02570 0.41 -1.6 578,283 + RR42_RS02570 0.61 -0.4 578,283 + RR42_RS02570 0.61 +1.6
Or see this region's nucleotide sequence