Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01555 and RR42_RS01560 are separated by 235 nucleotides RR42_RS01560 and RR42_RS01565 are separated by 362 nucleotides RR42_RS01565 and RR42_RS01570 are separated by 39 nucleotides
RR42_RS01555: RR42_RS01555 - aminopeptidase, at 349,421 to 350,611
_RS01555
RR42_RS01560: RR42_RS01560 - diguanylate cyclase, at 350,847 to 352,628
_RS01560
RR42_RS01565: RR42_RS01565 - hypothetical protein, at 352,991 to 353,563
_RS01565
RR42_RS01570: RR42_RS01570 - GntR family transcriptional regulator, at 353,603 to 355,120
_RS01570
Position (kb)
350
351
352
353 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 350.202 kb on - strand, within RR42_RS01555 at 350.204 kb on + strand, within RR42_RS01555 at 350.204 kb on + strand, within RR42_RS01555 at 350.205 kb on - strand, within RR42_RS01555 at 350.205 kb on - strand, within RR42_RS01555 at 350.205 kb on - strand, within RR42_RS01555 at 350.205 kb on - strand, within RR42_RS01555 at 350.205 kb on - strand, within RR42_RS01555 at 350.265 kb on - strand, within RR42_RS01555 at 350.381 kb on + strand, within RR42_RS01555 at 350.382 kb on - strand, within RR42_RS01555 at 350.382 kb on - strand, within RR42_RS01555 at 350.382 kb on - strand, within RR42_RS01555 at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.501 kb on + strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.502 kb on - strand at 350.508 kb on - strand at 350.664 kb on + strand at 350.665 kb on - strand at 350.680 kb on + strand at 350.681 kb on - strand at 351.099 kb on + strand, within RR42_RS01560 at 351.102 kb on + strand, within RR42_RS01560 at 351.157 kb on - strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.159 kb on + strand, within RR42_RS01560 at 351.160 kb on - strand, within RR42_RS01560 at 351.160 kb on - strand, within RR42_RS01560 at 351.160 kb on - strand, within RR42_RS01560 at 351.160 kb on - strand, within RR42_RS01560 at 351.703 kb on + strand, within RR42_RS01560 at 351.774 kb on + strand, within RR42_RS01560 at 351.774 kb on + strand, within RR42_RS01560 at 351.774 kb on + strand, within RR42_RS01560 at 351.774 kb on + strand, within RR42_RS01560 at 351.775 kb on - strand, within RR42_RS01560 at 351.775 kb on - strand, within RR42_RS01560 at 351.775 kb on - strand, within RR42_RS01560 at 351.775 kb on - strand, within RR42_RS01560 at 351.775 kb on - strand, within RR42_RS01560 at 352.081 kb on + strand, within RR42_RS01560 at 352.082 kb on - strand, within RR42_RS01560 at 352.082 kb on - strand, within RR42_RS01560 at 352.082 kb on - strand, within RR42_RS01560 at 352.082 kb on - strand, within RR42_RS01560 at 352.347 kb on + strand, within RR42_RS01560 at 352.348 kb on - strand, within RR42_RS01560 at 352.551 kb on + strand at 352.552 kb on - strand at 352.685 kb on + strand at 352.685 kb on + strand at 352.686 kb on - strand at 352.736 kb on + strand at 352.737 kb on - strand at 352.754 kb on + strand at 352.765 kb on - strand at 352.769 kb on - strand at 352.821 kb on + strand at 352.821 kb on + strand at 352.822 kb on - strand at 352.901 kb on - strand at 352.968 kb on + strand at 352.968 kb on + strand at 352.969 kb on - strand at 352.969 kb on - strand at 352.969 kb on - strand at 352.969 kb on - strand at 352.996 kb on + strand at 352.997 kb on - strand at 353.034 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.035 kb on + strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.036 kb on - strand at 353.144 kb on + strand, within RR42_RS01565 at 353.144 kb on + strand, within RR42_RS01565 at 353.144 kb on + strand, within RR42_RS01565 at 353.145 kb on - strand, within RR42_RS01565 at 353.145 kb on - strand, within RR42_RS01565 at 353.255 kb on - strand, within RR42_RS01565 at 353.371 kb on + strand, within RR42_RS01565 at 353.372 kb on - strand, within RR42_RS01565 at 353.372 kb on - strand, within RR42_RS01565 at 353.480 kb on + strand, within RR42_RS01565 at 353.543 kb on + strand at 353.544 kb on - strand at 353.544 kb on - strand at 353.