Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS02305

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS02300 and RR42_RS02305 are separated by 284 nucleotidesRR42_RS02305 and RR42_RS02310 are separated by 78 nucleotidesRR42_RS02310 and RR42_RS02315 are separated by 15 nucleotides RR42_RS02300: RR42_RS02300 - hypothetical protein, at 515,131 to 515,802 _RS02300 RR42_RS02305: RR42_RS02305 - porin, at 516,087 to 517,421 _RS02305 RR42_RS02310: RR42_RS02310 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 517,500 to 518,333 _RS02310 RR42_RS02315: RR42_RS02315 - threonine dehydratase, at 518,349 to 519,875 _RS02315 Position (kb) 516 517 518Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 515.232 kb on + strand, within RR42_RS02300at 515.232 kb on + strand, within RR42_RS02300at 515.232 kb on + strand, within RR42_RS02300at 515.232 kb on + strand, within RR42_RS02300at 515.233 kb on - strand, within RR42_RS02300at 515.233 kb on - strand, within RR42_RS02300at 515.233 kb on - strand, within RR42_RS02300at 515.233 kb on - strand, within RR42_RS02300at 515.241 kb on + strand, within RR42_RS02300at 515.606 kb on + strand, within RR42_RS02300at 515.607 kb on - strand, within RR42_RS02300at 515.607 kb on - strand, within RR42_RS02300at 515.609 kb on + strand, within RR42_RS02300at 515.609 kb on + strand, within RR42_RS02300at 515.609 kb on + strand, within RR42_RS02300at 515.610 kb on - strand, within RR42_RS02300at 515.610 kb on - strand, within RR42_RS02300at 515.824 kb on + strandat 515.843 kb on - strandat 515.897 kb on - strandat 515.922 kb on + strandat 515.923 kb on - strandat 515.923 kb on - strandat 515.933 kb on + strandat 515.933 kb on + strandat 515.933 kb on + strandat 515.934 kb on - strandat 515.934 kb on - strandat 515.945 kb on + strandat 515.946 kb on - strandat 515.946 kb on - strandat 516.216 kb on + strandat 516.216 kb on + strandat 516.216 kb on + strandat 516.216 kb on + strandat 516.216 kb on + strandat 516.217 kb on - strandat 516.217 kb on - strandat 516.217 kb on - strandat 516.217 kb on - strandat 516.218 kb on + strandat 516.218 kb on + strandat 516.218 kb on + strandat 516.219 kb on - strandat 516.219 kb on - strandat 516.219 kb on - strandat 516.294 kb on + strand, within RR42_RS02305at 516.295 kb on - strand, within RR42_RS02305at 516.295 kb on - strand, within RR42_RS02305at 516.295 kb on - strand, within RR42_RS02305at 516.436 kb on + strand, within RR42_RS02305at 516.618 kb on + strand, within RR42_RS02305at 516.618 kb on + strand, within RR42_RS02305at 516.702 kb on + strand, within RR42_RS02305at 516.726 kb on + strand, within RR42_RS02305at 516.726 kb on + strand, within RR42_RS02305at 516.726 kb on + strand, within RR42_RS02305at 516.820 kb on - strand, within RR42_RS02305at 516.850 kb on - strand, within RR42_RS02305at 516.850 kb on - strand, within RR42_RS02305at 517.062 kb on - strand, within RR42_RS02305at 517.062 kb on - strand, within RR42_RS02305at 517.086 kb on + strand, within RR42_RS02305at 517.087 kb on - strand, within RR42_RS02305at 517.190 kb on + strand, within RR42_RS02305at 517.191 kb on - strand, within RR42_RS02305at 517.191 kb on - strand, within RR42_RS02305at 517.215 kb on + strand, within RR42_RS02305at 517.215 kb on + strand, within RR42_RS02305at 517.215 kb on + strand, within RR42_RS02305at 517.216 kb on - strand, within RR42_RS02305at 517.266 