Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02165 and RR42_RS02170 are separated by 215 nucleotides RR42_RS02170 and RR42_RS02175 are separated by 20 nucleotides RR42_RS02175 and RR42_RS02180 are separated by 8 nucleotides
RR42_RS02165: RR42_RS02165 - LysR family transcriptional regulator, at 481,318 to 482,256
_RS02165
RR42_RS02170: RR42_RS02170 - hypothetical protein, at 482,472 to 483,209
_RS02170
RR42_RS02175: RR42_RS02175 - acetolactate synthase, at 483,230 to 484,855
_RS02175
RR42_RS02180: RR42_RS02180 - ABC transporter substrate-binding protein, at 484,864 to 485,850
_RS02180
Position (kb) 483
484
485 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 482.261 kb on - strand at 482.293 kb on + strand at 482.360 kb on - strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.399 kb on - strand at 482.435 kb on + strand at 482.521 kb on - strand at 482.521 kb on - strand at 482.522 kb on + strand at 482.869 kb on + strand, within RR42_RS02170 at 482.869 kb on + strand, within RR42_RS02170 at 482.872 kb on + strand, within RR42_RS02170 at 482.916 kb on + strand, within RR42_RS02170 at 482.917 kb on - strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 483.033 kb on + strand, within RR42_RS02170 at 483.034 kb on - strand, within RR42_RS02170 at 483.034 kb on - strand, within RR42_RS02170 at 483.041 kb on + strand, within RR42_RS02170 at 483.093 kb on + strand, within RR42_RS02170 at 483.358 kb on - strand at 483.387 kb on + strand at 483.433 kb on - strand, within RR42_RS02175 at 483.454 kb on - strand, within RR42_RS02175 at 484.073 kb on + strand, within RR42_RS02175 at 484.073 kb on + strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.597 kb on - strand, within RR42_RS02175 at 484.788 kb on - strand at 484.788 kb on - strand at 484.865 kb on + strand at 484.866 kb on - strand at 484.904 kb on + strand at 484.904 kb on + strand at 484.905 kb on - strand at 485.072 kb on + strand, within RR42_RS02180 at 485.101 kb on + strand, within RR42_RS02180 at 485.202 kb on + strand, within RR42_RS02180 at 485.257 kb on + strand, within RR42_RS02180 at 485.268 kb on + strand, within RR42_RS02180 at 485.269 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.519 kb on + strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.751 kb on + strand, within RR42_RS02180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 482,261 - +0.5 482,293 + -0.6 482,360 - -0.6 482,397 + -0.3 482,397 + +0.2 482,397 + -1.1 482,397 + -1.6 482,398 - -0.0 482,398 - +1.2 482,398 - -0.1 482,398 - -0.1 482,398 - -2.3 482,399 - +1.6 482,435 + -1.4 482,521 - +2.5 482,521 - -0.3 482,522 + -0.3 482,869 + RR42_RS02170 0.54 -1.0 482,869 + RR42_RS02170 0.54 -0.7 482,872 + RR42_RS02170 0.54 -0.6 482,916 + RR42_RS02170 0.60 -2.8 482,917 - RR42_RS02170 0.60 -2.1 482,925 + RR42_RS02170 0.61 +1.0 482,925 + RR42_RS02170 0.61 -1.3 482,925 + RR42_RS02170 0.61 -1.9 483,033 + RR42_RS02170 0.76 +0.4 483,034 - RR42_RS02170 0.76 +0.7 483,034 - RR42_RS02170 0.76 -0.4 483,041 + RR42_RS02170 0.77 -0.4 483,093 + RR42_RS02170 0.84 +1.4 483,358 - -0.2 483,387 + +0.2 483,433 - RR42_RS02175 0.12 -0.6 483,454 - RR42_RS02175 0.14 -0.6 484,073 + RR42_RS02175 0.52 +0.7 484,073 + RR42_RS02175 0.52 -1.0 484,074 - RR42_RS02175 0.52 +1.7 484,074 - RR42_RS02175 0.52 +0.6 484,597 - RR42_RS02175 0.84 +0.0 484,788 - +0.2 484,788 - -0.6 484,865 + -0.3 484,866 - +0.4 484,904 + +0.4 484,904 + -0.2 484,905 - +1.8 485,072 + RR42_RS02180 0.21 +1.8 485,101 + RR42_RS02180 0.24 -0.3 485,202 + RR42_RS02180 0.34 -1.7 485,257 + RR42_RS02180 0.40 +0.9 485,268 + RR42_RS02180 0.41 -0.8 485,269 - RR42_RS02180 0.41 -0.2 485,300 - RR42_RS02180 0.44 -0.6 485,300 - RR42_RS02180 0.44 +0.3 485,384 + RR42_RS02180 0.53 -0.6 485,384 + RR42_RS02180 0.53 -0.1 485,384 + RR42_RS02180 0.53 +0.2 485,384 + RR42_RS02180 0.53 -1.1 485,385 - RR42_RS02180 0.53 -1.4 485,385 - RR42_RS02180 0.53 +1.0 485,519 + RR42_RS02180 0.66 +1.0 485,520 - RR42_RS02180 0.66 +0.8 485,520 - RR42_RS02180 0.66 -0.1 485,520 - RR42_RS02180 0.66 -0.8 485,736 - RR42_RS02180 0.88 +1.1 485,736 - RR42_RS02180 0.88 -0.4 485,736 - RR42_RS02180 0.88 +0.6 485,751 + RR42_RS02180 0.90 +0.1
Or see this region's nucleotide sequence