Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00105 and RR42_RS00110 are separated by 237 nucleotides RR42_RS00110 and RR42_RS00115 are separated by 155 nucleotides RR42_RS00115 and RR42_RS00120 are separated by 4 nucleotides
RR42_RS00105: RR42_RS00105 - hypothetical protein, at 32,862 to 33,203
_RS00105
RR42_RS00110: RR42_RS00110 - hypothetical protein, at 33,441 to 33,656
_RS00110
RR42_RS00115: RR42_RS00115 - hypothetical protein, at 33,812 to 34,903
_RS00115
RR42_RS00120: RR42_RS00120 - hypothetical protein, at 34,908 to 35,831
_RS00120
Position (kb) 33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 32.959 kb on + strand, within RR42_RS00105 at 32.960 kb on - strand, within RR42_RS00105 at 32.960 kb on - strand, within RR42_RS00105 at 32.960 kb on - strand, within RR42_RS00105 at 33.163 kb on + strand, within RR42_RS00105 at 33.163 kb on + strand, within RR42_RS00105 at 33.163 kb on + strand, within RR42_RS00105 at 33.163 kb on + strand, within RR42_RS00105 at 33.164 kb on - strand, within RR42_RS00105 at 33.230 kb on + strand at 33.231 kb on - strand at 33.231 kb on - strand at 33.231 kb on - strand at 33.298 kb on + strand at 33.299 kb on - strand at 33.354 kb on - strand at 33.773 kb on + strand at 33.778 kb on + strand at 34.009 kb on - strand, within RR42_RS00115 at 34.056 kb on - strand, within RR42_RS00115 at 34.692 kb on - strand, within RR42_RS00115 at 34.942 kb on - strand at 34.942 kb on - strand at 35.104 kb on + strand, within RR42_RS00120 at 35.104 kb on + strand, within RR42_RS00120 at 35.104 kb on + strand, within RR42_RS00120 at 35.148 kb on + strand, within RR42_RS00120 at 35.149 kb on - strand, within RR42_RS00120 at 35.149 kb on - strand, within RR42_RS00120 at 35.194 kb on - strand, within RR42_RS00120 at 35.194 kb on - strand, within RR42_RS00120 at 35.194 kb on - strand, within RR42_RS00120 at 35.219 kb on + strand, within RR42_RS00120 at 35.256 kb on + strand, within RR42_RS00120 at 35.256 kb on + strand, within RR42_RS00120 at 35.257 kb on - strand, within RR42_RS00120 at 35.257 kb on - strand, within RR42_RS00120 at 35.282 kb on + strand, within RR42_RS00120 at 35.282 kb on + strand, within RR42_RS00120 at 35.316 kb on - strand, within RR42_RS00120 at 35.316 kb on - strand, within RR42_RS00120 at 35.316 kb on - strand, within RR42_RS00120 at 35.358 kb on - strand, within RR42_RS00120 at 35.712 kb on + strand, within RR42_RS00120 at 35.712 kb on + strand, within RR42_RS00120 at 35.713 kb on - strand, within RR42_RS00120 at 35.765 kb on - strand at 35.795 kb on + strand at 35.795 kb on + strand at 35.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 32,959 + RR42_RS00105 0.28 -0.4 32,960 - RR42_RS00105 0.29 +0.8 32,960 - RR42_RS00105 0.29 -2.7 32,960 - RR42_RS00105 0.29 +0.4 33,163 + RR42_RS00105 0.88 -1.1 33,163 + RR42_RS00105 0.88 -0.1 33,163 + RR42_RS00105 0.88 -0.2 33,163 + RR42_RS00105 0.88 -1.1 33,164 - RR42_RS00105 0.88 -3.2 33,230 + +1.8 33,231 - -0.2 33,231 - -1.4 33,231 - -0.6 33,298 + -1.1 33,299 - +0.2 33,354 - +0.0 33,773 + +0.9 33,778 + -2.8 34,009 - RR42_RS00115 0.18 -1.5 34,056 - RR42_RS00115 0.22 -1.5 34,692 - RR42_RS00115 0.81 -1.0 34,942 - +0.5 34,942 - -0.2 35,104 + RR42_RS00120 0.21 +0.1 35,104 + RR42_RS00120 0.21 +0.9 35,104 + RR42_RS00120 0.21 -2.6 35,148 + RR42_RS00120 0.26 -0.3 35,149 - RR42_RS00120 0.26 -0.3 35,149 - RR42_RS00120 0.26 -1.1 35,194 - RR42_RS00120 0.31 -0.8 35,194 - RR42_RS00120 0.31 -1.0 35,194 - RR42_RS00120 0.31 +0.2 35,219 + RR42_RS00120 0.34 -0.6 35,256 + RR42_RS00120 0.38 -1.2 35,256 + RR42_RS00120 0.38 -0.6 35,257 - RR42_RS00120 0.38 +1.0 35,257 - RR42_RS00120 0.38 +1.2 35,282 + RR42_RS00120 0.40 +0.0 35,282 + RR42_RS00120 0.40 +0.4 35,316 - RR42_RS00120 0.44 -1.6 35,316 - RR42_RS00120 0.44 -1.0 35,316 - RR42_RS00120 0.44 -0.3 35,358 - RR42_RS00120 0.49 +0.2 35,712 + RR42_RS00120 0.87 -0.5 35,712 + RR42_RS00120 0.87 -0.5 35,713 - RR42_RS00120 0.87 -0.6 35,765 - -0.3 35,795 + +1.5 35,795 + +1.1 35,892 + +0.3
Or see this region's nucleotide sequence