Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01795 and RR42_RS01800 are separated by 643 nucleotides RR42_RS01800 and RR42_RS01805 are separated by 272 nucleotides
RR42_RS01795: RR42_RS01795 - phosphoenolpyruvate-protein phosphotransferase, at 406,915 to 408,690
_RS01795
RR42_RS01800: RR42_RS01800 - bacterioferritin, at 409,334 to 409,813
_RS01800
RR42_RS01805: RR42_RS01805 - selenophosphate synthase, at 410,086 to 411,153
_RS01805
Position (kb)
409
410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 408.358 kb on + strand, within RR42_RS01795 at 408.358 kb on + strand, within RR42_RS01795 at 408.359 kb on - strand, within RR42_RS01795 at 408.359 kb on - strand, within RR42_RS01795 at 408.359 kb on - strand, within RR42_RS01795 at 408.403 kb on + strand, within RR42_RS01795 at 408.403 kb on + strand, within RR42_RS01795 at 408.404 kb on - strand, within RR42_RS01795 at 408.404 kb on - strand, within RR42_RS01795 at 408.649 kb on + strand at 408.649 kb on + strand at 408.649 kb on + strand at 408.649 kb on + strand at 408.649 kb on + strand at 408.650 kb on - strand at 408.650 kb on - strand at 408.650 kb on - strand at 408.716 kb on - strand at 408.735 kb on + strand at 408.735 kb on + strand at 408.735 kb on + strand at 408.736 kb on - strand at 408.739 kb on + strand at 408.799 kb on - strand at 408.820 kb on + strand at 408.820 kb on + strand at 408.821 kb on - strand at 408.821 kb on - strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.877 kb on - strand at 408.928 kb on + strand at 408.929 kb on - strand at 408.983 kb on + strand at 408.983 kb on + strand at 408.984 kb on - strand at 408.984 kb on - strand at 409.019 kb on + strand at 409.019 kb on + strand at 409.406 kb on + strand, within RR42_RS01800 at 409.407 kb on - strand, within RR42_RS01800 at 409.407 kb on - strand, within RR42_RS01800 at 409.407 kb on - strand, within RR42_RS01800 at 409.427 kb on + strand, within RR42_RS01800 at 409.428 kb on - strand, within RR42_RS01800 at 409.428 kb on - strand, within RR42_RS01800 at 409.466 kb on + strand, within RR42_RS01800 at 409.466 kb on + strand, within RR42_RS01800 at 409.466 kb on + strand, within RR42_RS01800 at 409.467 kb on - strand, within RR42_RS01800 at 409.467 kb on - strand, within RR42_RS01800 at 409.467 kb on - strand, within RR42_RS01800 at 409.648 kb on + strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.652 kb on + strand, within RR42_RS01800 at 409.653 kb on - strand, within RR42_RS01800 at 409.653 kb on - strand, within RR42_RS01800 at 409.653 kb on - strand, within RR42_RS01800 at 409.674 kb on - strand, within RR42_RS01800 at 409.778 kb on + strand at 409.778 kb on + strand at 409.779 kb on - strand at 409.779 kb on - strand at 409.779 kb on - strand at 409.811 kb on + strand at 409.811 kb on + strand at 409.811 kb on + strand at 410.002 kb on + strand at 410.003 kb on - strand at 410.003 kb on - strand at 410.113 kb on + strand at 410.114 kb on - strand at 410.114 kb on - strand at 410.245 kb on + strand, within RR42_RS01805 at 410.368 kb on + strand, within RR42_RS01805 at 410.368 kb on + strand, within RR42_RS01805 at 410.368 kb on + strand, within RR42_RS01805 at 410.368 kb on + strand, within RR42_RS01805 at 410.368 kb on + strand, within RR42_RS01805 at 410.368 kb on + strand, within RR42_RS01805 at 410.369 kb on - strand, within RR42_RS01805 at 410.369 kb on - strand, within RR42_RS01805 at 410.369 kb on - strand, within RR42_RS01805 at 410.369 kb on - strand, within RR42_RS01805 at 410.369 kb on - strand, within RR42_RS01805 at 410.614 kb on + strand, within RR42_RS01805 at 410.614 kb on + strand, within RR42_RS01805 at 410.614 kb on + strand, within RR42_RS01805 at 410.615 kb on - strand, within RR42_RS01805 at 410.615 kb on - strand, within RR42_RS01805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 408,358 + RR42_RS01795 0.81 +0.3 408,358 + RR42_RS01795 0.81 -1.4 408,359 - RR42_RS01795 0.81 -3.6 408,359 - RR42_RS01795 0.81 -3.3 408,359 - RR42_RS01795 0.81 -1.2 408,403 + RR42_RS01795 0.84 -0.8 408,403 + RR42_RS01795 0.84 -3.2 408,404 - RR42_RS01795 0.84 -2.7 408,404 - RR42_RS01795 0.84 +2.7 408,649 + +0.5 408,649 + -1.0 408,649 + -0.8 408,649 + -2.5 408,649 + +0.2 408,650 - -3.2 408,650 - -1.3 408,650 - -1.6 408,716 - +3.2 408,735 + +0.3 408,735 + -1.1 408,735 + -2.3 408,736 - +0.8 408,739 + -0.8 408,799 - +2.2 408,820 + +0.7 408,820 + -0.1 408,821 - +2.0 408,821 - +1.1 408,876 + -2.8 408,876 + +1.5 408,876 + -0.6 408,876 + +0.0 408,876 + -0.3 408,877 - -1.6 408,928 + -1.1 408,929 - -1.5 408,983 + +0.6 408,983 + +0.2 408,984 - -3.1 408,984 - +1.3 409,019 + -1.3 409,019 + -0.0 409,406 + RR42_RS01800 0.15 -0.2 409,407 - RR42_RS01800 0.15 -1.5 409,407 - RR42_RS01800 0.15 -2.8 409,407 - RR42_RS01800 0.15 -0.4 409,427 + RR42_RS01800 0.19 +0.7 409,428 - RR42_RS01800 0.20 -0.8 409,428 - RR42_RS01800 0.20 -1.9 409,466 + RR42_RS01800 0.28 -3.3 409,466 + RR42_RS01800 0.28 -2.6 409,466 + RR42_RS01800 0.28 +2.2 409,467 - RR42_RS01800 0.28 -3.3 409,467 - RR42_RS01800 0.28 -1.1 409,467 - RR42_RS01800 0.28 -2.3 409,648 + RR42_RS01800 0.65 +1.2 409,649 - RR42_RS01800 0.66 -1.1 409,649 - RR42_RS01800 0.66 +0.9 409,649 - RR42_RS01800 0.66 -1.9 409,649 - RR42_RS01800 0.66 -1.2 409,652 + RR42_RS01800 0.66 +0.2 409,653 - RR42_RS01800 0.66 -2.8 409,653 - RR42_RS01800 0.66 -1.3 409,653 - RR42_RS01800 0.66 -1.6 409,674 - RR42_RS01800 0.71 +1.1 409,778 + +0.2 409,778 + +1.2 409,779 - -1.2 409,779 - +1.0 409,779 - +0.3 409,811 + +0.6 409,811 + +1.2 409,811 + -0.6 410,002 + +0.5 410,003 - -1.1 410,003 - -1.1 410,113 + -0.2 410,114 - -1.4 410,114 - -0.3 410,245 + RR42_RS01805 0.15 +1.7 410,368 + RR42_RS01805 0.26 -1.8 410,368 + RR42_RS01805 0.26 -0.8 410,368 + RR42_RS01805 0.26 +0.9 410,368 + RR42_RS01805 0.26 -0.6 410,368 + RR42_RS01805 0.26 +0.7 410,368 + RR42_RS01805 0.26 -2.3 410,369 - RR42_RS01805 0.26 -0.3 410,369 - RR42_RS01805 0.26 -2.6 410,369 - RR42_RS01805 0.26 -0.5 410,369 - RR42_RS01805 0.26 -0.4 410,369 - RR42_RS01805 0.26 +0.1 410,614 + RR42_RS01805 0.49 -2.3 410,614 + RR42_RS01805 0.49 +1.2 410,614 + RR42_RS01805 0.49 -1.2 410,615 - RR42_RS01805 0.50 +0.9 410,615 - RR42_RS01805 0.50 +1.2
Or see this region's nucleotide sequence