Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00775 and RR42_RS00780 are separated by 238 nucleotides RR42_RS00780 and RR42_RS00785 are separated by 316 nucleotides RR42_RS00785 and RR42_RS00790 are separated by 109 nucleotides RR42_RS00790 and RR42_RS00795 are separated by 105 nucleotides RR42_RS00795 and RR42_RS00800 are separated by 107 nucleotides
RR42_RS00775: RR42_RS00775 - methionine synthase, at 192,720 to 195,467
_RS00775
RR42_RS00780: RR42_RS00780 - hypothetical protein, at 195,706 to 196,065
_RS00780
RR42_RS00785: RR42_RS00785 - LuxR family transcriptional regulator, at 196,382 to 197,014
_RS00785
RR42_RS00790: RR42_RS00790 - chemotaxis protein CheY, at 197,124 to 197,531
_RS00790
RR42_RS00795: RR42_RS00795 - bacteriolytic lipoprotein entericidin AB, at 197,637 to 197,780
_RS00795
RR42_RS00800: RR42_RS00800 - histidine kinase, at 197,888 to 199,342
_RS00800
Position (kb) 196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 195.404 kb on + strand at 195.580 kb on + strand at 195.581 kb on - strand at 195.587 kb on + strand at 195.587 kb on + strand at 195.587 kb on + strand at 195.588 kb on - strand at 195.597 kb on + strand at 195.597 kb on + strand at 195.597 kb on + strand at 195.598 kb on - strand at 195.598 kb on - strand at 195.598 kb on - strand at 195.598 kb on - strand at 195.633 kb on - strand at 195.693 kb on + strand at 196.063 kb on + strand at 196.063 kb on + strand at 196.064 kb on - strand at 196.188 kb on + strand at 196.188 kb on + strand at 196.189 kb on - strand at 196.189 kb on - strand at 196.189 kb on - strand at 196.189 kb on - strand at 196.189 kb on - strand at 196.192 kb on + strand at 196.193 kb on - strand at 196.322 kb on + strand at 196.323 kb on - strand at 196.441 kb on + strand at 196.544 kb on - strand, within RR42_RS00785 at 196.683 kb on - strand, within RR42_RS00785 at 196.751 kb on + strand, within RR42_RS00785 at 196.751 kb on + strand, within RR42_RS00785 at 196.929 kb on - strand, within RR42_RS00785 at 196.929 kb on - strand, within RR42_RS00785 at 196.982 kb on + strand at 196.982 kb on + strand at 196.982 kb on + strand at 196.983 kb on - strand at 196.986 kb on - strand at 197.047 kb on - strand at 197.101 kb on + strand at 197.174 kb on - strand, within RR42_RS00790 at 197.201 kb on + strand, within RR42_RS00790 at 197.202 kb on - strand, within RR42_RS00790 at 197.299 kb on - strand, within RR42_RS00790 at 197.299 kb on - strand, within RR42_RS00790 at 197.397 kb on + strand, within RR42_RS00790 at 197.397 kb on + strand, within RR42_RS00790 at 197.398 kb on - strand, within RR42_RS00790 at 197.398 kb on - strand, within RR42_RS00790 at 197.398 kb on - strand, within RR42_RS00790 at 197.420 kb on + strand, within RR42_RS00790 at 197.421 kb on - strand, within RR42_RS00790 at 197.421 kb on - strand, within RR42_RS00790 at 197.424 kb on + strand, within RR42_RS00790 at 197.511 kb on + strand at 197.512 kb on - strand at 197.546 kb on + strand at 197.547 kb on - strand at 197.565 kb on - strand at 197.565 kb on - strand at 197.568 kb on + strand at 197.568 kb on + strand at 197.569 kb on - strand at 197.573 kb on + strand at 197.574 kb on - strand at 197.574 kb on - strand at 197.778 kb on + strand at 197.778 kb on + strand at 197.778 kb on + strand at 197.778 kb on + strand at 197.778 kb on + strand at 197.779 kb on - strand at 197.779 kb on - strand at 197.779 kb on - strand at 197.779 kb on - strand at 197.825 kb on - strand at 197.889 kb on + strand at 197.889 kb on + strand at 197.890 kb on - strand at 197.890 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 195,404 + +0.3 195,580 + +0.7 195,581 - -1.0 195,587 + -2.6 195,587 + -0.3 195,587 + +0.6 195,588 - -3.0 195,597 + -0.0 195,597 + +0.4 195,597 + -0.9 195,598 - +1.3 195,598 - -1.0 195,598 - -0.4 195,598 - -1.8 195,633 - -0.0 195,693 + -1.5 196,063 + -0.5 196,063 + +0.2 196,064 - +1.7 196,188 + +1.7 196,188 + +0.5 196,189 - +0.2 196,189 - +0.7 196,189 - +1.1 196,189 - -1.7 196,189 - +2.4 196,192 + +0.3 196,193 - +0.5 196,322 + -0.4 196,323 - -1.3 196,441 + -0.2 196,544 - RR42_RS00785 0.26 -2.0 196,683 - RR42_RS00785 0.48 -2.7 196,751 + RR42_RS00785 0.58 -0.8 196,751 + RR42_RS00785 0.58 -0.8 196,929 - RR42_RS00785 0.86 +0.3 196,929 - RR42_RS00785 0.86 +0.0 196,982 + -1.8 196,982 + +0.3 196,982 + -1.2 196,983 - +1.1 196,986 - +0.7 197,047 - -1.0 197,101 + -0.7 197,174 - RR42_RS00790 0.12 -0.1 197,201 + RR42_RS00790 0.19 +0.1 197,202 - RR42_RS00790 0.19 -1.1 197,299 - RR42_RS00790 0.43 +0.6 197,299 - RR42_RS00790 0.43 -1.1 197,397 + RR42_RS00790 0.67 -1.1 197,397 + RR42_RS00790 0.67 -1.9 197,398 - RR42_RS00790 0.67 -0.5 197,398 - RR42_RS00790 0.67 -0.8 197,398 - RR42_RS00790 0.67 -0.4 197,420 + RR42_RS00790 0.73 -0.8 197,421 - RR42_RS00790 0.73 -0.9 197,421 - RR42_RS00790 0.73 -0.3 197,424 + RR42_RS00790 0.74 +0.4 197,511 + +1.6 197,512 - -2.9 197,546 + -0.0 197,547 - +0.6 197,565 - +1.4 197,565 - -2.0 197,568 + -1.7 197,568 + +0.3 197,569 - -1.5 197,573 + -1.1 197,574 - -0.7 197,574 - +0.1 197,778 + +0.2 197,778 + +0.1 197,778 + -0.2 197,778 + +0.0 197,778 + -0.8 197,779 - -0.7 197,779 - +0.7 197,779 - -0.5 197,779 - -0.1 197,825 - +0.8 197,889 + +0.5 197,889 + -0.0 197,890 - -1.5 197,890 - -1.0
Or see this region's nucleotide sequence