Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01490 and RR42_RS01495 are separated by 17 nucleotides RR42_RS01495 and RR42_RS01500 are separated by 322 nucleotides RR42_RS01500 and RR42_RS01505 are separated by 149 nucleotides
RR42_RS01490: RR42_RS01490 - histidine kinase, at 336,120 to 337,553
_RS01490
RR42_RS01495: RR42_RS01495 - diguanylate cyclase, at 337,571 to 339,352
_RS01495
RR42_RS01500: RR42_RS01500 - hypothetical protein, at 339,675 to 340,094
_RS01500
RR42_RS01505: RR42_RS01505 - tRNA-Cys, at 340,244 to 340,318
_RS01505
Position (kb) 337
338
339
340 Strain fitness (log2 ratio) -2
-1
0
1
2 at 336.606 kb on + strand, within RR42_RS01490 at 336.606 kb on + strand, within RR42_RS01490 at 336.607 kb on - strand, within RR42_RS01490 at 336.607 kb on - strand, within RR42_RS01490 at 337.084 kb on + strand, within RR42_RS01490 at 337.084 kb on + strand, within RR42_RS01490 at 337.084 kb on + strand, within RR42_RS01490 at 337.170 kb on - strand, within RR42_RS01490 at 337.242 kb on + strand, within RR42_RS01490 at 337.255 kb on + strand, within RR42_RS01490 at 337.255 kb on + strand, within RR42_RS01490 at 337.256 kb on - strand, within RR42_RS01490 at 337.357 kb on + strand, within RR42_RS01490 at 337.358 kb on - strand, within RR42_RS01490 at 337.380 kb on + strand, within RR42_RS01490 at 337.381 kb on - strand, within RR42_RS01490 at 337.524 kb on + strand at 337.524 kb on + strand at 337.525 kb on - strand at 338.096 kb on + strand, within RR42_RS01495 at 338.097 kb on - strand, within RR42_RS01495 at 338.097 kb on - strand, within RR42_RS01495 at 338.100 kb on + strand, within RR42_RS01495 at 338.100 kb on + strand, within RR42_RS01495 at 338.100 kb on + strand, within RR42_RS01495 at 338.101 kb on - strand, within RR42_RS01495 at 338.410 kb on - strand, within RR42_RS01495 at 338.439 kb on + strand, within RR42_RS01495 at 338.439 kb on + strand, within RR42_RS01495 at 338.439 kb on + strand, within RR42_RS01495 at 338.439 kb on + strand, within RR42_RS01495 at 338.440 kb on - strand, within RR42_RS01495 at 338.440 kb on - strand, within RR42_RS01495 at 338.440 kb on - strand, within RR42_RS01495 at 338.440 kb on - strand, within RR42_RS01495 at 338.820 kb on + strand, within RR42_RS01495 at 338.858 kb on + strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.150 kb on + strand, within RR42_RS01495 at 339.275 kb on + strand at 339.275 kb on + strand at 339.275 kb on + strand at 339.336 kb on + strand at 339.337 kb on - strand at 339.337 kb on - strand at 339.340 kb on + strand at 339.341 kb on - strand at 339.482 kb on + strand at 339.482 kb on + strand at 339.482 kb on + strand at 339.519 kb on + strand at 339.562 kb on + strand at 339.563 kb on - strand at 339.633 kb on + strand at 339.642 kb on + strand at 339.912 kb on + strand, within RR42_RS01500 at 339.912 kb on + strand, within RR42_RS01500 at 339.913 kb on - strand, within RR42_RS01500 at 339.913 kb on - strand, within RR42_RS01500 at 340.161 kb on + strand at 340.162 kb on - strand at 340.162 kb on - strand at 340.285 kb on + strand, within RR42_RS01505 at 340.321 kb on - strand at 340.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 336,606 + RR42_RS01490 0.34 +0.3 336,606 + RR42_RS01490 0.34 -0.6 336,607 - RR42_RS01490 0.34 +0.9 336,607 - RR42_RS01490 0.34 -1.0 337,084 + RR42_RS01490 0.67 +0.4 337,084 + RR42_RS01490 0.67 -0.9 337,084 + RR42_RS01490 0.67 -1.4 337,170 - RR42_RS01490 0.73 +0.8 337,242 + RR42_RS01490 0.78 -1.3 337,255 + RR42_RS01490 0.79 +0.0 337,255 + RR42_RS01490 0.79 -1.2 337,256 - RR42_RS01490 0.79 -2.2 337,357 + RR42_RS01490 0.86 +0.7 337,358 - RR42_RS01490 0.86 -0.0 337,380 + RR42_RS01490 0.88 +2.0 337,381 - RR42_RS01490 0.88 -0.5 337,524 + +0.1 337,524 + +0.1 337,525 - +0.6 338,096 + RR42_RS01495 0.29 +2.1 338,097 - RR42_RS01495 0.30 -1.3 338,097 - RR42_RS01495 0.30 -0.4 338,100 + RR42_RS01495 0.30 +0.8 338,100 + RR42_RS01495 0.30 +1.2 338,100 + RR42_RS01495 0.30 +0.4 338,101 - RR42_RS01495 0.30 -0.4 338,410 - RR42_RS01495 0.47 +1.3 338,439 + RR42_RS01495 0.49 -0.7 338,439 + RR42_RS01495 0.49 -0.2 338,439 + RR42_RS01495 0.49 -0.9 338,439 + RR42_RS01495 0.49 -0.8 338,440 - RR42_RS01495 0.49 +0.1 338,440 - RR42_RS01495 0.49 +0.7 338,440 - RR42_RS01495 0.49 +0.8 338,440 - RR42_RS01495 0.49 +1.0 338,820 + RR42_RS01495 0.70 +0.1 338,858 + RR42_RS01495 0.72 +1.3 339,131 - RR42_RS01495 0.88 +1.3 339,131 - RR42_RS01495 0.88 -1.6 339,131 - RR42_RS01495 0.88 -0.2 339,131 - RR42_RS01495 0.88 -0.2 339,150 + RR42_RS01495 0.89 -1.9 339,275 + -0.7 339,275 + -1.2 339,275 + +0.2 339,336 + +0.3 339,337 - -0.2 339,337 - -0.1 339,340 + +0.4 339,341 - +1.1 339,482 + +1.4 339,482 + -0.0 339,482 + -0.3 339,519 + -0.2 339,562 + +1.4 339,563 - -0.2 339,633 + +1.1 339,642 + +1.4 339,912 + RR42_RS01500 0.56 -0.4 339,912 + RR42_RS01500 0.56 -1.6 339,913 - RR42_RS01500 0.57 +1.3 339,913 - RR42_RS01500 0.57 -0.0 340,161 + +1.0 340,162 - -0.1 340,162 - -0.8 340,285 + RR42_RS01505 0.55 +1.5 340,321 - -0.5 340,321 - -0.4
Or see this region's nucleotide sequence