Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00955 and RR42_RS00960 are separated by 79 nucleotides RR42_RS00960 and RR42_RS00965 are separated by 60 nucleotides RR42_RS00965 and RR42_RS00970 are separated by 12 nucleotides
RR42_RS00955: RR42_RS00955 - hydroxymethylglutaryl-CoA lyase, at 227,673 to 228,608
_RS00955
RR42_RS00960: RR42_RS00960 - prolyl-tRNA synthetase, at 228,688 to 229,167
_RS00960
RR42_RS00965: RR42_RS00965 - Fe-S oxidoreductase, at 229,228 to 229,497
_RS00965
RR42_RS00970: RR42_RS00970 - DSBA oxidoreductase, at 229,510 to 230,199
_RS00970
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 227.693 kb on - strand at 227.805 kb on - strand, within RR42_RS00955 at 227.901 kb on + strand, within RR42_RS00955 at 227.921 kb on + strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.604 kb on + strand at 228.604 kb on + strand at 228.604 kb on + strand at 228.605 kb on - strand at 228.606 kb on + strand at 228.606 kb on + strand at 228.607 kb on - strand at 228.607 kb on - strand at 228.634 kb on - strand at 228.634 kb on - strand at 228.667 kb on - strand at 228.996 kb on + strand, within RR42_RS00960 at 228.996 kb on + strand, within RR42_RS00960 at 229.181 kb on - strand at 229.181 kb on - strand at 229.181 kb on - strand at 229.345 kb on + strand, within RR42_RS00965 at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.531 kb on + strand at 229.532 kb on - strand at 229.558 kb on + strand at 229.559 kb on - strand at 229.612 kb on + strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.920 kb on - strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.007 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 227,693 - -1.2 227,805 - RR42_RS00955 0.14 +0.1 227,901 + RR42_RS00955 0.24 -0.0 227,921 + RR42_RS00955 0.26 -1.4 227,922 - RR42_RS00955 0.27 -0.1 227,922 - RR42_RS00955 0.27 -1.1 228,158 - RR42_RS00955 0.52 -1.0 228,158 - RR42_RS00955 0.52 -0.7 228,307 - RR42_RS00955 0.68 +0.1 228,307 - RR42_RS00955 0.68 -0.7 228,441 + RR42_RS00955 0.82 -2.7 228,441 + RR42_RS00955 0.82 -2.7 228,442 - RR42_RS00955 0.82 -0.0 228,442 - RR42_RS00955 0.82 +0.3 228,604 + -0.0 228,604 + +0.5 228,604 + +1.7 228,605 - -0.3 228,606 + -0.5 228,606 + +0.1 228,607 - -0.7 228,607 - +1.5 228,634 - +1.6 228,634 - +0.7 228,667 - +0.5 228,996 + RR42_RS00960 0.64 +0.5 228,996 + RR42_RS00960 0.64 -0.6 229,181 - +0.7 229,181 - -1.5 229,181 - -0.8 229,345 + RR42_RS00965 0.43 -2.6 229,495 + +0.2 229,495 + -2.0 229,495 + -2.1 229,495 + -0.7 229,496 - -0.7 229,496 - -0.7 229,496 - -0.6 229,496 - +1.2 229,496 - +0.5 229,496 - +0.5 229,531 + +0.3 229,532 - -0.3 229,558 + -2.7 229,559 - -0.6 229,612 + RR42_RS00970 0.15 -0.1 229,613 - RR42_RS00970 0.15 -0.0 229,613 - RR42_RS00970 0.15 -1.0 229,920 - RR42_RS00970 0.59 +0.8 230,006 + RR42_RS00970 0.72 -1.5 230,006 + RR42_RS00970 0.72 -0.4 230,007 - RR42_RS00970 0.72 -0.4 230,011 - RR42_RS00970 0.73 -0.6 230,011 - RR42_RS00970 0.73 -2.8 230,011 - RR42_RS00970 0.73 +0.3
Or see this region's nucleotide sequence