Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00505 and RR42_RS00510 overlap by 34 nucleotides RR42_RS00510 and RR42_RS00515 are separated by 229 nucleotides
RR42_RS00505: RR42_RS00505 - histidine kinase, at 131,990 to 134,026
_RS00505
RR42_RS00510: RR42_RS00510 - transcriptional regulator, at 133,993 to 134,652
_RS00510
RR42_RS00515: RR42_RS00515 - phosphonate monoester hydrolase, at 134,882 to 136,441
_RS00515
Position (kb) 133
134
135 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 133.091 kb on + strand, within RR42_RS00505 at 133.092 kb on - strand, within RR42_RS00505 at 133.146 kb on - strand, within RR42_RS00505 at 133.703 kb on + strand, within RR42_RS00505 at 133.703 kb on + strand, within RR42_RS00505 at 133.703 kb on + strand, within RR42_RS00505 at 133.703 kb on + strand, within RR42_RS00505 at 133.704 kb on - strand, within RR42_RS00505 at 133.704 kb on - strand, within RR42_RS00505 at 133.927 kb on + strand at 133.928 kb on - strand at 134.022 kb on + strand at 134.022 kb on + strand at 134.022 kb on + strand at 134.022 kb on + strand at 134.022 kb on + strand at 134.022 kb on + strand at 134.022 kb on + strand at 134.023 kb on - strand at 134.023 kb on - strand at 134.024 kb on + strand at 134.025 kb on - strand at 134.025 kb on - strand at 134.025 kb on - strand at 134.127 kb on - strand, within RR42_RS00510 at 134.363 kb on + strand, within RR42_RS00510 at 134.363 kb on + strand, within RR42_RS00510 at 134.392 kb on + strand, within RR42_RS00510 at 134.393 kb on - strand, within RR42_RS00510 at 134.602 kb on + strand at 134.602 kb on + strand at 134.759 kb on + strand at 134.771 kb on + strand at 134.772 kb on - strand at 134.796 kb on - strand at 134.799 kb on - strand at 134.916 kb on + strand at 134.917 kb on - strand at 134.917 kb on - strand at 135.111 kb on + strand, within RR42_RS00515 at 135.111 kb on + strand, within RR42_RS00515 at 135.111 kb on + strand, within RR42_RS00515 at 135.112 kb on - strand, within RR42_RS00515 at 135.112 kb on - strand, within RR42_RS00515 at 135.195 kb on + strand, within RR42_RS00515 at 135.196 kb on - strand, within RR42_RS00515 at 135.396 kb on + strand, within RR42_RS00515 at 135.397 kb on - strand, within RR42_RS00515 at 135.429 kb on + strand, within RR42_RS00515 at 135.429 kb on + strand, within RR42_RS00515 at 135.430 kb on - strand, within RR42_RS00515 at 135.583 kb on - strand, within RR42_RS00515
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 133,091 + RR42_RS00505 0.54 +0.9 133,092 - RR42_RS00505 0.54 +1.3 133,146 - RR42_RS00505 0.57 -0.6 133,703 + RR42_RS00505 0.84 -2.5 133,703 + RR42_RS00505 0.84 -0.8 133,703 + RR42_RS00505 0.84 -0.5 133,703 + RR42_RS00505 0.84 -0.2 133,704 - RR42_RS00505 0.84 +0.9 133,704 - RR42_RS00505 0.84 +1.4 133,927 + -1.0 133,928 - +0.4 134,022 + +1.0 134,022 + -0.4 134,022 + -0.6 134,022 + +1.1 134,022 + +0.6 134,022 + +1.3 134,022 + +0.1 134,023 - +0.6 134,023 - -2.6 134,024 + +0.7 134,025 - +0.4 134,025 - +0.1 134,025 - -0.3 134,127 - RR42_RS00510 0.20 +1.5 134,363 + RR42_RS00510 0.56 -1.1 134,363 + RR42_RS00510 0.56 -0.2 134,392 + RR42_RS00510 0.60 +1.9 134,393 - RR42_RS00510 0.61 -0.2 134,602 + -1.2 134,602 + +0.4 134,759 + -1.2 134,771 + -2.0 134,772 - -0.6 134,796 - +0.0 134,799 - +0.2 134,916 + -2.2 134,917 - -0.2 134,917 - -0.4 135,111 + RR42_RS00515 0.15 -0.9 135,111 + RR42_RS00515 0.15 -0.2 135,111 + RR42_RS00515 0.15 +0.1 135,112 - RR42_RS00515 0.15 -2.2 135,112 - RR42_RS00515 0.15 -0.6 135,195 + RR42_RS00515 0.20 -3.2 135,196 - RR42_RS00515 0.20 +0.2 135,396 + RR42_RS00515 0.33 -1.5 135,397 - RR42_RS00515 0.33 +0.0 135,429 + RR42_RS00515 0.35 -1.0 135,429 + RR42_RS00515 0.35 -0.1 135,430 - RR42_RS00515 0.35 +0.4 135,583 - RR42_RS00515 0.45 -1.1
Or see this region's nucleotide sequence