Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01955 and RR42_RS01960 are separated by 362 nucleotides RR42_RS01960 and RR42_RS01965 are separated by 208 nucleotides RR42_RS01965 and RR42_RS01970 are separated by 7 nucleotides
RR42_RS01955: RR42_RS01955 - nitrate transporter, at 437,726 to 439,045
_RS01955
RR42_RS01960: RR42_RS01960 - transcriptional regulator, at 439,408 to 440,061
_RS01960
RR42_RS01965: RR42_RS01965 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 440,270 to 440,971
_RS01965
RR42_RS01970: RR42_RS01970 - maleylacetoacetate isomerase, at 440,979 to 441,623
_RS01970
Position (kb) 439
440
441 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 438.444 kb on + strand, within RR42_RS01955 at 438.445 kb on - strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.447 kb on + strand, within RR42_RS01955 at 438.448 kb on - strand, within RR42_RS01955 at 438.495 kb on + strand, within RR42_RS01955 at 438.657 kb on + strand, within RR42_RS01955 at 438.657 kb on + strand, within RR42_RS01955 at 438.657 kb on + strand, within RR42_RS01955 at 438.657 kb on + strand, within RR42_RS01955 at 438.657 kb on + strand, within RR42_RS01955 at 438.658 kb on - strand, within RR42_RS01955 at 438.658 kb on - strand, within RR42_RS01955 at 438.658 kb on - strand, within RR42_RS01955 at 438.658 kb on - strand, within RR42_RS01955 at 438.658 kb on - strand, within RR42_RS01955 at 438.658 kb on - strand, within RR42_RS01955 at 438.670 kb on - strand, within RR42_RS01955 at 438.762 kb on + strand, within RR42_RS01955 at 438.762 kb on + strand, within RR42_RS01955 at 438.763 kb on - strand, within RR42_RS01955 at 438.763 kb on - strand, within RR42_RS01955 at 438.763 kb on - strand, within RR42_RS01955 at 438.763 kb on - strand, within RR42_RS01955 at 438.763 kb on - strand, within RR42_RS01955 at 438.887 kb on + strand, within RR42_RS01955 at 439.167 kb on + strand at 439.168 kb on - strand at 439.168 kb on - strand at 439.409 kb on + strand at 439.409 kb on + strand at 439.410 kb on - strand at 439.490 kb on + strand, within RR42_RS01960 at 439.490 kb on + strand, within RR42_RS01960 at 439.490 kb on + strand, within RR42_RS01960 at 439.490 kb on + strand, within RR42_RS01960 at 439.490 kb on + strand, within RR42_RS01960 at 439.491 kb on - strand, within RR42_RS01960 at 439.491 kb on - strand, within RR42_RS01960 at 439.625 kb on + strand, within RR42_RS01960 at 439.679 kb on + strand, within RR42_RS01960 at 439.679 kb on + strand, within RR42_RS01960 at 439.680 kb on - strand, within RR42_RS01960 at 439.680 kb on - strand, within RR42_RS01960 at 440.119 kb on + strand at 440.120 kb on - strand at 440.120 kb on - strand at 440.120 kb on - strand at 440.177 kb on + strand at 440.177 kb on + strand at 440.177 kb on + strand at 440.177 kb on + strand at 440.178 kb on - strand