Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02175 and RR42_RS02180 are separated by 8 nucleotides RR42_RS02180 and RR42_RS02185 are separated by 26 nucleotides
RR42_RS02175: RR42_RS02175 - acetolactate synthase, at 483,230 to 484,855
_RS02175
RR42_RS02180: RR42_RS02180 - ABC transporter substrate-binding protein, at 484,864 to 485,850
_RS02180
RR42_RS02185: RR42_RS02185 - fumarate reductase, at 485,877 to 487,604
_RS02185
Position (kb)
484
485
486 Strain fitness (log2 ratio)
-1
0
1
2 at 484.073 kb on + strand, within RR42_RS02175 at 484.073 kb on + strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.597 kb on - strand, within RR42_RS02175 at 484.788 kb on - strand at 484.788 kb on - strand at 484.865 kb on + strand at 484.866 kb on - strand at 484.904 kb on + strand at 484.904 kb on + strand at 484.905 kb on - strand at 485.072 kb on + strand, within RR42_RS02180 at 485.101 kb on + strand, within RR42_RS02180 at 485.202 kb on + strand, within RR42_RS02180 at 485.257 kb on + strand, within RR42_RS02180 at 485.268 kb on + strand, within RR42_RS02180 at 485.269 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.519 kb on + strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.751 kb on + strand, within RR42_RS02180 at 485.866 kb on + strand at 485.867 kb on - strand at 485.874 kb on - strand at 486.547 kb on + strand, within RR42_RS02185 at 486.548 kb on - strand, within RR42_RS02185 at 486.548 kb on - strand, within RR42_RS02185 at 486.673 kb on + strand, within RR42_RS02185 at 486.674 kb on - strand, within RR42_RS02185 at 486.674 kb on - strand, within RR42_RS02185 at 486.718 kb on + strand, within RR42_RS02185 at 486.719 kb on - strand, within RR42_RS02185 at 486.719 kb on - strand, within RR42_RS02185 at 486.770 kb on - strand, within RR42_RS02185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 484,073 + RR42_RS02175 0.52 +0.7 484,073 + RR42_RS02175 0.52 -1.0 484,074 - RR42_RS02175 0.52 +1.7 484,074 - RR42_RS02175 0.52 +0.6 484,597 - RR42_RS02175 0.84 +0.0 484,788 - +0.2 484,788 - -0.6 484,865 + -0.3 484,866 - +0.4 484,904 + +0.4 484,904 + -0.2 484,905 - +1.8 485,072 + RR42_RS02180 0.21 +1.8 485,101 + RR42_RS02180 0.24 -0.3 485,202 + RR42_RS02180 0.34 -1.7 485,257 + RR42_RS02180 0.40 +0.9 485,268 + RR42_RS02180 0.41 -0.8 485,269 - RR42_RS02180 0.41 -0.2 485,300 - RR42_RS02180 0.44 -0.6 485,300 - RR42_RS02180 0.44 +0.3 485,384 + RR42_RS02180 0.53 -0.6 485,384 + RR42_RS02180 0.53 -0.1 485,384 + RR42_RS02180 0.53 +0.2 485,384 + RR42_RS02180 0.53 -1.1 485,385 - RR42_RS02180 0.53 -1.4 485,385 - RR42_RS02180 0.53 +1.0 485,519 + RR42_RS02180 0.66 +1.0 485,520 - RR42_RS02180 0.66 +0.8 485,520 - RR42_RS02180 0.66 -0.1 485,520 - RR42_RS02180 0.66 -0.8 485,736 - RR42_RS02180 0.88 +1.1 485,736 - RR42_RS02180 0.88 -0.4 485,736 - RR42_RS02180 0.88 +0.6 485,751 + RR42_RS02180 0.90 +0.1 485,866 + +1.6 485,867 - +0.5 485,874 - +1.1 486,547 + RR42_RS02185 0.39 +0.8 486,548 - RR42_RS02185 0.39 -0.0 486,548 - RR42_RS02185 0.39 +0.5 486,673 + RR42_RS02185 0.46 +1.4 486,674 - RR42_RS02185 0.46 -0.4 486,674 - RR42_RS02185 0.46 +0.7 486,718 + RR42_RS02185 0.49 +0.4 486,719 - RR42_RS02185 0.49 +0.4 486,719 - RR42_RS02185 0.49 +1.1 486,770 - RR42_RS02185 0.52 -0.5
Or see this region's nucleotide sequence