Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02285 and RR42_RS02290 overlap by 4 nucleotides RR42_RS02290 and RR42_RS02295 overlap by 4 nucleotides
RR42_RS02285: RR42_RS02285 - mannose-1-phosphate guanyltransferase, at 511,869 to 513,047
_RS02285
RR42_RS02290: RR42_RS02290 - multidrug ABC transporter ATP-binding protein, at 513,044 to 514,057
_RS02290
RR42_RS02295: RR42_RS02295 - secretion protein HlyD, at 514,054 to 515,007
_RS02295
Position (kb) 513
514
515 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 512.082 kb on + strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.300 kb on - strand, within RR42_RS02285 at 512.458 kb on + strand, within RR42_RS02285 at 512.459 kb on - strand, within RR42_RS02285 at 512.459 kb on - strand, within RR42_RS02285 at 512.773 kb on + strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 513.232 kb on + strand, within RR42_RS02290 at 513.394 kb on - strand, within RR42_RS02290 at 513.401 kb on - strand, within RR42_RS02290 at 513.402 kb on + strand, within RR42_RS02290 at 513.403 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.699 kb on + strand, within RR42_RS02290 at 513.700 kb on - strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.772 kb on - strand, within RR42_RS02290 at 513.973 kb on - strand at 514.163 kb on + strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.388 kb on + strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.429 kb on + strand, within RR42_RS02295 at 514.622 kb on + strand, within RR42_RS02295 at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.920 kb on - strand at 514.960 kb on + strand at 514.960 kb on + strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 512,082 + RR42_RS02285 0.18 +0.3 512,105 - RR42_RS02285 0.20 -2.3 512,105 - RR42_RS02285 0.20 +0.3 512,105 - RR42_RS02285 0.20 -2.7 512,206 + RR42_RS02285 0.29 -0.7 512,206 + RR42_RS02285 0.29 +1.7 512,206 + RR42_RS02285 0.29 -1.9 512,299 + RR42_RS02285 0.36 +1.7 512,299 + RR42_RS02285 0.36 +1.2 512,299 + RR42_RS02285 0.36 +0.7 512,299 + RR42_RS02285 0.36 +0.2 512,299 + RR42_RS02285 0.36 -0.8 512,300 - RR42_RS02285 0.37 +1.2 512,458 + RR42_RS02285 0.50 -1.5 512,459 - RR42_RS02285 0.50 -1.3 512,459 - RR42_RS02285 0.50 -1.1 512,773 + RR42_RS02285 0.77 +0.5 512,774 - RR42_RS02285 0.77 -0.3 512,774 - RR42_RS02285 0.77 -0.0 512,774 - RR42_RS02285 0.77 +0.4 513,232 + RR42_RS02290 0.19 -0.8 513,394 - RR42_RS02290 0.35 -2.1 513,401 - RR42_RS02290 0.35 +1.0 513,402 + RR42_RS02290 0.35 +1.0 513,403 - RR42_RS02290 0.35 -1.3 513,439 - RR42_RS02290 0.39 +0.7 513,439 - RR42_RS02290 0.39 -0.0 513,699 + RR42_RS02290 0.65 -1.8 513,700 - RR42_RS02290 0.65 +0.3 513,771 + RR42_RS02290 0.72 -1.9 513,771 + RR42_RS02290 0.72 -0.8 513,772 - RR42_RS02290 0.72 -1.1 513,973 - -1.6 514,163 + RR42_RS02295 0.11 +1.1 514,164 - RR42_RS02295 0.12 +2.6 514,164 - RR42_RS02295 0.12 -2.6 514,202 + RR42_RS02295 0.16 -0.1 514,202 + RR42_RS02295 0.16 -1.8 514,202 + RR42_RS02295 0.16 +0.0 514,202 + RR42_RS02295 0.16 +0.6 514,202 + RR42_RS02295 0.16 -0.9 514,203 - RR42_RS02295 0.16 +1.6 514,203 - RR42_RS02295 0.16 +0.2 514,203 - RR42_RS02295 0.16 +1.7 514,203 - RR42_RS02295 0.16 -0.8 514,203 - RR42_RS02295 0.16 -0.2 514,298 + RR42_RS02295 0.26 -0.0 514,298 + RR42_RS02295 0.26 +2.2 514,298 + RR42_RS02295 0.26 -1.8 514,299 - RR42_RS02295 0.26 +0.4 514,299 - RR42_RS02295 0.26 -1.8 514,299 - RR42_RS02295 0.26 -2.3 514,388 + RR42_RS02295 0.35 +0.5 514,389 - RR42_RS02295 0.35 -2.2 514,389 - RR42_RS02295 0.35 +0.3 514,429 + RR42_RS02295 0.39 +2.1 514,622 + RR42_RS02295 0.60 +1.5 514,919 + -0.7 514,919 + -2.7 514,919 + +0.8 514,919 + -1.8 514,920 - -3.5 514,960 + +0.0 514,960 + +0.9 514,961 - -3.1 514,961 - +0.2 514,961 - +0.5 514,961 - -0.4
Or see this region's nucleotide sequence