Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00375 and RR42_RS00380 are separated by 163 nucleotides RR42_RS00380 and RR42_RS00385 overlap by 4 nucleotides
RR42_RS00375: RR42_RS00375 - MFS transporter, at 101,070 to 102,368
_RS00375
RR42_RS00380: RR42_RS00380 - GntR family transcriptional regulator, at 102,532 to 103,278
_RS00380
RR42_RS00385: RR42_RS00385 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 103,275 to 104,117
_RS00385
Position (kb) 102
103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 101.573 kb on - strand, within RR42_RS00375 at 101.599 kb on + strand, within RR42_RS00375 at 101.653 kb on + strand, within RR42_RS00375 at 101.653 kb on + strand, within RR42_RS00375 at 101.654 kb on - strand, within RR42_RS00375 at 101.729 kb on + strand, within RR42_RS00375 at 101.731 kb on + strand, within RR42_RS00375 at 101.731 kb on + strand, within RR42_RS00375 at 101.732 kb on - strand, within RR42_RS00375 at 101.785 kb on + strand, within RR42_RS00375 at 101.786 kb on - strand, within RR42_RS00375 at 101.786 kb on - strand, within RR42_RS00375 at 101.788 kb on + strand, within RR42_RS00375 at 101.788 kb on + strand, within RR42_RS00375 at 101.788 kb on + strand, within RR42_RS00375 at 102.124 kb on + strand, within RR42_RS00375 at 102.124 kb on + strand, within RR42_RS00375 at 102.124 kb on + strand, within RR42_RS00375 at 102.125 kb on - strand, within RR42_RS00375 at 102.166 kb on + strand, within RR42_RS00375 at 102.167 kb on - strand, within RR42_RS00375 at 102.271 kb on + strand at 102.272 kb on - strand at 102.272 kb on - strand at 102.272 kb on - strand at 102.574 kb on - strand at 102.574 kb on - strand at 102.772 kb on + strand, within RR42_RS00380 at 102.773 kb on - strand, within RR42_RS00380 at 102.958 kb on + strand, within RR42_RS00380 at 102.958 kb on + strand, within RR42_RS00380 at 102.959 kb on - strand, within RR42_RS00380 at 102.959 kb on - strand, within RR42_RS00380 at 102.959 kb on - strand, within RR42_RS00380 at 103.030 kb on + strand, within RR42_RS00380 at 103.030 kb on + strand, within RR42_RS00380 at 103.030 kb on + strand, within RR42_RS00380 at 103.030 kb on + strand, within RR42_RS00380 at 103.030 kb on + strand, within RR42_RS00380 at 103.030 kb on + strand, within RR42_RS00380 at 103.031 kb on - strand, within RR42_RS00380 at 103.031 kb on - strand, within RR42_RS00380 at 103.031 kb on - strand, within RR42_RS00380 at 103.084 kb on - strand, within RR42_RS00380 at 103.282 kb on + strand at 103.282 kb on + strand at 103.282 kb on + strand at 103.283 kb on - strand at 103.283 kb on - strand at 103.283 kb on - strand at 103.285 kb on + strand at 103.285 kb on + strand at 103.286 kb on - strand at 103.286 kb on - strand at 103.286 kb on - strand at 103.286 kb on - strand at 103.295 kb on + strand at 103.510 kb on - strand, within RR42_RS00385 at 103.510 kb on - strand, within RR42_RS00385 at 103.947 kb on + strand, within RR42_RS00385 at 104.109 kb on + strand at 104.115 kb on + strand at 104.115 kb on + strand at 104.116 kb on - strand at 104.199 kb on + strand at 104.199 kb on + strand at 104.203 kb on + strand at 104.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 101,573 - RR42_RS00375 0.39 +0.2 101,599 + RR42_RS00375 0.41 -2.1 101,653 + RR42_RS00375 0.45 +1.4 101,653 + RR42_RS00375 0.45 -0.2 101,654 - RR42_RS00375 0.45 +1.7 101,729 + RR42_RS00375 0.51 +0.4 101,731 + RR42_RS00375 0.51 -1.2 101,731 + RR42_RS00375 0.51 +0.0 101,732 - RR42_RS00375 0.51 -1.0 101,785 + RR42_RS00375 0.55 -1.0 101,786 - RR42_RS00375 0.55 -0.1 101,786 - RR42_RS00375 0.55 +0.5 101,788 + RR42_RS00375 0.55 +0.5 101,788 + RR42_RS00375 0.55 -0.7 101,788 + RR42_RS00375 0.55 +0.7 102,124 + RR42_RS00375 0.81 +0.8 102,124 + RR42_RS00375 0.81 -0.3 102,124 + RR42_RS00375 0.81 -0.2 102,125 - RR42_RS00375 0.81 +1.0 102,166 + RR42_RS00375 0.84 -1.8 102,167 - RR42_RS00375 0.84 -0.2 102,271 + -3.3 102,272 - +1.1 102,272 - -0.8 102,272 - -1.0 102,574 - -0.2 102,574 - -3.7 102,772 + RR42_RS00380 0.32 +0.5 102,773 - RR42_RS00380 0.32 -0.1 102,958 + RR42_RS00380 0.57 +0.5 102,958 + RR42_RS00380 0.57 +1.8 102,959 - RR42_RS00380 0.57 -1.8 102,959 - RR42_RS00380 0.57 -1.0 102,959 - RR42_RS00380 0.57 +0.1 103,030 + RR42_RS00380 0.67 -0.5 103,030 + RR42_RS00380 0.67 -0.0 103,030 + RR42_RS00380 0.67 -1.3 103,030 + RR42_RS00380 0.67 -0.4 103,030 + RR42_RS00380 0.67 -1.1 103,030 + RR42_RS00380 0.67 +1.4 103,031 - RR42_RS00380 0.67 -1.1 103,031 - RR42_RS00380 0.67 -1.0 103,031 - RR42_RS00380 0.67 -0.5 103,084 - RR42_RS00380 0.74 +0.0 103,282 + +1.0 103,282 + +0.4 103,282 + +0.4 103,283 - -1.6 103,283 - -0.1 103,283 - +0.9 103,285 + -0.3 103,285 + -1.8 103,286 - +2.9 103,286 - +0.5 103,286 - -2.2 103,286 - +0.3 103,295 + -0.0 103,510 - RR42_RS00385 0.28 -0.2 103,510 - RR42_RS00385 0.28 -0.6 103,947 + RR42_RS00385 0.80 +0.4 104,109 + +0.2 104,115 + -0.1 104,115 + +0.0 104,116 - +0.0 104,199 + +0.1 104,199 + +1.7 104,203 + +0.7 104,251 + -0.6
Or see this region's nucleotide sequence