Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02305 and RR42_RS02310 are separated by 78 nucleotides RR42_RS02310 and RR42_RS02315 are separated by 15 nucleotides
RR42_RS02305: RR42_RS02305 - porin, at 516,087 to 517,421
_RS02305
RR42_RS02310: RR42_RS02310 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 517,500 to 518,333
_RS02310
RR42_RS02315: RR42_RS02315 - threonine dehydratase, at 518,349 to 519,875
_RS02315
Position (kb) 517
518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 516.618 kb on + strand, within RR42_RS02305 at 516.618 kb on + strand, within RR42_RS02305 at 516.702 kb on + strand, within RR42_RS02305 at 516.726 kb on + strand, within RR42_RS02305 at 516.726 kb on + strand, within RR42_RS02305 at 516.726 kb on + strand, within RR42_RS02305 at 516.820 kb on - strand, within RR42_RS02305 at 516.850 kb on - strand, within RR42_RS02305 at 516.850 kb on - strand, within RR42_RS02305 at 517.062 kb on - strand, within RR42_RS02305 at 517.062 kb on - strand, within RR42_RS02305 at 517.086 kb on + strand, within RR42_RS02305 at 517.087 kb on - strand, within RR42_RS02305 at 517.190 kb on + strand, within RR42_RS02305 at 517.191 kb on - strand, within RR42_RS02305 at 517.191 kb on - strand, within RR42_RS02305 at 517.215 kb on + strand, within RR42_RS02305 at 517.215 kb on + strand, within RR42_RS02305 at 517.215 kb on + strand, within RR42_RS02305 at 517.216 kb on - strand, within RR42_RS02305 at 517.266 kb on + strand, within RR42_RS02305 at 517.266 kb on + strand, within RR42_RS02305 at 517.267 kb on - strand, within RR42_RS02305 at 517.267 kb on - strand, within RR42_RS02305 at 517.296 kb on + strand at 517.296 kb on + strand at 517.317 kb on + strand at 517.317 kb on + strand at 517.344 kb on + strand at 517.344 kb on + strand at 517.445 kb on + strand at 517.587 kb on - strand, within RR42_RS02310 at 517.700 kb on - strand, within RR42_RS02310 at 517.705 kb on - strand, within RR42_RS02310 at 517.870 kb on + strand, within RR42_RS02310 at 517.870 kb on + strand, within RR42_RS02310 at 517.871 kb on - strand, within RR42_RS02310 at 518.369 kb on - strand at 518.369 kb on - strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.398 kb on - strand at 518.398 kb on - strand at 518.402 kb on - strand at 518.402 kb on - strand at 518.516 kb on + strand, within RR42_RS02315 at 518.587 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.757 kb on - strand, within RR42_RS02315 at 518.785 kb on + strand, within RR42_RS02315 at 518.995 kb on + strand, within RR42_RS02315 at 519.264 kb on + strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.296 kb on - strand, within RR42_RS02315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 516,618 + RR42_RS02305 0.40 +1.1 516,618 + RR42_RS02305 0.40 +2.6 516,702 + RR42_RS02305 0.46 +0.6 516,726 + RR42_RS02305 0.48 -0.8 516,726 + RR42_RS02305 0.48 -0.4 516,726 + RR42_RS02305 0.48 -1.2 516,820 - RR42_RS02305 0.55 -0.4 516,850 - RR42_RS02305 0.57 -0.3 516,850 - RR42_RS02305 0.57 +0.4 517,062 - RR42_RS02305 0.73 +1.1 517,062 - RR42_RS02305 0.73 +1.4 517,086 + RR42_RS02305 0.75 +1.4 517,087 - RR42_RS02305 0.75 -0.1 517,190 + RR42_RS02305 0.83 -0.9 517,191 - RR42_RS02305 0.83 -0.3 517,191 - RR42_RS02305 0.83 +1.8 517,215 + RR42_RS02305 0.84 -1.0 517,215 + RR42_RS02305 0.84 -3.1 517,215 + RR42_RS02305 0.84 -1.5 517,216 - RR42_RS02305 0.85 +1.2 517,266 + RR42_RS02305 0.88 +1.4 517,266 + RR42_RS02305 0.88 -0.1 517,267 - RR42_RS02305 0.88 -0.0 517,267 - RR42_RS02305 0.88 +0.2 517,296 + +1.1 517,296 + -1.3 517,317 + +1.0 517,317 + -1.7 517,344 + +0.3 517,344 + +1.1 517,445 + -1.1 517,587 - RR42_RS02310 0.10 -0.8 517,700 - RR42_RS02310 0.24 -0.3 517,705 - RR42_RS02310 0.25 +0.9 517,870 + RR42_RS02310 0.44 -0.6 517,870 + RR42_RS02310 0.44 +0.6 517,871 - RR42_RS02310 0.44 +1.3 518,369 - -2.5 518,369 - -1.4 518,397 + +1.2 518,397 + +0.8 518,397 + +0.3 518,398 - -0.4 518,398 - -0.4 518,402 - -1.2 518,402 - -0.0 518,516 + RR42_RS02315 0.11 -1.3 518,587 + RR42_RS02315 0.16 -0.5 518,632 + RR42_RS02315 0.19 -0.4 518,632 + RR42_RS02315 0.19 +1.0 518,632 + RR42_RS02315 0.19 -0.8 518,757 - RR42_RS02315 0.27 -1.9 518,785 + RR42_RS02315 0.29 +1.5 518,995 + RR42_RS02315 0.42 +0.4 519,264 + RR42_RS02315 0.60 +1.2 519,265 - RR42_RS02315 0.60 -3.7 519,265 - RR42_RS02315 0.60 +0.8 519,296 - RR42_RS02315 0.62 -0.3
Or see this region's nucleotide sequence