at 440.178 kb on - strand at 440.178 kb on - strand at 440.178 kb on - strand at 440.178 kb on - strand at 440.178 kb on - strand at 440.182 kb on + strand at 440.183 kb on - strand at 440.183 kb on - strand at 440.183 kb on - strand at 440.183 kb on - strand at 440.183 kb on - strand at 440.236 kb on + strand at 440.236 kb on + strand at 440.237 kb on - strand at 440.237 kb on - strand at 440.242 kb on - strand at 440.242 kb on - strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.253 kb on + strand at 440.254 kb on - strand at 440.254 kb on - strand at 440.254 kb on - strand at 440.283 kb on + strand at 440.283 kb on + strand at 440.283 kb on + strand at 440.284 kb on - strand at 440.284 kb on - strand at 440.354 kb on + strand, within RR42_RS01965 at 440.372 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.456 kb on + strand, within RR42_RS01965 at 440.516 kb on + strand, within RR42_RS01965 at 440.517 kb on - strand, within RR42_RS01965 at 440.831 kb on + strand, within RR42_RS01965 at 440.831 kb on + strand, within RR42_RS01965 at 440.831 kb on + strand, within RR42_RS01965 at 440.832 kb on - strand, within RR42_RS01965 at 440.873 kb on + strand, within RR42_RS01965 at 440.873 kb on + strand, within RR42_RS01965 at 440.873 kb on + strand, within RR42_RS01965 at 440.873 kb on + strand, within RR42_RS01965 at 440.873 kb on + strand, within RR42_RS01965 at 440.874 kb on - strand, within RR42_RS01965 at 440.874 kb on - strand, within RR42_RS01965 at 440.874 kb on - strand, within RR42_RS01965 at 440.964 kb on + strand at 440.964 kb on + strand at 440.964 kb on + strand at 440.965 kb on - strand at 440.965 kb on - strand at 440.988 kb on + strand at 440.989 kb on - strand at 440.995 kb on - strand at 440.995 kb on - strand at 441.054 kb on + strand, within RR42_RS01970 at 441.055 kb on - strand, within RR42_RS01970 at 441.055 kb on - strand, within RR42_RS01970 at 441.055 kb on - strand, within RR42_RS01970 at 441.060 kb on + strand, within RR42_RS01970 at 441.060 kb on + strand, within RR42_RS01970 at 441.061 kb on - strand, within RR42_RS01970 at 441.061 kb on - strand, within RR42_RS01970 at 441.061 kb on - strand, within RR42_RS01970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 438,444 + RR42_RS01955 0.54 +0.6 438,445 - RR42_RS01955 0.54 -1.6 438,447 + RR42_RS01955 0.55 -1.1 438,447 + RR42_RS01955 0.55 -2.7 438,447 + RR42_RS01955 0.55 -0.7 438,447 + RR42_RS01955 0.55 -2.7 438,447 + RR42_RS01955 0.55 -0.2 438,447 + RR42_RS01955 0.55 -2.2 438,447 + RR42_RS01955 0.55 +0.0 438,448 - RR42_RS01955 0.55 -1.6 438,495 + RR42_RS01955 0.58 -1.3 438,657 + RR42_RS01955 0.71 -0.1 438,657 + RR42_RS01955 0.71 +2.0 438,657 + RR42_RS01955 0.71 +0.8 438,657 + RR42_RS01955 0.71 -1.1 438,657 + RR42_RS01955 0.71 +0.3 438,658 - RR42_RS01955 0.71 -0.2 438,658 - RR42_RS01955 0.71 +0.6 438,658 - RR42_RS01955 0.71 +0.5 438,658 - RR42_RS01955 0.71 -0.8 438,658 - RR42_RS01955 0.71 +1.2 438,658 - RR42_RS01955 0.71 +0.0 438,670 - RR42_RS01955 0.72 -3.9 438,762 + RR42_RS01955 0.78 -2.9 438,762 + RR42_RS01955 0.78 -1.1 438,763 - RR42_RS01955 0.79 +0.5 438,763 - RR42_RS01955 0.79 +0.8 438,763 - RR42_RS01955 0.79 +0.7 438,763 - RR42_RS01955 0.79 -1.1 438,763 - RR42_RS01955 0.79 +0.6 438,887 + RR42_RS01955 0.88 +0.3 439,167 + -0.4 439,168 - -1.4 439,168 - +1.2 439,409 + +1.8 439,409 + +0.1 439,410 - -0.7 439,490 + RR42_RS01960 0.13 -2.2 439,490 + RR42_RS01960 0.13 -0.6 439,490 + RR42_RS01960 0.13 -0.3 439,490 + RR42_RS01960 0.13 -0.2 439,490 + RR42_RS01960 0.13 -1.1 439,491 - RR42_RS01960 0.13 -0.6 439,491 - RR42_RS01960 0.13 +0.2 439,625 + RR42_RS01960 0.33 +0.2 439,679 + RR42_RS01960 0.41 +1.2 439,679 + RR42_RS01960 0.41 -0.3 439,680 - RR42_RS01960 0.42 -1.3 439,680 - RR42_RS01960 0.42 +1.8 440,119 + -0.0 440,120 - +1.0 440,120 - -0.1 440,120 - -1.2 440,177 + -2.3 440,177 + -1.8 440,177 + +1.3 440,177 + -2.7 440,178 - +0.4 440,178 - -0.8 440,178 - +1.0 440,178 - -1.0 440,178 - -1.1 440,178 - +1.4 440,182 + -0.5 440,183 - +2.0 440,183 - -3.6 440,183 - +0.7 440,183 - -0.7 440,183 - +0.3 440,236 + -1.2 440,236 + +1.9 440,237 - +1.8 440,237 - +0.1 440,242 - +0.2 440,242 - +0.5 440,253 + +3.2 440,253 + +0.5 440,253 + -0.3 440,253 + -0.3 440,253 + -1.1 440,253 + +0.2 440,253 + -0.1 440,253 + -1.1 440,253 + -2.0 440,254 - +0.7 440,254 - +0.4 440,254 - +0.5 440,283 + +0.1 440,283 + -0.8 440,283 + +3.6 440,284 - +0.8 440,284 - +0.1 440,354 + RR42_RS01965 0.12 +0.6 440,372 + RR42_RS01965 0.15 +0.1 440,456 + RR42_RS01965 0.26 +0.2 440,456 + RR42_RS01965 0.26 +1.0 440,456 + RR42_RS01965 0.26 -2.5 440,456 + RR42_RS01965 0.26 +0.8 440,456 + RR42_RS01965 0.26 -3.3 440,456 + RR42_RS01965 0.26 +0.7 440,456 + RR42_RS01965 0.26 +0.7 440,456 + RR42_RS01965 0.26 +1.0 440,516 + RR42_RS01965 0.35 -1.2 440,517 - RR42_RS01965 0.35 -2.1 440,831 + RR42_RS01965 0.80 -0.2 440,831 + RR42_RS01965 0.80 -3.7 440,831 + RR42_RS01965 0.80 -0.5 440,832 - RR42_RS01965 0.80 +0.1 440,873 + RR42_RS01965 0.86 -0.2 440,873 + RR42_RS01965 0.86 +0.5 440,873 + RR42_RS01965 0.86 -2.9 440,873 + RR42_RS01965 0.86 +3.2 440,873 + RR42_RS01965 0.86 -0.3 440,874 - RR42_RS01965 0.86 -1.1 440,874 - RR42_RS01965 0.86 +0.8 440,874 - RR42_RS01965 0.86 -1.6 440,964 + -1.6 440,964 + +0.8 440,964 + -1.3 440,965 - +0.9 440,965 - +0.5 440,988 + -0.9 440,989 - +2.5 440,995 - +1.7 440,995 - +1.1 441,054 + RR42_RS01970 0.12 +1.3 441,055 - RR42_RS01970 0.12 +1.3 441,055 - RR42_RS01970 0.12 +1.9 441,055 - RR42_RS01970 0.12 -0.2 441,060 + RR42_RS01970 0.13 +1.1 441,060 + RR42_RS01970 0.13 +0.2 441,061 - RR42_RS01970 0.13 +0.3 441,061 - RR42_RS01970 0.13 +1.0 441,061 - RR42_RS01970 0.13 +0.8
Or see this region's nucleotide sequence