Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00020 and RR42_RS00025 are separated by 225 nucleotides RR42_RS00025 and RR42_RS00030 are separated by 3 nucleotides
RR42_RS00020: RR42_RS00020 - hypothetical protein, at 7,126 to 7,770
_RS00020
RR42_RS00025: RR42_RS00025 - GTPase, at 7,996 to 10,740
_RS00025
RR42_RS00030: RR42_RS00030 - hypothetical protein, at 10,744 to 13,032
_RS00030
Position (kb)
7
8
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 7.032 kb on + strand at 7.033 kb on - strand at 7.037 kb on + strand at 7.037 kb on + strand at 7.093 kb on - strand at 7.098 kb on + strand at 7.099 kb on - strand at 7.099 kb on - strand at 7.151 kb on + strand at 7.304 kb on + strand, within RR42_RS00020 at 7.305 kb on - strand, within RR42_RS00020 at 7.326 kb on + strand, within RR42_RS00020 at 7.329 kb on + strand, within RR42_RS00020 at 7.352 kb on + strand, within RR42_RS00020 at 7.402 kb on - strand, within RR42_RS00020 at 7.607 kb on + strand, within RR42_RS00020 at 7.608 kb on - strand, within RR42_RS00020 at 7.608 kb on - strand, within RR42_RS00020 at 7.642 kb on - strand, within RR42_RS00020 at 7.663 kb on + strand, within RR42_RS00020 at 7.768 kb on + strand at 7.784 kb on + strand at 7.785 kb on - strand at 7.853 kb on - strand at 7.879 kb on - strand at 7.939 kb on + strand at 7.956 kb on + strand at 7.971 kb on + strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.973 kb on + strand at 7.975 kb on + strand at 7.976 kb on - strand at 7.976 kb on - strand at 8.140 kb on + strand at 8.189 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.312 kb on + strand, within RR42_RS00025 at 8.465 kb on + strand, within RR42_RS00025 at 8.465 kb on + strand, within RR42_RS00025 at 8.466 kb on - strand, within RR42_RS00025 at 8.466 kb on - strand, within RR42_RS00025 at 8.755 kb on + strand, within RR42_RS00025 at 8.755 kb on + strand, within RR42_RS00025 at 8.758 kb on + strand, within RR42_RS00025 at 8.758 kb on + strand, within RR42_RS00025 at 8.758 kb on + strand, within RR42_RS00025 at 8.897 kb on - strand, within RR42_RS00025 at 8.906 kb on + strand, within RR42_RS00025 at 8.906 kb on + strand, within RR42_RS00025 at 8.906 kb on + strand, within RR42_RS00025 at 8.907 kb on - strand, within RR42_RS00025 at 8.955 kb on - strand, within RR42_RS00025 at 9.104 kb on + strand, within RR42_RS00025 at 9.104 kb on + strand, within RR42_RS00025 at 9.105 kb on - strand, within RR42_RS00025 at 9.123 kb on + strand, within RR42_RS00025 at 9.158 kb on - strand, within RR42_RS00025 at 9.158 kb on - strand, within RR42_RS00025 at 9.173 kb on + strand, within RR42_RS00025 at 9.295 kb on + strand, within RR42_RS00025 at 9.297 kb on - strand, within RR42_RS00025 at 9.308 kb on - strand, within RR42_RS00025 