Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01265 and RR42_RS01270 are separated by 332 nucleotides RR42_RS01270 and RR42_RS01275 are separated by 48 nucleotides
RR42_RS01265: RR42_RS01265 - S-adenosylmethionine synthetase, at 285,827 to 286,990
_RS01265
RR42_RS01270: RR42_RS01270 - 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) hydroxylase, at 287,323 to 288,198
_RS01270
RR42_RS01275: RR42_RS01275 - aldehyde dehydrogenase, at 288,247 to 289,659
_RS01275
Position (kb) 287
288
289 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 286.988 kb on + strand at 286.988 kb on + strand at 287.132 kb on + strand at 287.341 kb on + strand at 287.341 kb on + strand at 287.343 kb on + strand at 287.343 kb on + strand at 287.344 kb on - strand at 287.344 kb on - strand at 287.572 kb on + strand, within RR42_RS01270 at 287.572 kb on + strand, within RR42_RS01270 at 287.572 kb on + strand, within RR42_RS01270 at 287.573 kb on - strand, within RR42_RS01270 at 287.573 kb on - strand, within RR42_RS01270 at 287.573 kb on - strand, within RR42_RS01270 at 287.600 kb on - strand, within RR42_RS01270 at 287.600 kb on - strand, within RR42_RS01270 at 287.647 kb on - strand, within RR42_RS01270 at 287.647 kb on - strand, within RR42_RS01270 at 287.647 kb on - strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.913 kb on + strand, within RR42_RS01270 at 287.914 kb on - strand, within RR42_RS01270 at 287.914 kb on - strand, within RR42_RS01270 at 287.938 kb on + strand, within RR42_RS01270 at 287.938 kb on + strand, within RR42_RS01270 at 287.939 kb on - strand, within RR42_RS01270 at 287.989 kb on + strand, within RR42_RS01270 at 287.990 kb on - strand, within RR42_RS01270 at 287.990 kb on - strand, within RR42_RS01270 at 288.047 kb on + strand, within RR42_RS01270 at 288.047 kb on + strand, within RR42_RS01270 at 288.048 kb on - strand, within RR42_RS01270 at 288.048 kb on - strand, within RR42_RS01270 at 288.106 kb on + strand, within RR42_RS01270 at 288.106 kb on + strand, within RR42_RS01270 at 288.107 kb on - strand, within RR42_RS01270 at 288.107 kb on - strand, within RR42_RS01270 at 288.107 kb on - strand, within RR42_RS01270 at 288.107 kb on - strand, within RR42_RS01270 at 288.109 kb on + strand, within RR42_RS01270 at 288.109 kb on + strand, within RR42_RS01270 at 288.109 kb on + strand, within RR42_RS01270 at 288.109 kb on + strand, within RR42_RS01270 at 288.110 kb on - strand, within RR42_RS01270 at 288.110 kb on - strand, within RR42_RS01270 at 288.110 kb on - strand, within RR42_RS01270 at 288.110 kb on - strand, within RR42_RS01270 at 288.138 kb on + strand at 288.138 kb on + strand at 288.138 kb on + strand at 288.138 kb on + strand at 288.138 kb on + strand at 288.139 kb on - strand at 288.139 kb on - strand at 288.139 kb on - strand at 288.139 kb on - strand at 288.139 kb on - strand at 288.139 kb on - strand at 288.383 kb on + strand at 288.383 kb on + strand at 288.384 kb on - strand at 288.384 kb on - strand at 288.384 kb on - strand at 288.384 kb on - strand