kb on + strand, within RR42_RS02305at 517.266 kb on + strand, within RR42_RS02305at 517.267 kb on - strand, within RR42_RS02305at 517.267 kb on - strand, within RR42_RS02305at 517.296 kb on + strandat 517.296 kb on + strandat 517.317 kb on + strandat 517.317 kb on + strandat 517.344 kb on + strandat 517.344 kb on + strandat 517.445 kb on + strandat 517.587 kb on - strand, within RR42_RS02310at 517.700 kb on - strand, within RR42_RS02310at 517.705 kb on - strand, within RR42_RS02310at 517.870 kb on + strand, within RR42_RS02310at 517.870 kb on + strand, within RR42_RS02310at 517.871 kb on - strand, within RR42_RS02310at 518.369 kb on - strandat 518.369 kb on - strandat 518.397 kb on + strandat 518.397 kb on + strandat 518.397 kb on + strandat 518.398 kb on - strandat 518.398 kb on - strandat 518.402 kb on - strandat 518.402 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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515,232 + RR42_RS02300 0.15 -0.1
515,232 + RR42_RS02300 0.15 -0.5
515,232 + RR42_RS02300 0.15 -2.5
515,232 + RR42_RS02300 0.15 -1.3
515,233 - RR42_RS02300 0.15 -0.2
515,233 - RR42_RS02300 0.15 +0.0
515,233 - RR42_RS02300 0.15 -0.3
515,233 - RR42_RS02300 0.15 +0.0
515,241 + RR42_RS02300 0.16 -0.1
515,606 + RR42_RS02300 0.71 +0.0
515,607 - RR42_RS02300 0.71 -3.9
515,607 - RR42_RS02300 0.71 -1.0
515,609 + RR42_RS02300 0.71 -1.9
515,609 + RR42_RS02300 0.71 +1.2
515,609 + RR42_RS02300 0.71 -0.3
515,610 - RR42_RS02300 0.71 +1.4
515,610 - RR42_RS02300 0.71 +0.3
515,824 + -1.3
515,843 - -0.5
515,897 - +0.9
515,922 + +0.3
515,923 - +0.7
515,923 - +0.3
515,933 + +2.0
515,933 + +1.2
515,933 + -0.0
515,934 - +2.1
515,934 - -0.3
515,945 + +0.8
515,946 - +0.6
515,946 - +0.0
516,216 + +1.7
516,216 + +3.1
516,216 + -0.1
516,216 + +0.9
516,216 + -0.7
516,217 - +0.4
516,217 - -0.5
516,217 - +0.1
516,217 - +0.3
516,218 + +1.6
516,218 + +1.9
516,218 + +0.3
516,219 - +0.5
516,219 - -0.3
516,219 - +3.2
516,294 + RR42_RS02305 0.16 -0.0
516,295 - RR42_RS02305 0.16 -3.3
516,295 - RR42_RS02305 0.16 -0.4
516,295 - RR42_RS02305 0.16 -0.2
516,436 + RR42_RS02305 0.26 +1.5
516,618 + RR42_RS02305 0.40 +1.1
516,618 + RR42_RS02305 0.40 +2.6
516,702 + RR42_RS02305 0.46 +0.6
516,726 + RR42_RS02305 0.48 -0.8
516,726 + RR42_RS02305 0.48 -0.4
516,726 + RR42_RS02305 0.48 -1.2
516,820 - RR42_RS02305 0.55 -0.4
516,850 - RR42_RS02305 0.57 -0.3
516,850 - RR42_RS02305 0.57 +0.4
517,062 - RR42_RS02305 0.73 +1.1
517,062 - RR42_RS02305 0.73 +1.4
517,086 + RR42_RS02305 0.75 +1.4
517,087 - RR42_RS02305 0.75 -0.1
517,190 + RR42_RS02305 0.83 -0.9
517,191 - RR42_RS02305 0.83 -0.3
517,191 - RR42_RS02305 0.83 +1.8
517,215 + RR42_RS02305 0.84 -1.0
517,215 + RR42_RS02305 0.84 -3.1
517,215 + RR42_RS02305 0.84 -1.5
517,216 - RR42_RS02305 0.85 +1.2
517,266 + RR42_RS02305 0.88 +1.4
517,266 + RR42_RS02305 0.88 -0.1
517,267 - RR42_RS02305 0.88 -0.0
517,267 - RR42_RS02305 0.88 +0.2
517,296 + +1.1
517,296 + -1.3
517,317 + +1.0
517,317 + -1.7
517,344 + +0.3
517,344 + +1.1
517,445 + -1.1
517,587 - RR42_RS02310 0.10 -0.8
517,700 - RR42_RS02310 0.24 -0.3
517,705 - RR42_RS02310 0.25 +0.9
517,870 + RR42_RS02310 0.44 -0.6
517,870 + RR42_RS02310 0.44 +0.6
517,871 - RR42_RS02310 0.44 +1.3
518,369 - -2.5
518,369 - -1.4
518,397 + +1.2
518,397 + +0.8
518,397 + +0.3
518,398 - -0.4
518,398 - -0.4
518,402 - -1.2
518,402 - -0.0

Or see this region's nucleotide sequence