Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02465 and RR42_RS02470 are separated by 105 nucleotides RR42_RS02470 and RR42_RS02475 overlap by 4 nucleotides RR42_RS02475 and RR42_RS02480 are separated by 21 nucleotides
RR42_RS02465: RR42_RS02465 - membrane protein, at 554,977 to 555,864
_RS02465
RR42_RS02470: RR42_RS02470 - dihydroneopterin triphosphate pyrophosphatase, at 555,970 to 556,443
_RS02470
RR42_RS02475: RR42_RS02475 - endonuclease, at 556,440 to 557,228
_RS02475
RR42_RS02480: RR42_RS02480 - LysR family transcriptional regulator, at 557,250 to 558,134
_RS02480
Position (kb) 555
556
557 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 554.990 kb on + strand at 554.991 kb on - strand at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.434 kb on - strand, within RR42_RS02465 at 555.434 kb on - strand, within RR42_RS02465 at 555.516 kb on + strand, within RR42_RS02465 at 555.516 kb on + strand, within RR42_RS02465 at 555.598 kb on + strand, within RR42_RS02465 at 555.623 kb on - strand, within RR42_RS02465 at 555.623 kb on - strand, within RR42_RS02465 at 555.939 kb on + strand at 555.964 kb on - strand at 555.964 kb on - strand at 555.976 kb on + strand at 556.204 kb on + strand, within RR42_RS02470 at 556.204 kb on + strand, within RR42_RS02470 at 556.322 kb on - strand, within RR42_RS02470 at 556.322 kb on - strand, within RR42_RS02470 at 556.439 kb on + strand at 556.439 kb on + strand at 556.461 kb on + strand at 556.462 kb on - strand at 556.632 kb on + strand, within RR42_RS02475 at 556.632 kb on + strand, within RR42_RS02475 at 556.633 kb on - strand, within RR42_RS02475 at 556.684 kb on - strand, within RR42_RS02475 at 556.684 kb on - strand, within RR42_RS02475 at 556.802 kb on + strand, within RR42_RS02475 at 557.074 kb on - strand, within RR42_RS02475 at 557.074 kb on - strand, within RR42_RS02475 at 557.074 kb on - strand, within RR42_RS02475 at 557.074 kb on - strand, within RR42_RS02475 at 557.074 kb on - strand, within RR42_RS02475 at 557.391 kb on + strand, within RR42_RS02480 at 557.392 kb on - strand, within RR42_RS02480 at 557.392 kb on - strand, within RR42_RS02480
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 554,990 + +0.2 554,991 - -1.9 555,433 + RR42_RS02465 0.51 +1.4 555,433 + RR42_RS02465 0.51 -1.5 555,433 + RR42_RS02465 0.51 -0.2 555,433 + RR42_RS02465 0.51 -1.3 555,433 + RR42_RS02465 0.51 -3.0 555,434 - RR42_RS02465 0.51 -0.5 555,434 - RR42_RS02465 0.51 -2.8 555,516 + RR42_RS02465 0.61 -0.0 555,516 + RR42_RS02465 0.61 -0.3 555,598 + RR42_RS02465 0.70 -0.5 555,623 - RR42_RS02465 0.73 +1.0 555,623 - RR42_RS02465 0.73 -0.2 555,939 + +0.5 555,964 - -0.3 555,964 - -0.1 555,976 + +0.5 556,204 + RR42_RS02470 0.49 -1.0 556,204 + RR42_RS02470 0.49 -2.3 556,322 - RR42_RS02470 0.74 +0.0 556,322 - RR42_RS02470 0.74 -1.1 556,439 + +0.2 556,439 + +0.7 556,461 + -0.8 556,462 - -2.7 556,632 + RR42_RS02475 0.24 -1.1 556,632 + RR42_RS02475 0.24 -1.6 556,633 - RR42_RS02475 0.24 -1.9 556,684 - RR42_RS02475 0.31 -1.3 556,684 - RR42_RS02475 0.31 -1.3 556,802 + RR42_RS02475 0.46 -2.0 557,074 - RR42_RS02475 0.80 -1.9 557,074 - RR42_RS02475 0.80 -0.8 557,074 - RR42_RS02475 0.80 -0.3 557,074 - RR42_RS02475 0.80 -0.3 557,074 - RR42_RS02475 0.80 -0.5 557,391 + RR42_RS02480 0.16 -0.2 557,392 - RR42_RS02480 0.16 -1.6 557,392 - RR42_RS02480 0.16 -1.7
Or see this region's nucleotide sequence