at 288.527 kb on + strand, within RR42_RS01275 at 288.528 kb on - strand, within RR42_RS01275 at 288.528 kb on - strand, within RR42_RS01275 at 288.528 kb on - strand, within RR42_RS01275 at 288.560 kb on + strand, within RR42_RS01275 at 288.560 kb on + strand, within RR42_RS01275 at 288.560 kb on + strand, within RR42_RS01275 at 288.561 kb on - strand, within RR42_RS01275 at 288.561 kb on - strand, within RR42_RS01275 at 288.587 kb on + strand, within RR42_RS01275 at 288.902 kb on + strand, within RR42_RS01275 at 288.902 kb on + strand, within RR42_RS01275 at 288.902 kb on + strand, within RR42_RS01275 at 288.903 kb on - strand, within RR42_RS01275 at 288.903 kb on - strand, within RR42_RS01275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 286,988 + -0.5 286,988 + -0.5 287,132 + -0.2 287,341 + +2.6 287,341 + +1.8 287,343 + -1.0 287,343 + +1.8 287,344 - +0.9 287,344 - +1.6 287,572 + RR42_RS01270 0.28 +1.5 287,572 + RR42_RS01270 0.28 +1.3 287,572 + RR42_RS01270 0.28 +1.4 287,573 - RR42_RS01270 0.29 +0.2 287,573 - RR42_RS01270 0.29 +1.8 287,573 - RR42_RS01270 0.29 +1.1 287,600 - RR42_RS01270 0.32 +1.3 287,600 - RR42_RS01270 0.32 +1.6 287,647 - RR42_RS01270 0.37 +1.0 287,647 - RR42_RS01270 0.37 +4.2 287,647 - RR42_RS01270 0.37 +1.8 287,913 + RR42_RS01270 0.67 +3.6 287,913 + RR42_RS01270 0.67 +2.5 287,913 + RR42_RS01270 0.67 +1.2 287,913 + RR42_RS01270 0.67 +0.3 287,914 - RR42_RS01270 0.67 +1.0 287,914 - RR42_RS01270 0.67 +0.3 287,938 + RR42_RS01270 0.70 +1.6 287,938 + RR42_RS01270 0.70 +1.1 287,939 - RR42_RS01270 0.70 +0.4 287,989 + RR42_RS01270 0.76 +1.3 287,990 - RR42_RS01270 0.76 +2.8 287,990 - RR42_RS01270 0.76 +2.2 288,047 + RR42_RS01270 0.83 +1.4 288,047 + RR42_RS01270 0.83 -0.7 288,048 - RR42_RS01270 0.83 +1.4 288,048 - RR42_RS01270 0.83 +0.8 288,106 + RR42_RS01270 0.89 +0.3 288,106 + RR42_RS01270 0.89 +0.8 288,107 - RR42_RS01270 0.89 +0.7 288,107 - RR42_RS01270 0.89 +0.9 288,107 - RR42_RS01270 0.89 +1.0 288,107 - RR42_RS01270 0.89 +2.0 288,109 + RR42_RS01270 0.90 +1.1 288,109 + RR42_RS01270 0.90 +0.7 288,109 + RR42_RS01270 0.90 +0.6 288,109 + RR42_RS01270 0.90 -1.0 288,110 - RR42_RS01270 0.90 -0.3 288,110 - RR42_RS01270 0.90 +1.6 288,110 - RR42_RS01270 0.90 +0.0 288,110 - RR42_RS01270 0.90 +0.8 288,138 + +1.7 288,138 + +1.5 288,138 + +1.5 288,138 + +1.2 288,138 + +0.5 288,139 - +2.0 288,139 - +0.6 288,139 - +0.8 288,139 - +0.5 288,139 - +0.3 288,139 - +0.4 288,383 + -0.0 288,383 + -2.0 288,384 - -1.9 288,384 - +1.4 288,384 - +0.6 288,384 - -0.4 288,527 + RR42_RS01275 0.20 -0.1 288,528 - RR42_RS01275 0.20 +0.4 288,528 - RR42_RS01275 0.20 +0.0 288,528 - RR42_RS01275 0.20 +0.1 288,560 + RR42_RS01275 0.22 -0.3 288,560 + RR42_RS01275 0.22 -0.3 288,560 + RR42_RS01275 0.22 +0.3 288,561 - RR42_RS01275 0.22 +0.5 288,561 - RR42_RS01275 0.22 +0.5 288,587 + RR42_RS01275 0.24 +1.3 288,902 + RR42_RS01275 0.46 +2.0 288,902 + RR42_RS01275 0.46 -1.2 288,902 + RR42_RS01275 0.46 -1.5 288,903 - RR42_RS01275 0.46 +0.8 288,903 - RR42_RS01275 0.46 -0.7
Or see this region's nucleotide sequence