Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00295 and RR42_RS00300 are separated by 64 nucleotides RR42_RS00300 and RR42_RS00305 are separated by 87 nucleotides
RR42_RS00295: RR42_RS00295 - DEAD/DEAH box helicase, at 84,587 to 86,008
_RS00295
RR42_RS00300: RR42_RS00300 - kinase, at 86,073 to 86,921
_RS00300
RR42_RS00305: RR42_RS00305 - hypothetical protein, at 87,009 to 88,082
_RS00305
Position (kb) 86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 85.130 kb on + strand, within RR42_RS00295 at 85.130 kb on + strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.651 kb on + strand, within RR42_RS00295 at 85.691 kb on - strand, within RR42_RS00295 at 85.859 kb on + strand, within RR42_RS00295 at 85.907 kb on + strand at 85.907 kb on + strand at 85.907 kb on + strand at 85.908 kb on - strand at 86.166 kb on + strand, within RR42_RS00300 at 86.166 kb on + strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.746 kb on - strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300 at 87.022 kb on + strand at 87.023 kb on - strand at 87.023 kb on - strand at 87.023 kb on - strand at 87.023 kb on - strand at 87.143 kb on + strand, within RR42_RS00305 at 87.144 kb on - strand, within RR42_RS00305 at 87.232 kb on + strand, within RR42_RS00305 at 87.232 kb on + strand, within RR42_RS00305 at 87.310 kb on + strand, within RR42_RS00305 at 87.311 kb on - strand, within RR42_RS00305 at 87.379 kb on + strand, within RR42_RS00305 at 87.379 kb on + strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.380 kb on - strand, within RR42_RS00305 at 87.422 kb on - strand, within RR42_RS00305 at 87.440 kb on - strand, within RR42_RS00305 at 87.482 kb on + strand, within RR42_RS00305 at 87.482 kb on + strand, within RR42_RS00305 at 87.483 kb on - strand, within RR42_RS00305 at 87.644 kb on - strand, within RR42_RS00305 at 87.703 kb on + strand, within RR42_RS00305 at 87.703 kb on + strand, within RR42_RS00305 at 87.703 kb on + strand, within RR42_RS00305 at 87.704 kb on - strand, within RR42_RS00305 at 87.712 kb on + strand, within RR42_RS00305 at 87.751 kb on + strand, within RR42_RS00305 at 87.752 kb on - strand, within RR42_RS00305 at 87.752 kb on - strand, within RR42_RS00305 at 87.752 kb on - strand, within RR42_RS00305 at 87.791 kb on + strand, within RR42_RS00305 at 87.792 kb on - strand, within RR42_RS00305 at 87.811 kb on + strand, within RR42_RS00305 at 87.818 kb on - strand, within RR42_RS00305 at 87.818 kb on - strand, within RR42_RS00305 at 87.888 kb on - strand, within RR42_RS00305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 85,130 + RR42_RS00295 0.38 -1.1 85,130 + RR42_RS00295 0.38 -0.7 85,131 - RR42_RS00295 0.38 -1.6 85,131 - RR42_RS00295 0.38 -1.5 85,131 - RR42_RS00295 0.38 -0.6 85,598 + RR42_RS00295 0.71 -1.9 85,598 + RR42_RS00295 0.71 -0.6 85,598 + RR42_RS00295 0.71 -2.2 85,651 + RR42_RS00295 0.75 +0.4 85,691 - RR42_RS00295 0.78 -1.3 85,859 + RR42_RS00295 0.89 -0.2 85,907 + +0.1 85,907 + -1.8 85,907 + -3.0 85,908 - -0.6 86,166 + RR42_RS00300 0.11 -1.5 86,166 + RR42_RS00300 0.11 -0.8 86,167 - RR42_RS00300 0.11 -0.8 86,167 - RR42_RS00300 0.11 -0.5 86,167 - RR42_RS00300 0.11 -0.6 86,746 - RR42_RS00300 0.79 -2.1 86,769 + RR42_RS00300 0.82 -0.7 86,769 + RR42_RS00300 0.82 -1.2 87,022 + +0.2 87,023 - +0.3 87,023 - -1.5 87,023 - +0.1 87,023 - +2.9 87,143 + RR42_RS00305 0.12 -1.2 87,144 - RR42_RS00305 0.13 +1.8 87,232 + RR42_RS00305 0.21 -1.1 87,232 + RR42_RS00305 0.21 +0.9 87,310 + RR42_RS00305 0.28 -0.2 87,311 - RR42_RS00305 0.28 +0.5 87,379 + RR42_RS00305 0.34 +0.4 87,379 + RR42_RS00305 0.34 +1.6 87,380 - RR42_RS00305 0.35 +0.6 87,380 - RR42_RS00305 0.35 -1.5 87,380 - RR42_RS00305 0.35 -2.2 87,380 - RR42_RS00305 0.35 -1.5 87,380 - RR42_RS00305 0.35 -0.3 87,422 - RR42_RS00305 0.38 -1.6 87,440 - RR42_RS00305 0.40 +0.5 87,482 + RR42_RS00305 0.44 -1.5 87,482 + RR42_RS00305 0.44 -0.1 87,483 - RR42_RS00305 0.44 -2.8 87,644 - RR42_RS00305 0.59 +2.4 87,703 + RR42_RS00305 0.65 +0.6 87,703 + RR42_RS00305 0.65 -0.1 87,703 + RR42_RS00305 0.65 -1.0 87,704 - RR42_RS00305 0.65 +1.4 87,712 + RR42_RS00305 0.65 +0.9 87,751 + RR42_RS00305 0.69 +0.2 87,752 - RR42_RS00305 0.69 -0.6 87,752 - RR42_RS00305 0.69 -1.2 87,752 - RR42_RS00305 0.69 -0.1 87,791 + RR42_RS00305 0.73 -0.2 87,792 - RR42_RS00305 0.73 +0.9 87,811 + RR42_RS00305 0.75 -0.3 87,818 - RR42_RS00305 0.75 +1.2 87,818 - RR42_RS00305 0.75 -0.2 87,888 - RR42_RS00305 0.82 -0.9
Or see this region's nucleotide sequence