Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00590 and RR42_RS00595 overlap by 4 nucleotides RR42_RS00595 and RR42_RS00600 overlap by 4 nucleotides RR42_RS00600 and RR42_RS00605 are separated by 106 nucleotides
RR42_RS00590: RR42_RS00590 - glutamyl-tRNA amidotransferase, at 155,908 to 157,413
_RS00590
RR42_RS00595: RR42_RS00595 - lipoprotein, at 157,410 to 157,877
_RS00595
RR42_RS00600: RR42_RS00600 - DeoR family transcriptional regulator, at 157,874 to 158,845
_RS00600
RR42_RS00605: RR42_RS00605 - glutamyl-tRNA amidotransferase, at 158,952 to 160,412
_RS00605
Position (kb) 157
158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 157.703 kb on + strand, within RR42_RS00595 at 157.703 kb on + strand, within RR42_RS00595 at 157.815 kb on + strand, within RR42_RS00595 at 157.815 kb on + strand, within RR42_RS00595 at 157.815 kb on + strand, within RR42_RS00595 at 158.678 kb on + strand, within RR42_RS00600 at 158.855 kb on + strand at 158.861 kb on + strand at 158.861 kb on + strand at 158.861 kb on + strand at 158.865 kb on + strand at 158.865 kb on + strand at 158.865 kb on + strand at 158.905 kb on + strand at 158.905 kb on + strand at 158.905 kb on + strand at 158.916 kb on + strand at 158.916 kb on + strand at 158.916 kb on + strand at 158.926 kb on + strand at 158.926 kb on + strand at 158.926 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 157,703 + RR42_RS00595 0.63 +0.4 157,703 + RR42_RS00595 0.63 -0.6 157,815 + RR42_RS00595 0.87 +0.1 157,815 + RR42_RS00595 0.87 -0.7 157,815 + RR42_RS00595 0.87 +0.3 158,678 + RR42_RS00600 0.83 -1.2 158,855 + -2.2 158,861 + +0.2 158,861 + -0.7 158,861 + +0.4 158,865 + +0.2 158,865 + -0.6 158,865 + -2.3 158,905 + +0.4 158,905 + +0.1 158,905 + +0.6 158,916 + -2.9 158,916 + +0.3 158,916 + -0.0 158,926 + -0.3 158,926 + -0.9 158,926 + -0.4
Or see this region's nucleotide sequence