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 350,202 - RR42_RS01555 0.66 -0.9 350,204 + RR42_RS01555 0.66 +0.5 350,204 + RR42_RS01555 0.66 +1.1 350,205 - RR42_RS01555 0.66 +0.9 350,205 - RR42_RS01555 0.66 -0.5 350,205 - RR42_RS01555 0.66 +0.3 350,205 - RR42_RS01555 0.66 -2.2 350,205 - RR42_RS01555 0.66 -0.6 350,265 - RR42_RS01555 0.71 +0.5 350,381 + RR42_RS01555 0.81 +0.0 350,382 - RR42_RS01555 0.81 -0.7 350,382 - RR42_RS01555 0.81 -2.3 350,382 - RR42_RS01555 0.81 +1.5 350,501 + -0.5 350,501 + -0.9 350,501 + +0.1 350,501 + -0.3 350,501 + +0.4 350,501 + +0.0 350,501 + +1.0 350,501 + -1.1 350,501 + -0.5 350,502 - +0.7 350,502 - +1.1 350,502 - +0.2 350,502 - -1.6 350,502 - -0.9 350,502 - +0.0 350,502 - -0.3 350,502 - -0.7 350,508 - -0.1 350,664 + -1.4 350,665 - +1.4 350,680 + -3.1 350,681 - -2.1 351,099 + RR42_RS01560 0.14 +0.1 351,102 + RR42_RS01560 0.14 -1.3 351,157 - RR42_RS01560 0.17 +0.3 351,159 + RR42_RS01560 0.18 -0.1 351,159 + RR42_RS01560 0.18 -0.4 351,159 + RR42_RS01560 0.18 +0.3 351,159 + RR42_RS01560 0.18 +0.8 351,159 + RR42_RS01560 0.18 +0.3 351,159 + RR42_RS01560 0.18 -1.0 351,159 + RR42_RS01560 0.18 +0.9 351,159 + RR42_RS01560 0.18 -0.6 351,159 + RR42_RS01560 0.18 -1.1 351,159 + RR42_RS01560 0.18 +0.8 351,159 + RR42_RS01560 0.18 +0.7 351,159 + RR42_RS01560 0.18 +1.1 351,159 + RR42_RS01560 0.18 +0.1 351,160 - RR42_RS01560 0.18 +0.6 351,160 - RR42_RS01560 0.18 -2.1 351,160 - RR42_RS01560 0.18 -0.5 351,160 - RR42_RS01560 0.18 +1.8 351,703 + RR42_RS01560 0.48 -1.5 351,774 + RR42_RS01560 0.52 -0.0 351,774 + RR42_RS01560 0.52 +0.6 351,774 + RR42_RS01560 0.52 +0.5 351,774 + RR42_RS01560 0.52 -0.3 351,775 - RR42_RS01560 0.52 -1.1 351,775 - RR42_RS01560 0.52 -0.7 351,775 - RR42_RS01560 0.52 -0.4 351,775 - RR42_RS01560 0.52 -0.4 351,775 - RR42_RS01560 0.52 -1.2 352,081 + RR42_RS01560 0.69 +0.7 352,082 - RR42_RS01560 0.69 -1.3 352,082 - RR42_RS01560 0.69 -0.6 352,082 - RR42_RS01560 0.69 -0.3 352,082 - RR42_RS01560 0.69 -2.6 352,347 + RR42_RS01560 0.84 -0.8 352,348 - RR42_RS01560 0.84 -1.5 352,551 + -1.4 352,552 - +0.7 352,685 + -0.2 352,685 + +1.3 352,686 - +0.5 352,736 + -1.4 352,737 - +0.9 352,754 + -1.1 352,765 - +1.5 352,769 - +0.2 352,821 + +1.5 352,821 + +0.3 352,822 - +1.5 352,901 - +2.0 352,968 + +0.0 352,968 + -0.0 352,969 - +0.6 352,969 - +1.6 352,969 - -0.6 352,969 - +1.5 352,996 + -1.4 352,997 - -0.2 353,034 + +0.6 353,035 + -0.0 353,035 + +1.9 353,035 + -0.9 353,035 + -0.2 353,035 + +1.0 353,035 + -0.4 353,035 + +0.9 353,035 + +1.8 353,035 + -2.5 353,036 - +0.6 353,036 - -1.1 353,036 - +0.2 353,036 - -0.7 353,036 - +0.5 353,036 - -0.6 353,036 - -0.3 353,036 - -0.3 353,144 + RR42_RS01565 0.27 -1.9 353,144 + RR42_RS01565 0.27 +0.6 353,144 + RR42_RS01565 0.27 +0.4 353,145 - RR42_RS01565 0.27 +0.6 353,145 - RR42_RS01565 0.27 -0.4 353,255 - RR42_RS01565 0.46 -1.4 353,371 + RR42_RS01565 0.66 +1.0 353,372 - RR42_RS01565 0.66 +0.4 353,372 - RR42_RS01565 0.66 +1.6 353,480 + RR42_RS01565 0.85 +1.5 353,543 + +0.7 353,544 - +1.3 353,544 - +0.9 353,544 - +0.6
Or see this region's nucleotide sequence