at 9.354 kb on - strand, within RR42_RS00025 at 9.416 kb on - strand, within RR42_RS00025 at 9.442 kb on + strand, within RR42_RS00025 at 9.442 kb on + strand, within RR42_RS00025 at 9.442 kb on + strand, within RR42_RS00025 at 9.443 kb on - strand, within RR42_RS00025 at 9.443 kb on - strand, within RR42_RS00025 at 9.461 kb on + strand, within RR42_RS00025 at 9.508 kb on + strand, within RR42_RS00025 at 9.508 kb on + strand, within RR42_RS00025 at 9.508 kb on + strand, within RR42_RS00025 at 9.509 kb on - strand, within RR42_RS00025 at 9.509 kb on - strand, within RR42_RS00025 at 9.509 kb on - strand, within RR42_RS00025 at 9.519 kb on - strand, within RR42_RS00025 at 9.541 kb on - strand, within RR42_RS00025 at 9.554 kb on + strand, within RR42_RS00025 at 9.591 kb on - strand, within RR42_RS00025 at 9.602 kb on + strand, within RR42_RS00025 at 9.699 kb on + strand, within RR42_RS00025 at 9.700 kb on - strand, within RR42_RS00025 at 9.700 kb on - strand, within RR42_RS00025 at 9.717 kb on - strand, within RR42_RS00025 at 9.720 kb on + strand, within RR42_RS00025 at 9.720 kb on + strand, within RR42_RS00025 at 9.720 kb on + strand, within RR42_RS00025 at 9.735 kb on - strand, within RR42_RS00025 at 9.783 kb on - strand, within RR42_RS00025 at 9.863 kb on + strand, within RR42_RS00025 at 9.863 kb on + strand, within RR42_RS00025 at 9.953 kb on - strand, within RR42_RS00025 at 9.953 kb on - strand, within RR42_RS00025 at 9.990 kb on - strand, within RR42_RS00025 at 10.010 kb on + strand, within RR42_RS00025 at 10.010 kb on + strand, within RR42_RS00025 at 10.011 kb on - strand, within RR42_RS00025 at 10.086 kb on + strand, within RR42_RS00025 at 10.130 kb on + strand, within RR42_RS00025 at 10.159 kb on - strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.163 kb on - strand, within RR42_RS00025 at 10.163 kb on - strand, within RR42_RS00025 at 10.163 kb on - strand, within RR42_RS00025 at 10.251 kb on + strand, within RR42_RS00025 at 10.357 kb on - strand, within RR42_RS00025 at 10.388 kb on + strand, within RR42_RS00025 at 10.388 kb on + strand, within RR42_RS00025 at 10.389 kb on - strand, within RR42_RS00025 at 10.396 kb on + strand, within RR42_RS00025 at 10.397 kb on - strand, within RR42_RS00025 at 10.397 kb on - strand, within RR42_RS00025 at 10.398 kb on + strand, within RR42_RS00025 at 10.399 kb on - strand, within RR42_RS00025 at 10.399 kb on - strand, within RR42_RS00025 at 10.468 kb on - strand at 10.504 kb on - strand at 10.615 kb on - strand at 10.615 kb on - strand at 10.615 kb on - strand at 10.615 kb on - strand at 10.643 kb on + strand at 10.644 kb on - strand at 10.664 kb on + strand at 10.664 kb on + strand at 10.706 kb on + strand at 10.707 kb on - strand at 10.707 kb on - strand at 