Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01430 and RR42_RS01435 are separated by 96 nucleotides RR42_RS01435 and RR42_RS01440 are separated by 69 nucleotides RR42_RS01440 and RR42_RS01445 are separated by 155 nucleotides
RR42_RS01430: RR42_RS01430 - hypothetical protein, at 322,380 to 323,579
_RS01430
RR42_RS01435: RR42_RS01435 - short-chain dehydrogenase, at 323,676 to 324,422
_RS01435
RR42_RS01440: RR42_RS01440 - diguanylate cyclase, at 324,492 to 324,905
_RS01440
RR42_RS01445: RR42_RS01445 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 325,061 to 326,002
_RS01445
Position (kb) 324
325 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 323.590 kb on + strand at 323.590 kb on + strand at 323.590 kb on + strand at 323.987 kb on + strand, within RR42_RS01435 at 323.993 kb on + strand, within RR42_RS01435 at 323.993 kb on + strand, within RR42_RS01435 at 324.321 kb on + strand, within RR42_RS01435 at 324.903 kb on + strand at 324.904 kb on - strand at 325.106 kb on + strand at 325.106 kb on + strand at 325.107 kb on - strand at 325.107 kb on - strand at 325.107 kb on - strand at 325.202 kb on + strand, within RR42_RS01445 at 325.202 kb on + strand, within RR42_RS01445 at 325.202 kb on + strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.203 kb on - strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.343 kb on + strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.344 kb on - strand, within RR42_RS01445 at 325.368 kb on - strand, within RR42_RS01445 at 325.368 kb on - strand, within RR42_RS01445 at 325.445 kb on + strand, within RR42_RS01445 at 325.446 kb on - strand, within RR42_RS01445 at 325.446 kb on - strand, within RR42_RS01445 at 325.613 kb on + strand, within RR42_RS01445 at 325.641 kb on - strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.768 kb on + strand, within RR42_RS01445 at 325.769 kb on - strand, within RR42_RS01445
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 323,590 + +0.8 323,590 + -0.2 323,590 + +0.9 323,987 + RR42_RS01435 0.42 +0.4 323,993 + RR42_RS01435 0.42 -1.0 323,993 + RR42_RS01435 0.42 +0.7 324,321 + RR42_RS01435 0.86 +1.1 324,903 + -1.3 324,904 - -0.9 325,106 + -0.3 325,106 + -1.3 325,107 - -0.4 325,107 - -0.4 325,107 - -3.2 325,202 + RR42_RS01445 0.15 -1.8 325,202 + RR42_RS01445 0.15 -0.8 325,202 + RR42_RS01445 0.15 -0.4 325,203 - RR42_RS01445 0.15 -2.4 325,203 - RR42_RS01445 0.15 -0.5 325,203 - RR42_RS01445 0.15 -3.4 325,203 - RR42_RS01445 0.15 -3.9 325,203 - RR42_RS01445 0.15 -0.7 325,343 + RR42_RS01445 0.30 -0.7 325,343 + RR42_RS01445 0.30 +1.4 325,343 + RR42_RS01445 0.30 +0.7 325,343 + RR42_RS01445 0.30 -3.8 325,343 + RR42_RS01445 0.30 -0.6 325,344 - RR42_RS01445 0.30 -0.3 325,344 - RR42_RS01445 0.30 -0.9 325,344 - RR42_RS01445 0.30 +0.4 325,344 - RR42_RS01445 0.30 -1.2 325,344 - RR42_RS01445 0.30 +0.3 325,344 - RR42_RS01445 0.30 -1.4 325,344 - RR42_RS01445 0.30 -3.3 325,344 - RR42_RS01445 0.30 -2.4 325,368 - RR42_RS01445 0.33 +0.5 325,368 - RR42_RS01445 0.33 -1.7 325,445 + RR42_RS01445 0.41 -1.3 325,446 - RR42_RS01445 0.41 -0.7 325,446 - RR42_RS01445 0.41 -0.5 325,613 + RR42_RS01445 0.59 -2.1 325,641 - RR42_RS01445 0.62 +1.0 325,768 + RR42_RS01445 0.75 -2.1 325,768 + RR42_RS01445 0.75 -0.7 325,768 + RR42_RS01445 0.75 +0.2 325,768 + RR42_RS01445 0.75 -0.8 325,769 - RR42_RS01445 0.75 -2.3
Or see this region's nucleotide sequence