Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00975 and RR42_RS00980 are separated by 72 nucleotides RR42_RS00980 and RR42_RS00985 are separated by 58 nucleotides RR42_RS00985 and RR42_RS00990 are separated by 10 nucleotides RR42_RS00990 and RR42_RS00995 are separated by 158 nucleotides
RR42_RS00975: RR42_RS00975 - MBL fold metallo-hydrolase, at 230,222 to 231,154
_RS00975
RR42_RS00980: RR42_RS00980 - hypothetical protein, at 231,227 to 231,823
_RS00980
RR42_RS00985: RR42_RS00985 - acetyltransferase, at 231,882 to 232,496
_RS00985
RR42_RS00990: RR42_RS00990 - GntR family transcriptional regulator, at 232,507 to 233,244
_RS00990
RR42_RS00995: RR42_RS00995 - sodium:cation symporter, at 233,403 to 234,932
_RS00995
Position (kb)
231
232
233 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 230.900 kb on + strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.901 kb on - strand, within RR42_RS00975 at 231.141 kb on - strand at 231.141 kb on - strand at 231.392 kb on + strand, within RR42_RS00980 at 231.392 kb on + strand, within RR42_RS00980 at 231.392 kb on + strand, within RR42_RS00980 at 231.392 kb on + strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.470 kb on + strand, within RR42_RS00980 at 231.979 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.013 kb on + strand, within RR42_RS00985 at 232.014 kb on - strand, within RR42_RS00985 at 232.014 kb on - strand, within RR42_RS00985 at 232.077 kb on + strand, within RR42_RS00985 at 232.078 kb on - strand, within RR42_RS00985 at 232.078 kb on - strand, within RR42_RS00985 at 232.091 kb on - strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.117 kb on - strand, within RR42_RS00985 at 232.152 kb on + strand, within RR42_RS00985 at 232.153 kb on - strand, within RR42_RS00985 at 232.239 kb on + strand, within RR42_RS00985 at 232.239 kb on + strand, within RR42_RS00985 at 232.239 kb on + strand, within RR42_RS00985 at 232.239 kb on + strand, within RR42_RS00985 at 232.240 kb on - strand, within RR42_RS00985 at 232.240 kb on - strand, within RR42_RS00985 at 232.295 kb on + strand, within RR42_RS00985 at 232.295 kb on + strand, within RR42_RS00985 at 232.295 kb on + strand, within RR42_RS00985 at 232.295 kb on + strand, within RR42_RS00985 at 232.296 kb on - strand, within RR42_RS00985 at 232.296 kb on - strand, within RR42_RS00985 at 232.296 kb on - strand, within RR42_RS00985 at 232.296 kb on - strand, within RR42_RS00985 at 232.395 kb on + strand, within RR42_RS00985 at 232.396 kb on - strand, within RR42_RS00985 at 232.396 kb on - strand, within RR42_RS00985 at 232.416 kb on + strand, within RR42_RS00985 at 232.442 kb on + strand at 232.442 kb on + strand at 232.443 kb on - strand at 232.443 kb on - strand at 232.443 kb on - strand at 232.508 kb on + strand at 232.508 kb on + strand at 232.537 kb on - strand at 232.553 kb on + strand at 232.554 kb on - strand at 232.751 kb on + strand, within RR42_RS00990 at 232.751 