10.750 kb on - strand at 10.805 kb on + strand at 10.814 kb on + strand at 10.814 kb on + strand at 10.836 kb on + strand at 10.836 kb on + strand at 10.965 kb on + strand at 10.965 kb on + strand at 10.965 kb on + strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 11.042 kb on + strand, within RR42_RS00030 at 11.112 kb on - strand, within RR42_RS00030 at 11.131 kb on + strand, within RR42_RS00030 at 11.132 kb on - strand, within RR42_RS00030 at 11.132 kb on - strand, within RR42_RS00030 at 11.143 kb on + strand, within RR42_RS00030 at 11.236 kb on - strand, within RR42_RS00030 at 11.236 kb on - strand, within RR42_RS00030 at 11.313 kb on + strand, within RR42_RS00030 at 11.365 kb on - strand, within RR42_RS00030 at 11.388 kb on - strand, within RR42_RS00030 at 11.613 kb on + strand, within RR42_RS00030 at 11.613 kb on + strand, within RR42_RS00030 at 11.614 kb on - strand, within RR42_RS00030 at 11.694 kb on + strand, within RR42_RS00030 at 11.695 kb on - strand, within RR42_RS00030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 7,032 + -1.6 7,033 - +1.0 7,037 + +0.0 7,037 + -0.6 7,093 - +1.1 7,098 + +0.2 7,099 - +0.9 7,099 - -0.6 7,151 + +0.4 7,304 + RR42_RS00020 0.28 -0.3 7,305 - RR42_RS00020 0.28 +0.1 7,326 + RR42_RS00020 0.31 +0.9 7,329 + RR42_RS00020 0.31 +2.0 7,352 + RR42_RS00020 0.35 +1.6 7,402 - RR42_RS00020 0.43 +1.4 7,607 + RR42_RS00020 0.75 -1.5 7,608 - RR42_RS00020 0.75 -2.0 7,608 - RR42_RS00020 0.75 +0.9 7,642 - RR42_RS00020 0.80 +0.0 7,663 + RR42_RS00020 0.83 +0.4 7,768 + -0.8 7,784 + -0.6 7,785 - -1.6 7,853 - -2.2 7,879 - -1.2 7,939 + -0.4 7,956 + -0.5 7,971 + +0.9 7,972 - -1.6 7,972 - +1.3 7,973 + +2.0 7,975 + +1.4 7,976 - +0.4 7,976 - -0.7 8,140 + +1.0 8,189 + +0.8 8,253 + +1.5 8,253 + -0.9 8,253 + +0.5 8,253 + -1.5 8,253 + +2.0 8,253 + -1.8 8,257 + -1.3 8,257 + -1.1 8,257 + +1.2 8,257 + -0.3 8,257 + +0.7 8,257 + -0.3 8,258 - -0.1 8,258 - +0.9 8,258 - +0.4 8,258 - +0.9 8,258 - +0.1 8,312 + RR42_RS00025 0.12 -0.9 8,465 + RR42_RS00025 0.17 +0.7 8,465 + RR42_RS00025 0.17 -2.3 8,466 - RR42_RS00025 0.17 -0.2 8,466 - RR42_RS00025 0.17 +0.6 8,755 + RR42_RS00025 0.28 -1.6 8,755 + RR42_RS00025 0.28 -0.5 8,758 + RR42_RS00025 0.28 -0.1 8,758 + RR42_RS00025 0.28 +1.4 8,758 + RR42_RS00025 0.28 +0.0 8,897 - RR42_RS00025 0.33 +0.2 8,906 + RR42_RS00025 0.33 +2.0 8,906 + RR42_RS00025 0.33 -1.6 8,906 + RR42_RS00025 0.33 -0.6 8,907 - RR42_RS00025 0.33 -0.0 8,955 - RR42_RS00025 0.35 +0.4 9,104 + RR42_RS00025 0.40 -1.2 9,104 + RR42_RS00025 0.40 -1.5 9,105 - RR42_RS00025 0.40 -1.8 9,123 + RR42_RS00025 0.41 +1.2 9,158 - RR42_RS00025 0.42 +0.7 9,158 - RR42_RS00025 0.42 -0.2 9,173 + RR42_RS00025 0.43 +0.2 9,295 + RR42_RS00025 0.47 +0.6 9,297 - RR42_RS00025 0.47 +0.9 9,308 - RR42_RS00025 0.48 +1.4 9,354 - RR42_RS00025 0.49 +0.8 9,416 - RR42_RS00025 0.52 +1.2 9,442 + RR42_RS00025 0.53 +4.4 9,442 + RR42_RS00025 0.53 +0.6 9,442 + RR42_RS00025 0.53 +0.6 9,443 - RR42_RS00025 0.53 +2.2 9,443 - RR42_RS00025 0.53 +0.4 9,461 + RR42_RS00025 0.53 +0.4 9,508 + RR42_RS00025 0.55 +0.5 9,508 + RR42_RS00025 0.55 +1.4 9,508 + RR42_RS00025 0.55 +0.2 9,509 - RR42_RS00025 0.55 -0.1 9,509 - RR42_RS00025 0.55 +1.6 9,509 - RR42_RS00025 0.55 -0.1 9,519 - RR42_RS00025 0.55 +0.7 9,541 - RR42_RS00025 0.56 -0.6 9,554 + RR42_RS00025 0.57 +1.5 9,591 - RR42_RS00025 0.58 +1.0 9,602 + RR42_RS00025 0.59 -0.3 9,699 + RR42_RS00025 0.62 +0.4 9,700 - RR42_RS00025 0.62 -0.5 9,700 - RR42_RS00025 0.62 -1.2 9,717 - RR42_RS00025 0.63 +1.0 9,720 + RR42_RS00025 0.63 -0.5 9,720 + RR42_RS00025 0.63 -0.6 9,720 + RR42_RS00025 0.63 +0.0 9,735 - RR42_RS00025 0.63 +0.3 9,783 - RR42_RS00025 0.65 -1.4 9,863 + RR42_RS00025 0.68 +0.1 9,863 + RR42_RS00025 0.68 +0.8 9,953 - RR42_RS00025 0.71 -0.6 9,953 - RR42_RS00025 0.71 -1.5 9,990 - RR42_RS00025 0.73 +0.8 10,010 + RR42_RS00025 0.73 +0.1 10,010 + RR42_RS00025 0.73 +0.8 10,011 - RR42_RS00025 0.73 -0.6 10,086 + RR42_RS00025 0.76 +0.8 10,130 + RR42_RS00025 0.78 -0.9 10,159 - RR42_RS00025 0.79 -0.0 10,162 + RR42_RS00025 0.79 +0.8 10,162 + RR42_RS00025 0.79 -0.1 10,162 + RR42_RS00025 0.79 -2.3 10,162 + RR42_RS00025 0.79 +1.8 10,162 + RR42_RS00025 0.79 -1.3 10,163 - RR42_RS00025 0.79 +0.4 10,163 - RR42_RS00025 0.79 -1.8 10,163 - RR42_RS00025 0.79 +1.8 10,251 + RR42_RS00025 0.82 +1.3 10,357 - RR42_RS00025 0.86 -3.2 10,388 + RR42_RS00025 0.87 +2.4 10,388 + RR42_RS00025 0.87 -0.4 10,389 - RR42_RS00025 0.87 -0.1 10,396 + RR42_RS00025 0.87 +1.4 10,397 - RR42_RS00025 0.87 -2.5 10,397 - RR42_RS00025 0.87 -0.1 10,398 + RR42_RS00025 0.88 -1.0 10,399 - RR42_RS00025 0.88 -0.5 10,399 - RR42_RS00025 0.88 +1.1 10,468 - -0.6 10,504 - -2.3 10,615 - -1.5 10,615 - +1.0 10,615 - -0.6 10,615 - +1.9 10,643 + +0.0 10,644 - +0.3 10,664 + +1.7 10,664 + +0.2 10,706 + -0.0 10,707 - -2.9 10,707 - +0.1 10,750 - -1.0 10,805 + +0.9 10,814 + +1.3 10,814 + -0.2 10,836 + +0.5 10,836 + +0.6 10,965 + +0.5 10,965 + +0.4 10,965 + +1.0 10,966 - +0.4 10,966 - +0.1 10,966 - -1.2 10,966 - +1.2 10,966 - +0.4 10,966 - -1.3 11,042 + RR42_RS00030 0.13 +1.5 11,112 - RR42_RS00030 0.16 -0.4 11,131 + RR42_RS00030 0.17 +0.6 11,132 - RR42_RS00030 0.17 +1.4 11,132 - RR42_RS00030 0.17 -0.2 11,143 + RR42_RS00030 0.17 +0.2 11,236 - RR42_RS00030 0.21 +1.8 11,236 - RR42_RS00030 0.21 -2.0 11,313 + RR42_RS00030 0.25 -1.0 11,365 - RR42_RS00030 0.27 +1.4 11,388 - RR42_RS00030 0.28 -2.2 11,613 + RR42_RS00030 0.38 -0.2 11,613 + RR42_RS00030 0.38 -0.1 11,614 - RR42_RS00030 0.38 -0.9 11,694 + RR42_RS00030 0.42 +0.7 11,695 - RR42_RS00030 0.42 -1.6
Or see this region's nucleotide sequence