Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00620 and RR42_RS00625 are separated by 230 nucleotides RR42_RS00625 and RR42_RS00630 are separated by 177 nucleotides RR42_RS00630 and RR42_RS00635 are separated by 43 nucleotides
RR42_RS00620: RR42_RS00620 - XRE family transcriptional regulator, at 162,407 to 163,366
_RS00620
RR42_RS00625: RR42_RS00625 - hypothetical protein, at 163,597 to 163,956
_RS00625
RR42_RS00630: RR42_RS00630 - lipoate--protein ligase, at 164,134 to 164,865
_RS00630
RR42_RS00635: RR42_RS00635 - lipoyl synthase, at 164,909 to 165,904
_RS00635
Position (kb) 163
164 Strain fitness (log2 ratio) -2
-1
0
1
2 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 163.413 kb on + strand at 163.444 kb on + strand at 163.445 kb on - strand at 163.445 kb on - strand at 163.445 kb on - strand at 163.490 kb on + strand at 163.509 kb on + strand at 163.742 kb on - strand, within RR42_RS00625 at 164.122 kb on + strand at 164.145 kb on - strand at 164.145 kb on - strand at 164.863 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 162,849 + RR42_RS00620 0.46 -0.6 162,849 + RR42_RS00620 0.46 -1.5 162,849 + RR42_RS00620 0.46 +1.5 162,849 + RR42_RS00620 0.46 -1.0 162,850 - RR42_RS00620 0.46 -0.6 162,850 - RR42_RS00620 0.46 +0.1 162,850 - RR42_RS00620 0.46 -1.8 162,850 - RR42_RS00620 0.46 +1.7 162,850 - RR42_RS00620 0.46 -1.6 163,413 + +0.4 163,444 + +0.8 163,445 - +2.7 163,445 - +0.4 163,445 - -0.6 163,490 + +2.0 163,509 + -0.2 163,742 - RR42_RS00625 0.40 -1.3 164,122 + +2.2 164,145 - +0.5 164,145 - +0.8 164,863 + +1.0
Or see this region's nucleotide sequence