kb on + strand, within RR42_RS00990 at 232.751 kb on + strand, within RR42_RS00990 at 232.751 kb on + strand, within RR42_RS00990 at 232.890 kb on + strand, within RR42_RS00990 at 232.977 kb on - strand, within RR42_RS00990 at 232.992 kb on + strand, within RR42_RS00990 at 232.993 kb on - strand, within RR42_RS00990 at 232.993 kb on - strand, within RR42_RS00990 at 232.993 kb on - strand, within RR42_RS00990 at 233.247 kb on - strand at 233.452 kb on + strand at 233.457 kb on + strand at 233.457 kb on + strand at 233.458 kb on - strand at 233.458 kb on - strand at 233.458 kb on - strand at 233.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 230,900 + RR42_RS00975 0.73 +1.7 230,900 + RR42_RS00975 0.73 -0.1 230,901 - RR42_RS00975 0.73 +0.7 231,141 - +0.0 231,141 - -0.1 231,392 + RR42_RS00980 0.28 -1.7 231,392 + RR42_RS00980 0.28 +0.9 231,392 + RR42_RS00980 0.28 +1.5 231,392 + RR42_RS00980 0.28 +0.5 231,393 - RR42_RS00980 0.28 -0.7 231,393 - RR42_RS00980 0.28 -0.4 231,393 - RR42_RS00980 0.28 -1.9 231,393 - RR42_RS00980 0.28 +3.1 231,470 + RR42_RS00980 0.41 -2.4 231,979 + RR42_RS00985 0.16 -0.6 232,011 + RR42_RS00985 0.21 -0.2 232,011 + RR42_RS00985 0.21 -0.8 232,011 + RR42_RS00985 0.21 -0.2 232,011 + RR42_RS00985 0.21 -1.0 232,013 + RR42_RS00985 0.21 -1.0 232,014 - RR42_RS00985 0.21 +0.6 232,014 - RR42_RS00985 0.21 +0.3 232,077 + RR42_RS00985 0.32 +0.9 232,078 - RR42_RS00985 0.32 +0.9 232,078 - RR42_RS00985 0.32 +1.9 232,091 - RR42_RS00985 0.34 +0.6 232,116 + RR42_RS00985 0.38 -0.2 232,116 + RR42_RS00985 0.38 +0.4 232,116 + RR42_RS00985 0.38 +0.4 232,116 + RR42_RS00985 0.38 -0.7 232,117 - RR42_RS00985 0.38 +0.4 232,152 + RR42_RS00985 0.44 +0.7 232,153 - RR42_RS00985 0.44 +1.1 232,239 + RR42_RS00985 0.58 -1.0 232,239 + RR42_RS00985 0.58 +0.1 232,239 + RR42_RS00985 0.58 +0.9 232,239 + RR42_RS00985 0.58 +0.6 232,240 - RR42_RS00985 0.58 -2.7 232,240 - RR42_RS00985 0.58 +1.6 232,295 + RR42_RS00985 0.67 -0.9 232,295 + RR42_RS00985 0.67 -2.6 232,295 + RR42_RS00985 0.67 +0.1 232,295 + RR42_RS00985 0.67 +1.1 232,296 - RR42_RS00985 0.67 -2.0 232,296 - RR42_RS00985 0.67 -0.8 232,296 - RR42_RS00985 0.67 +0.3 232,296 - RR42_RS00985 0.67 +0.7 232,395 + RR42_RS00985 0.83 -1.7 232,396 - RR42_RS00985 0.84 +2.1 232,396 - RR42_RS00985 0.84 +0.8 232,416 + RR42_RS00985 0.87 -0.1 232,442 + -1.8 232,442 + +0.1 232,443 - -1.2 232,443 - -0.7 232,443 - +1.0 232,508 + +1.5 232,508 + -0.5 232,537 - -1.0 232,553 + -0.4 232,554 - +0.9 232,751 + RR42_RS00990 0.33 -0.8 232,751 + RR42_RS00990 0.33 -1.2 232,751 + RR42_RS00990 0.33 +0.6 232,751 + RR42_RS00990 0.33 +0.3 232,890 + RR42_RS00990 0.52 -0.2 232,977 - RR42_RS00990 0.64 -1.2 232,992 + RR42_RS00990 0.66 -1.8 232,993 - RR42_RS00990 0.66 +1.1 232,993 - RR42_RS00990 0.66 +1.2 232,993 - RR42_RS00990 0.66 -0.1 233,247 - -0.4 233,452 + +1.2 233,457 + +0.5 233,457 + +0.2 233,458 - -1.0 233,458 - -0.4 233,458 - -1.7 233,458 - -1.0
Or see this region's nucleotide sequence