Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00885 and RR42_RS00890 are separated by 84 nucleotides RR42_RS00890 and RR42_RS00895 are separated by 48 nucleotides RR42_RS00895 and RR42_RS00900 are separated by 119 nucleotides RR42_RS00900 and RR42_RS00905 overlap by 4 nucleotides
RR42_RS00885: RR42_RS00885 - acetyl-CoA acetyltransferase, at 213,807 to 215,003
_RS00885
RR42_RS00890: RR42_RS00890 - short-chain dehydrogenase, at 215,088 to 215,768
_RS00890
RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947
_RS00895
RR42_RS00900: RR42_RS00900 - 2-hydroxychromene-2-carboxylate isomerase, at 217,067 to 217,768
_RS00900
RR42_RS00905: RR42_RS00905 - haloacid dehalogenase, at 217,765 to 218,454
_RS00905
Position (kb) 215
216
217 Strain fitness (log2 ratio) -2
-1
0
1
2 at 215.067 kb on + strand at 215.305 kb on + strand, within RR42_RS00890 at 215.306 kb on - strand, within RR42_RS00890 at 215.306 kb on - strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.961 kb on + strand, within RR42_RS00895 at 215.985 kb on + strand, within RR42_RS00895 at 216.007 kb on - strand, within RR42_RS00895 at 216.253 kb on - strand, within RR42_RS00895 at 216.479 kb on + strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.504 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.895 kb on - strand at 216.922 kb on + strand at 216.923 kb on - strand at 216.923 kb on - strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.089 kb on - strand at 217.154 kb on + strand, within RR42_RS00900 at 217.250 kb on + strand, within RR42_RS00900 at 217.292 kb on - strand, within RR42_RS00900 at 217.299 kb on - strand, within RR42_RS00900 at 217.730 kb on - strand at 217.899 kb on + strand, within RR42_RS00905 at 217.900 kb on - strand, within RR42_RS00905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 215,067 + -2.2 215,305 + RR42_RS00890 0.32 +0.0 215,306 - RR42_RS00890 0.32 -0.2 215,306 - RR42_RS00890 0.32 +0.3 215,313 + RR42_RS00890 0.33 +0.1 215,313 + RR42_RS00890 0.33 -0.3 215,313 + RR42_RS00890 0.33 -0.8 215,742 + +1.1 215,742 + +0.7 215,742 + +1.7 215,742 + +0.3 215,742 + -1.2 215,742 + -1.5 215,743 - -0.5 215,743 - -1.2 215,743 - +0.0 215,743 - +0.9 215,743 - +2.7 215,961 + RR42_RS00895 0.13 +2.4 215,985 + RR42_RS00895 0.15 +0.1 216,007 - RR42_RS00895 0.17 -0.1 216,253 - RR42_RS00895 0.39 +0.4 216,479 + RR42_RS00895 0.59 +1.3 216,480 - RR42_RS00895 0.59 -0.3 216,480 - RR42_RS00895 0.59 -1.8 216,480 - RR42_RS00895 0.59 +2.6 216,504 + RR42_RS00895 0.61 -0.2 216,600 + RR42_RS00895 0.69 +0.2 216,600 + RR42_RS00895 0.69 -1.2 216,600 + RR42_RS00895 0.69 +0.2 216,774 + RR42_RS00895 0.85 +0.1 216,774 + RR42_RS00895 0.85 -1.7 216,775 - RR42_RS00895 0.85 +0.7 216,775 - RR42_RS00895 0.85 +1.3 216,895 - -1.7 216,922 + +0.8 216,923 - -0.3 216,923 - +0.0 217,088 + -1.5 217,088 + +0.5 217,088 + -0.5 217,089 - -2.4 217,154 + RR42_RS00900 0.12 +0.2 217,250 + RR42_RS00900 0.26 +0.6 217,292 - RR42_RS00900 0.32 +1.3 217,299 - RR42_RS00900 0.33 +1.0 217,730 - -1.1 217,899 + RR42_RS00905 0.19 +0.2 217,900 - RR42_RS00905 0.20 +1.5
Or see this region's nucleotide sequence