Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02290 and RR42_RS02295 overlap by 4 nucleotides RR42_RS02295 and RR42_RS02300 are separated by 123 nucleotides
RR42_RS02290: RR42_RS02290 - multidrug ABC transporter ATP-binding protein, at 513,044 to 514,057
_RS02290
RR42_RS02295: RR42_RS02295 - secretion protein HlyD, at 514,054 to 515,007
_RS02295
RR42_RS02300: RR42_RS02300 - hypothetical protein, at 515,131 to 515,802
_RS02300
Position (kb)
514
515
516 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 513.232 kb on + strand, within RR42_RS02290 at 513.394 kb on - strand, within RR42_RS02290 at 513.401 kb on - strand, within RR42_RS02290 at 513.402 kb on + strand, within RR42_RS02290 at 513.403 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.699 kb on + strand, within RR42_RS02290 at 513.700 kb on - strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.772 kb on - strand, within RR42_RS02290 at 513.973 kb on - strand at 514.163 kb on + strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.388 kb on + strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.429 kb on + strand, within RR42_RS02295 at 514.622 kb on + strand, within RR42_RS02295 at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.920 kb on - strand at 514.960 kb on + strand at 514.960 kb on + strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand at 515.232 kb on + strand, within RR42_RS02300 at 515.232 kb on + strand, within RR42_RS02300 at 515.232 kb on + strand, within RR42_RS02300 at 515.232 kb on + strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.233 kb on - strand, within RR42_RS02300 at 515.241 kb on + strand, within RR42_RS02300 at 515.606 kb on + strand, within RR42_RS02300 at 515.607 kb on - strand, within RR42_RS02300 at 515.607 kb on - strand, within RR42_RS02300 at 515.609 kb on + strand, within RR42_RS02300 at 515.609 kb on + strand, within RR42_RS02300 at 515.609 kb on + strand, within RR42_RS02300 at 515.610 kb on - strand, within RR42_RS02300 at 515.610 kb on - strand, within RR42_RS02300 at 515.824 kb on + strand at 515.843 kb on - strand at 515.897 kb on - strand at 515.922 kb on + strand at 515.923 kb on - strand at 515.923 kb on - strand at 515.933 kb on + strand at 515.933 kb on + strand at 515.933 kb on + strand at 515.934 kb on - strand at 515.934 kb on - strand at 515.945 kb on + strand at 515.946 kb on - strand at 515.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 513,232 + RR42_RS02290 0.19 -0.8 513,394 - RR42_RS02290 0.35 -2.1 513,401 - RR42_RS02290 0.35 +1.0 513,402 + RR42_RS02290 0.35 +1.0 513,403 - RR42_RS02290 0.35 -1.3 513,439 - RR42_RS02290 0.39 +0.7 513,439 - RR42_RS02290 0.39 -0.0 513,699 + RR42_RS02290 0.65 -1.8 513,700 - RR42_RS02290 0.65 +0.3 513,771 + RR42_RS02290 0.72 -1.9 513,771 + RR42_RS02290 0.72 -0.8 513,772 - RR42_RS02290 0.72 -1.1 513,973 - -1.6 514,163 + RR42_RS02295 0.11 +1.1 514,164 - RR42_RS02295 0.12 +2.6 514,164 - RR42_RS02295 0.12 -2.6 514,202 + RR42_RS02295 0.16 -0.1 514,202 + RR42_RS02295 0.16 -1.8 514,202 + RR42_RS02295 0.16 +0.0 514,202 + RR42_RS02295 0.16 +0.6 514,202 + RR42_RS02295 0.16 -0.9 514,203 - RR42_RS02295 0.16 +1.6 514,203 - RR42_RS02295 0.16 +0.2 514,203 - RR42_RS02295 0.16 +1.7 514,203 - RR42_RS02295 0.16 -0.8 514,203 - RR42_RS02295 0.16 -0.2 514,298 + RR42_RS02295 0.26 -0.0 514,298 + RR42_RS02295 0.26 +2.2 514,298 + RR42_RS02295 0.26 -1.8 514,299 - RR42_RS02295 0.26 +0.4 514,299 - RR42_RS02295 0.26 -1.8 514,299 - RR42_RS02295 0.26 -2.3 514,388 + RR42_RS02295 0.35 +0.5 514,389 - RR42_RS02295 0.35 -2.2 514,389 - RR42_RS02295 0.35 +0.3 514,429 + RR42_RS02295 0.39 +2.1 514,622 + RR42_RS02295 0.60 +1.5 514,919 + -0.7 514,919 + -2.7 514,919 + +0.8 514,919 + -1.8 514,920 - -3.5 514,960 + +0.0 514,960 + +0.9 514,961 - -3.1 514,961 - +0.2 514,961 - +0.5 514,961 - -0.4 515,232 + RR42_RS02300 0.15 -0.1 515,232 + RR42_RS02300 0.15 -0.5 515,232 + RR42_RS02300 0.15 -2.5 515,232 + RR42_RS02300 0.15 -1.3 515,233 - RR42_RS02300 0.15 -0.2 515,233 - RR42_RS02300 0.15 +0.0 515,233 - RR42_RS02300 0.15 -0.3 515,233 - RR42_RS02300 0.15 +0.0 515,241 + RR42_RS02300 0.16 -0.1 515,606 + RR42_RS02300 0.71 +0.0 515,607 - RR42_RS02300 0.71 -3.9 515,607 - RR42_RS02300 0.71 -1.0 515,609 + RR42_RS02300 0.71 -1.9 515,609 + RR42_RS02300 0.71 +1.2 515,609 + RR42_RS02300 0.71 -0.3 515,610 - RR42_RS02300 0.71 +1.4 515,610 - RR42_RS02300 0.71 +0.3 515,824 + -1.3 515,843 - -0.5 515,897 - +0.9 515,922 + +0.3 515,923 - +0.7 515,923 - +0.3 515,933 + +2.0 515,933 + +1.2 515,933 + -0.0 515,934 - +2.1 515,934 - -0.3 515,945 + +0.8 515,946 - +0.6 515,946 - +0.0
Or see this region's nucleotide sequence