Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01540 and RR42_RS01545 are separated by 136 nucleotides RR42_RS01545 and RR42_RS01550 are separated by 113 nucleotides
RR42_RS01540: RR42_RS01540 - alpha/beta hydrolase, at 346,565 to 347,455
_RS01540
RR42_RS01545: RR42_RS01545 - TetR family transcriptional regulator, at 347,592 to 348,245
_RS01545
RR42_RS01550: RR42_RS01550 - ABC transporter substrate-binding protein, at 348,359 to 349,345
_RS01550
Position (kb) 347
348
349 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.794 kb on - strand, within RR42_RS01540 at 346.948 kb on + strand, within RR42_RS01540 at 346.970 kb on + strand, within RR42_RS01540 at 346.970 kb on + strand, within RR42_RS01540 at 346.970 kb on + strand, within RR42_RS01540 at 347.004 kb on - strand, within RR42_RS01540 at 347.006 kb on + strand, within RR42_RS01540 at 347.006 kb on + strand, within RR42_RS01540 at 347.007 kb on - strand, within RR42_RS01540 at 347.007 kb on - strand, within RR42_RS01540 at 347.007 kb on - strand, within RR42_RS01540 at 347.075 kb on + strand, within RR42_RS01540 at 347.076 kb on - strand, within RR42_RS01540 at 347.145 kb on - strand, within RR42_RS01540 at 347.381 kb on + strand at 347.382 kb on - strand at 347.453 kb on + strand at 347.471 kb on + strand at 347.471 kb on + strand at 347.472 kb on - strand at 347.478 kb on - strand at 347.484 kb on - strand at 347.495 kb on + strand at 347.542 kb on + strand at 347.543 kb on - strand at 347.636 kb on + strand at 347.781 kb on + strand, within RR42_RS01545 at 347.784 kb on + strand, within RR42_RS01545 at 347.784 kb on + strand, within RR42_RS01545 at 347.784 kb on + strand, within RR42_RS01545 at 347.785 kb on - strand, within RR42_RS01545 at 347.785 kb on - strand, within RR42_RS01545 at 347.917 kb on - strand, within RR42_RS01545 at 347.931 kb on + strand, within RR42_RS01545 at 347.931 kb on + strand, within RR42_RS01545 at 347.932 kb on - strand, within RR42_RS01545 at 347.964 kb on + strand, within RR42_RS01545 at 347.964 kb on + strand, within RR42_RS01545 at 348.020 kb on - strand, within RR42_RS01545 at 348.184 kb on - strand at 348.251 kb on - strand at 348.396 kb on + strand at 348.397 kb on - strand at 348.397 kb on - strand at 348.614 kb on + strand, within RR42_RS01550 at 348.614 kb on + strand, within RR42_RS01550 at 348.614 kb on + strand, within RR42_RS01550 at 348.614 kb on + strand, within RR42_RS01550 at 348.615 kb on - strand, within RR42_RS01550 at 348.615 kb on - strand, within RR42_RS01550 at 348.615 kb on - strand, within RR42_RS01550 at 348.615 kb on - strand, within RR42_RS01550 at 348.641 kb on + strand, within RR42_RS01550 at 348.707 kb on + strand, within RR42_RS01550 at 348.708 kb on - strand, within RR42_RS01550 at 348.708 kb on - strand, within RR42_RS01550 at 348.708 kb on - strand, within RR42_RS01550 at 348.765 kb on + strand, within RR42_RS01550 at 349.013 kb on + strand, within RR42_RS01550 at 349.013 kb on + strand, within RR42_RS01550 at 349.014 kb on - strand, within RR42_RS01550 at 349.130 kb on + strand, within RR42_RS01550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 346,691 - RR42_RS01540 0.14 +1.3 346,691 - RR42_RS01540 0.14 -2.7 346,691 - RR42_RS01540 0.14 +0.9 346,793 + RR42_RS01540 0.26 -0.7 346,793 + RR42_RS01540 0.26 -0.3 346,793 + RR42_RS01540 0.26 +0.3 346,794 - RR42_RS01540 0.26 -1.8 346,948 + RR42_RS01540 0.43 +0.6 346,970 + RR42_RS01540 0.45 -0.1 346,970 + RR42_RS01540 0.45 +1.6 346,970 + RR42_RS01540 0.45 -0.3 347,004 - RR42_RS01540 0.49 -0.6 347,006 + RR42_RS01540 0.49 +0.4 347,006 + RR42_RS01540 0.49 +0.0 347,007 - RR42_RS01540 0.50 -1.2 347,007 - RR42_RS01540 0.50 +0.6 347,007 - RR42_RS01540 0.50 +0.0 347,075 + RR42_RS01540 0.57 -2.3 347,076 - RR42_RS01540 0.57 +1.2 347,145 - RR42_RS01540 0.65 -0.3 347,381 + +2.4 347,382 - -1.1 347,453 + -0.1 347,471 + -0.5 347,471 + -1.8 347,472 - +0.9 347,478 - +2.1 347,484 - +1.3 347,495 + +0.6 347,542 + +2.6 347,543 - +0.0 347,636 + -0.8 347,781 + RR42_RS01545 0.29 +1.5 347,784 + RR42_RS01545 0.29 -0.2 347,784 + RR42_RS01545 0.29 -0.9 347,784 + RR42_RS01545 0.29 -2.1 347,785 - RR42_RS01545 0.30 -0.5 347,785 - RR42_RS01545 0.30 -3.5 347,917 - RR42_RS01545 0.50 -0.1 347,931 + RR42_RS01545 0.52 -0.5 347,931 + RR42_RS01545 0.52 -2.2 347,932 - RR42_RS01545 0.52 -1.1 347,964 + RR42_RS01545 0.57 -0.4 347,964 + RR42_RS01545 0.57 -0.6 348,020 - RR42_RS01545 0.65 +0.2 348,184 - -1.1 348,251 - +0.6 348,396 + -0.1 348,397 - -0.5 348,397 - +0.6 348,614 + RR42_RS01550 0.26 +1.3 348,614 + RR42_RS01550 0.26 -1.1 348,614 + RR42_RS01550 0.26 +0.5 348,614 + RR42_RS01550 0.26 -1.5 348,615 - RR42_RS01550 0.26 +2.4 348,615 - RR42_RS01550 0.26 +0.3 348,615 - RR42_RS01550 0.26 +0.5 348,615 - RR42_RS01550 0.26 +1.0 348,641 + RR42_RS01550 0.29 -0.5 348,707 + RR42_RS01550 0.35 -0.6 348,708 - RR42_RS01550 0.35 -1.1 348,708 - RR42_RS01550 0.35 -1.2 348,708 - RR42_RS01550 0.35 -2.2 348,765 + RR42_RS01550 0.41 +0.5 349,013 + RR42_RS01550 0.66 +1.1 349,013 + RR42_RS01550 0.66 -2.2 349,014 - RR42_RS01550 0.66 +1.0 349,130 + RR42_RS01550 0.78 +0.3
Or see this region's nucleotide sequence