Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00415 and RR42_RS00420 are separated by 128 nucleotides RR42_RS00420 and RR42_RS00425 are separated by 126 nucleotides RR42_RS00425 and RR42_RS00430 are separated by 17 nucleotides RR42_RS00430 and RR42_RS00435 are separated by 232 nucleotides
RR42_RS00415: RR42_RS00415 - ABC transporter permease, at 110,626 to 111,465
_RS00415
RR42_RS00420: RR42_RS00420 - hypothetical protein, at 111,594 to 111,884
_RS00420
RR42_RS00425: RR42_RS00425 - ABC transporter substrate-binding protein, at 112,011 to 113,024
_RS00425
RR42_RS00430: RR42_RS00430 - histidine kinase, at 113,042 to 113,734
_RS00430
RR42_RS00435: RR42_RS00435 - transcriptional regulator, at 113,967 to 114,665
_RS00435
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 111.227 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.362 kb on + strand, within RR42_RS00415 at 111.363 kb on - strand, within RR42_RS00415 at 111.496 kb on - strand at 111.616 kb on + strand at 111.616 kb on + strand at 111.796 kb on + strand, within RR42_RS00420 at 111.797 kb on - strand, within RR42_RS00420 at 111.797 kb on - strand, within RR42_RS00420 at 111.807 kb on + strand, within RR42_RS00420 at 111.807 kb on + strand, within RR42_RS00420 at 111.808 kb on - strand, within RR42_RS00420 at 111.883 kb on - strand at 112.129 kb on - strand, within RR42_RS00425 at 112.374 kb on + strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.599 kb on + strand, within RR42_RS00425 at 113.082 kb on + strand at 113.082 kb on + strand at 113.082 kb on + strand at 113.082 kb on + strand at 113.082 kb on + strand at 113.083 kb on - strand at 113.083 kb on - strand at 113.083 kb on - strand at 113.332 kb on - strand, within RR42_RS00430 at 113.601 kb on + strand, within RR42_RS00430 at 113.602 kb on - strand, within RR42_RS00430 at 113.773 kb on - strand at 113.862 kb on + strand at 113.863 kb on - strand at 113.863 kb on - strand at 113.917 kb on + strand at 113.966 kb on + strand at 113.966 kb on + strand at 113.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 111,227 - RR42_RS00415 0.72 +0.4 111,227 - RR42_RS00415 0.72 +0.8 111,262 + RR42_RS00415 0.76 +3.4 111,262 + RR42_RS00415 0.76 -1.5 111,262 + RR42_RS00415 0.76 +0.6 111,263 - RR42_RS00415 0.76 -0.4 111,263 - RR42_RS00415 0.76 +0.4 111,263 - RR42_RS00415 0.76 +0.4 111,362 + RR42_RS00415 0.88 -2.3 111,363 - RR42_RS00415 0.88 -0.2 111,496 - +0.8 111,616 + -0.6 111,616 + -1.6 111,796 + RR42_RS00420 0.69 -0.2 111,797 - RR42_RS00420 0.70 -1.0 111,797 - RR42_RS00420 0.70 -1.2 111,807 + RR42_RS00420 0.73 +1.0 111,807 + RR42_RS00420 0.73 +0.9 111,808 - RR42_RS00420 0.74 -0.1 111,883 - -1.3 112,129 - RR42_RS00425 0.12 -0.3 112,374 + RR42_RS00425 0.36 -1.2 112,375 - RR42_RS00425 0.36 +1.4 112,375 - RR42_RS00425 0.36 -0.2 112,375 - RR42_RS00425 0.36 -0.0 112,375 - RR42_RS00425 0.36 -2.9 112,599 + RR42_RS00425 0.58 -3.6 113,082 + -1.6 113,082 + -1.8 113,082 + +0.7 113,082 + -2.8 113,082 + -1.0 113,083 - +0.8 113,083 - +0.4 113,083 - +0.3 113,332 - RR42_RS00430 0.42 +0.8 113,601 + RR42_RS00430 0.81 -0.1 113,602 - RR42_RS00430 0.81 +0.4 113,773 - -2.3 113,862 + -0.6 113,863 - -2.2 113,863 - -1.5 113,917 + +1.4 113,966 + -0.2 113,966 + -2.4 113,966 + +0.4
Or see this region's nucleotide sequence