Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02480 and RR42_RS02485 are separated by 135 nucleotides RR42_RS02485 and RR42_RS02490 overlap by 16 nucleotides RR42_RS02490 and RR42_RS02495 are separated by 141 nucleotides
RR42_RS02480: RR42_RS02480 - LysR family transcriptional regulator, at 557,250 to 558,134
_RS02480
RR42_RS02485: RR42_RS02485 - short-chain dehydrogenase, at 558,270 to 558,869
_RS02485
RR42_RS02490: RR42_RS02490 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 558,854 to 559,411
_RS02490
RR42_RS02495: RR42_RS02495 - cardiolipin synthase; exported protein, at 559,553 to 560,872
_RS02495
Position (kb) 558
559
560 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 558.036 kb on + strand, within RR42_RS02480 at 558.036 kb on + strand, within RR42_RS02480 at 558.036 kb on + strand, within RR42_RS02480 at 558.036 kb on + strand, within RR42_RS02480 at 558.037 kb on - strand, within RR42_RS02480 at 558.037 kb on - strand, within RR42_RS02480 at 558.142 kb on + strand at 558.153 kb on + strand at 558.278 kb on + strand at 558.278 kb on + strand at 558.279 kb on - strand at 558.530 kb on - strand, within RR42_RS02485 at 558.530 kb on - strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.737 kb on - strand, within RR42_RS02485 at 558.760 kb on - strand, within RR42_RS02485 at 558.855 kb on + strand at 558.976 kb on + strand, within RR42_RS02490 at 559.101 kb on + strand, within RR42_RS02490 at 559.101 kb on + strand, within RR42_RS02490 at 559.102 kb on - strand, within RR42_RS02490 at 559.449 kb on - strand at 559.449 kb on - strand at 559.790 kb on + strand, within RR42_RS02495 at 559.790 kb on + strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.797 kb on - strand, within RR42_RS02495 at 559.825 kb on - strand, within RR42_RS02495 at 559.953 kb on + strand, within RR42_RS02495 at 559.953 kb on + strand, within RR42_RS02495 at 560.190 kb on + strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 558,036 + RR42_RS02480 0.89 -0.6 558,036 + RR42_RS02480 0.89 +1.7 558,036 + RR42_RS02480 0.89 -0.1 558,036 + RR42_RS02480 0.89 +0.7 558,037 - RR42_RS02480 0.89 -0.7 558,037 - RR42_RS02480 0.89 -1.2 558,142 + -1.7 558,153 + -0.8 558,278 + +0.9 558,278 + +2.2 558,279 - +0.7 558,530 - RR42_RS02485 0.43 -0.9 558,530 - RR42_RS02485 0.43 +0.2 558,736 + RR42_RS02485 0.78 +0.8 558,736 + RR42_RS02485 0.78 +0.0 558,736 + RR42_RS02485 0.78 +0.3 558,737 - RR42_RS02485 0.78 -0.4 558,760 - RR42_RS02485 0.82 -0.8 558,855 + +0.5 558,976 + RR42_RS02490 0.22 -2.3 559,101 + RR42_RS02490 0.44 -1.3 559,101 + RR42_RS02490 0.44 -0.8 559,102 - RR42_RS02490 0.44 -3.1 559,449 - -1.8 559,449 - +1.0 559,790 + RR42_RS02495 0.18 +0.7 559,790 + RR42_RS02495 0.18 +0.2 559,791 - RR42_RS02495 0.18 +0.6 559,791 - RR42_RS02495 0.18 +1.7 559,791 - RR42_RS02495 0.18 +0.3 559,797 - RR42_RS02495 0.18 +1.2 559,825 - RR42_RS02495 0.21 -0.8 559,953 + RR42_RS02495 0.30 -0.2 559,953 + RR42_RS02495 0.30 +0.4 560,190 + RR42_RS02495 0.48 +1.0 560,406 - RR42_RS02495 0.65 -0.1 560,406 - RR42_RS02495 0.65 +2.4 560,406 - RR42_RS02495 0.65 -0.2
Or see this region's nucleotide sequence