Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02060 and RR42_RS02065 are separated by 119 nucleotides RR42_RS02065 and RR42_RS02070 are separated by 191 nucleotides RR42_RS02070 and RR42_RS02075 are separated by 35 nucleotides RR42_RS02075 and RR42_RS02080 are separated by 384 nucleotides
RR42_RS02060: RR42_RS02060 - nucleotide-binding protein, at 459,418 to 460,335
_RS02060
RR42_RS02065: RR42_RS02065 - phosphate starvation-inducible protein PsiF, at 460,455 to 460,778
_RS02065
RR42_RS02070: RR42_RS02070 - HPr kinase, at 460,970 to 461,941
_RS02070
RR42_RS02075: RR42_RS02075 - PTS sugar transporter subunit IIA, at 461,977 to 462,432
_RS02075
RR42_RS02080: RR42_RS02080 - ribosome hibernation promoting factor, at 462,817 to 463,167
_RS02080
Position (kb)
460
461
462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 460.050 kb on - strand, within RR42_RS02060 at 460.050 kb on - strand, within RR42_RS02060 at 460.071 kb on + strand, within RR42_RS02060 at 460.072 kb on - strand, within RR42_RS02060 at 460.209 kb on - strand, within RR42_RS02060 at 460.209 kb on - strand, within RR42_RS02060 at 460.209 kb on - strand, within RR42_RS02060 at 460.260 kb on - strand at 460.276 kb on + strand at 460.276 kb on + strand at 460.277 kb on - strand at 460.277 kb on - strand at 460.277 kb on - strand at 460.277 kb on - strand at 460.320 kb on + strand at 460.320 kb on + strand at 460.320 kb on + strand at 460.321 kb on - strand at 460.321 kb on - strand at 460.321 kb on - strand at 460.321 kb on - strand at 460.324 kb on + strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.335 kb on + strand at 460.335 kb on + strand at 460.349 kb on + strand at 460.349 kb on + strand at 460.350 kb on - strand at 460.391 kb on - strand at 460.423 kb on + strand at 460.451 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.457 kb on - strand at 460.457 kb on - strand at 460.577 kb on + strand, within RR42_RS02065 at 460.578 kb on - strand, within RR42_RS02065 at 460.858 kb on + strand at 460.859 kb on - strand at 460.915 kb on + strand at 460.915 kb on + strand at 460.915 kb on + strand at 460.916 kb on - strand at 460.916 kb on - strand at 460.916 kb on - strand at 460.916 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 462.454 kb on - strand at 462.454 kb on - strand at 462.568 kb on - strand at 462.568 kb on - strand at 462.569 kb on + strand at 462.569 kb on + strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.603 kb on + strand at 462.604 kb on - strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.661 kb on + strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.673 kb on + strand at 462.673 kb on + strand at 462.674 kb on - strand at 462.674 kb on - strand at 462.674 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.685 kb on + strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.722 kb on - strand at 462.722 kb on - strand at 462.722 kb on - strand at 462.797 kb on - strand at 462.849 kb on - strand at 462.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 460,050 - RR42_RS02060 0.69 -1.1 460,050 - RR42_RS02060 0.69 +0.7 460,071 + RR42_RS02060 0.71 -0.6 460,072 - RR42_RS02060 0.71 -0.0 460,209 - RR42_RS02060 0.86 -0.6 460,209 - RR42_RS02060 0.86 -1.1 460,209 - RR42_RS02060 0.86 +1.2 460,260 - -2.3 460,276 + -3.1 460,276 + +0.0 460,277 - -1.5 460,277 - -1.2 460,277 - -2.1 460,277 - -0.8 460,320 + +0.9 460,320 + +0.9 460,320 + -0.1 460,321 - +1.7 460,321 - -0.3 460,321 - -1.1 460,321 - +1.8 460,324 + -1.8 460,325 - +0.8 460,325 - -0.4 460,325 - +1.0 460,325 - -1.5 460,325 - +0.4 460,325 - -0.8 460,325 - -0.6 460,325 - -0.8 460,335 + -0.0 460,335 + -0.3 460,349 + -0.3 460,349 + +1.3 460,350 - -2.8 460,391 - +0.5 460,423 + +0.3 460,451 + -0.8 460,456 + +0.2 460,456 + -2.3 460,456 + +0.4 460,456 + -0.6 460,456 + -2.1 460,457 - -0.1 460,457 - -0.4 460,577 + RR42_RS02065 0.38 -1.9 460,578 - RR42_RS02065 0.38 -1.0 460,858 + -0.1 460,859 - +0.7 460,915 + +0.8 460,915 + +0.7 460,915 + -2.6 460,916 - +0.5 460,916 - +0.4 460,916 - +0.4 460,916 - -0.5 460,972 - -1.1 460,972 - -3.0 460,972 - +0.7 460,972 - -0.6 460,972 - -1.6 462,454 - +2.9 462,454 - +2.4 462,568 - -1.9 462,568 - +0.0 462,569 + +1.0 462,569 + +2.2 462,570 - -0.1 462,570 - -2.1 462,570 - +0.0 462,570 - +1.7 462,570 - -3.3 462,570 - -1.4 462,570 - +2.0 462,570 - -1.9 462,570 - -3.1 462,603 + +1.9 462,604 - -1.2 462,657 + +1.6 462,657 + +2.1 462,657 + -1.6 462,657 + -0.9 462,657 + -0.8 462,658 - +0.2 462,658 - -0.9 462,658 - -1.8 462,658 - +0.6 462,658 - -0.4 462,661 + +0.4 462,662 - +0.4 462,662 - -1.6 462,662 - +0.9 462,662 - -1.1 462,662 - -0.0 462,673 + +0.8 462,673 + +1.1 462,674 - +0.5 462,674 - -0.9 462,674 - -0.8 462,682 - +0.4 462,682 - +0.0 462,682 - +0.2 462,682 - -2.8 462,682 - +0.4 462,685 + -0.8 462,686 - -0.7 462,686 - -1.8 462,686 - +1.2 462,686 - -0.4 462,722 - -0.2 462,722 - -2.3 462,722 - -0.0 462,797 - -0.2 462,849 - +1.7 462,849 - -2.7
Or see this region's nucleotide sequence