Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01560 and RR42_RS01565 are separated by 362 nucleotides RR42_RS01565 and RR42_RS01570 are separated by 39 nucleotides RR42_RS01570 and RR42_RS01575 are separated by 355 nucleotides
RR42_RS01560: RR42_RS01560 - diguanylate cyclase, at 350,847 to 352,628
_RS01560
RR42_RS01565: RR42_RS01565 - hypothetical protein, at 352,991 to 353,563
_RS01565
RR42_RS01570: RR42_RS01570 - GntR family transcriptional regulator, at 353,603 to 355,120
_RS01570
RR42_RS01575: RR42_RS01575 - omega amino acid--pyruvate aminotransferase, at 355,476 to 356,807
_RS01575
Position (kb)
353
354
355
356 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 352.685 kb on + strand at 352.685 kb on + strand at 352.686 kb on - strand at 352.736 kb on + strand at 352.737 kb on - strand at 352.754 kb on + strand at 352.765 kb on - strand at 352.769 kb on - strand at 352.821 kb on + strand at 352.821 kb on + strand at 352.822 kb on - strand at 352.901 kb on - strand at 352.968 kb on + strand at 352.968 kb on + strand at 352.969 kb on - strand at 352.969 kb on - strand at 352.969 kb on - strand at 352.969 kb on - strand at 352.996 kb on + strand at 352.997 kb on - strand at 353.034 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.144 kb on + strand, within RR42_RS01565 at 353.144 kb on + strand, within RR42_RS01565 at 353.144 kb on + strand, within RR42_RS01565 at 353.145 kb on - strand, within RR42_RS01565 at 353.145 kb on - strand, within RR42_RS01565 at 353.255 kb on - strand, within RR42_RS01565 at 353.371 kb on + strand, within RR42_RS01565 at 353.372 kb on - strand, within RR42_RS01565 at 353.372 kb on - strand, within RR42_RS01565 at 353.480 kb on + strand, within RR42_RS01565 at 353.543 kb on + strand at 353.544 kb on - strand at 353.544 kb on - strand at 353.544 kb on - strand at 353.759 kb on + strand, within RR42_RS01570 at 353.793 kb on + strand, within RR42_RS01570 at 353.794 kb on - strand, within RR42_RS01570 at 354.018 kb on + strand, within RR42_RS01570 at 354.018 kb on + strand, within RR42_RS01570 at 354.019 kb on - strand, within RR42_RS01570 at 354.042 kb on + strand, within RR42_RS01570 at 354.183 kb on + strand, within RR42_RS01570 at 354.184 kb on - strand, within RR42_RS01570 at 354.184 kb on - strand, within RR42_RS01570 at 354.249 kb on + strand, within RR42_RS01570 at 354.250 kb on - strand, within RR42_RS01570 at 354.250 kb on - strand, within RR42_RS01570 at 354.250 kb on - strand, within RR42_RS01570 at 354.253 kb on + strand, within RR42_RS01570 at 354.253 kb on + strand, within RR42_RS01570 at 354.253 kb on + strand, within RR42_RS01570 at 354.254 kb on - strand, within RR42_RS01570 at 354.254 kb on - strand, within RR42_RS01570 at 354.254 kb on - strand, within RR42_RS01570 at 354.631 kb on + strand, within RR42_RS01570 at 354.942 kb on + strand, within RR42_RS01570 at 355.132 kb on - strand at 355.132 kb on - strand at 355.309 kb on + strand at 355.310 kb on - strand at 355.333 kb on - strand at 355.359 kb on + strand at 355.359 kb on + strand at 355.360 kb on - strand at 355.361 kb on + strand at 355.361 kb on + strand at 355.362 kb on - strand at 355.369 kb on - strand at 355.387 kb on - strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.546 kb on - strand at 355.587 kb on + strand at 355.587 kb on + strand at 355.587 kb on + strand at 355.588 kb on - strand at 355.588 kb on - strand at 355.590 kb on + strand at 355.590 kb on + strand at 355.591 kb on - strand at 355.857 kb on + strand, within RR42_RS01575 at 355.857 kb on + strand, within RR42_RS01575 at 355.857 kb on + strand, within RR42_RS01575 at 355.858 kb on - strand, within RR42_RS01575 at 356.024 kb on + strand, within RR42_RS01575 at 356.024 kb on + strand, within RR42_RS01575 at 356.025 kb on - strand, within RR42_RS01575 at 356.040 kb on + strand, within RR42_RS01575
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 352,685 + -0.2 352,685 + +1.3 352,686 - +0.5 352,736 + -1.4 352,737 - +0.9 352,754 + -1.1 352,765 - +1.5 352,769 - +0.2 352,821 + +1.5 352,821 + +0.3 352,822 - +1.5 352,901 - +2.0 352,968 + +0.0 352,968 + -0.0 352,969 - +0.6 352,969 - +1.6 352,969 - -0.6 352,969 - +1.5 352,996 + -1.4 352,997 - -0.2 353,034 + +0.6 353,035 + -0.0 353,035 + +1.9 353,035 + -0.9 353,035 + -0.2 353,035 + +1.0 353,035 + -0.4 353,035 + +0.9 353,035 + +1.8 353,035 + -2.5 353,036 - +0.6 353,036 - -1.1 353,036 - +0.2 353,036 - -0.7 353,036 - +0.5 353,036 - -0.6 353,036 - -0.3 353,036 - -0.3 353,144 + RR42_RS01565 0.27 -1.9 353,144 + RR42_RS01565 0.27 +0.6 353,144 + RR42_RS01565 0.27 +0.4 353,145 - RR42_RS01565 0.27 +0.6 353,145 - RR42_RS01565 0.27 -0.4 353,255 - RR42_RS01565 0.46 -1.4 353,371 + RR42_RS01565 0.66 +1.0 353,372 - RR42_RS01565 0.66 +0.4 353,372 - RR42_RS01565 0.66 +1.6 353,480 + RR42_RS01565 0.85 +1.5 353,543 + +0.7 353,544 - +1.3 353,544 - +0.9 353,544 - +0.6 353,759 + RR42_RS01570 0.10 +0.4 353,793 + RR42_RS01570 0.13 +0.8 353,794 - RR42_RS01570 0.13 +1.7 354,018 + RR42_RS01570 0.27 -1.8 354,018 + RR42_RS01570 0.27 +0.4 354,019 - RR42_RS01570 0.27 +0.9 354,042 + RR42_RS01570 0.29 -0.9 354,183 + RR42_RS01570 0.38 +1.5 354,184 - RR42_RS01570 0.38 -1.7 354,184 - RR42_RS01570 0.38 +0.0 354,249 + RR42_RS01570 0.43 -1.1 354,250 - RR42_RS01570 0.43 +2.1 354,250 - RR42_RS01570 0.43 +0.9 354,250 - RR42_RS01570 0.43 +0.4 354,253 + RR42_RS01570 0.43 -2.0 354,253 + RR42_RS01570 0.43 -0.3 354,253 + RR42_RS01570 0.43 -1.1 354,254 - RR42_RS01570 0.43 +1.6 354,254 - RR42_RS01570 0.43 +1.1 354,254 - RR42_RS01570 0.43 -0.1 354,631 + RR42_RS01570 0.68 -0.5 354,942 + RR42_RS01570 0.88 -0.7 355,132 - -0.1 355,132 - -1.2 355,309 + -0.5 355,310 - -0.2 355,333 - +1.5 355,359 + +2.5 355,359 + -2.5 355,360 - +1.5 355,361 + +0.4 355,361 + +0.3 355,362 - -1.7 355,369 - +0.0 355,387 - -0.7 355,475 + -0.4 355,475 + -0.7 355,475 + -2.4 355,475 + +0.8 355,475 + +0.9 355,475 + +0.7 355,475 + -0.3 355,475 + -3.0 355,475 + -0.1 355,476 - +0.2 355,476 - -0.3 355,476 - +0.0 355,476 - -0.4 355,476 - -1.5 355,546 - +0.6 355,587 + -1.5 355,587 + -2.2 355,587 + +0.5 355,588 - -0.1 355,588 - +0.7 355,590 + +0.2 355,590 + -0.6 355,591 - +0.4 355,857 + RR42_RS01575 0.29 +1.3 355,857 + RR42_RS01575 0.29 -0.2 355,857 + RR42_RS01575 0.29 -0.4 355,858 - RR42_RS01575 0.29 -2.6 356,024 + RR42_RS01575 0.41 -0.5 356,024 + RR42_RS01575 0.41 +0.5 356,025 - RR42_RS01575 0.41 +1.3 356,040 + RR42_RS01575 0.42 +0.8
Or see this region's nucleotide sequence