Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_959 and Pf6N2E2_960 are separated by 82 nucleotides Pf6N2E2_960 and Pf6N2E2_961 overlap by 4 nucleotides Pf6N2E2_961 and Pf6N2E2_962 overlap by 4 nucleotides
Pf6N2E2_959 - putative membrane protein, at 1,130,027 to 1,130,761
_959
Pf6N2E2_960 - Type IV secretory pathway, VirD4 components, at 1,130,844 to 1,132,964
_960
Pf6N2E2_961 - FIG00954712: hypothetical protein, at 1,132,961 to 1,133,476
_961
Pf6N2E2_962 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 1,133,473 to 1,133,988
_962
Position (kb)
1130
1131
1132
1133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1130.031 kb on - strand at 1130.031 kb on - strand at 1130.137 kb on - strand, within Pf6N2E2_959 at 1130.337 kb on + strand, within Pf6N2E2_959 at 1130.530 kb on - strand, within Pf6N2E2_959 at 1130.582 kb on - strand, within Pf6N2E2_959 at 1130.582 kb on - strand, within Pf6N2E2_959 at 1130.615 kb on + strand, within Pf6N2E2_959 at 1130.615 kb on + strand, within Pf6N2E2_959 at 1130.811 kb on + strand at 1130.873 kb on + strand at 1130.873 kb on + strand at 1131.275 kb on + strand, within Pf6N2E2_960 at 1131.309 kb on + strand, within Pf6N2E2_960 at 1131.309 kb on + strand, within Pf6N2E2_960 at 1131.343 kb on + strand, within Pf6N2E2_960 at 1131.344 kb on - strand, within Pf6N2E2_960 at 1131.503 kb on - strand, within Pf6N2E2_960 at 1131.503 kb on - strand, within Pf6N2E2_960 at 1131.596 kb on - strand, within Pf6N2E2_960 at 1131.665 kb on - strand, within Pf6N2E2_960 at 1131.678 kb on + strand, within Pf6N2E2_960 at 1131.679 kb on - strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.752 kb on + strand, within Pf6N2E2_960 at 1131.752 kb on + strand, within Pf6N2E2_960 at 1131.802 kb on - strand, within Pf6N2E2_960 at 1131.860 kb on + strand, within Pf6N2E2_960 at 1131.956 kb on - strand, within Pf6N2E2_960 at 1132.049 kb on + strand, within Pf6N2E2_960 at 1132.050 kb on - strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.119 kb on - strand, within Pf6N2E2_960 at 1132.165 kb on + strand, within Pf6N2E2_960 at 1132.165 kb on + strand, within Pf6N2E2_960 at 1132.191 kb on + strand, within Pf6N2E2_960 at 1132.192 kb on - strand, within Pf6N2E2_960 at 1132.204 kb on + strand, within Pf6N2E2_960 at 1132.243 kb on + strand, within Pf6N2E2_960 at 1132.243 kb on + strand, within Pf6N2E2_960 at 1132.243 kb on + strand, within Pf6N2E2_960 at 1132.244 kb on - strand, within Pf6N2E2_960 at 1132.244 kb on - strand, within Pf6N2E2_960 at 1132.330 kb on + strand, within Pf6N2E2_960 at 1132.330 kb on + strand, within Pf6N2E2_960 at 1132.412 kb on + strand, within Pf6N2E2_960 at 1132.412 kb on + strand, within Pf6N2E2_960 at 1132.413 kb on - strand, within Pf6N2E2_960 at 1132.461 kb on + strand, within Pf6N2E2_960 at 1132.577 kb on + strand, within Pf6N2E2_960 at 1132.577 kb on + strand, within Pf6N2E2_960 at 1132.578 kb on - strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.600 kb on + strand, within Pf6N2E2_960 at 1132.601 kb on - strand, within Pf6N2E2_960 at 1132.804 kb on + strand at 1132.862 kb on - strand at 1132.862 kb on - strand at 1132.897 kb on - strand at 1132.903 kb on + strand at 1132.903 kb on + strand at 1132.903 kb on + strand at 1132.903 kb on + strand at 1132.904 kb on - strand at 1133.189 kb on + strand, within Pf6N2E2_961 at 1133.207 kb on + strand, within Pf6N2E2_961 at 1133.345 kb on + strand, within Pf6N2E2_961 at 1133.458 kb on + strand at 1133.459 kb on - strand at 1133.465 kb on + strand at 1133.812 kb on + strand, within Pf6N2E2_962 at 1133.812 kb on + strand, within Pf6N2E2_962 at 1133.932 kb on + strand, within Pf6N2E2_962
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 1,130,031 - -0.2 1,130,031 - +0.7 1,130,137 - Pf6N2E2_959 0.15 +0.8 1,130,337 + Pf6N2E2_959 0.42 +0.4 1,130,530 - Pf6N2E2_959 0.68 +2.5 1,130,582 - Pf6N2E2_959 0.76 -1.9 1,130,582 - Pf6N2E2_959 0.76 -0.2 1,130,615 + Pf6N2E2_959 0.80 +0.1 1,130,615 + Pf6N2E2_959 0.80 +0.2 1,130,811 + -0.9 1,130,873 + +1.1 1,130,873 + +1.2 1,131,275 + Pf6N2E2_960 0.20 -0.5 1,131,309 + Pf6N2E2_960 0.22 +0.1 1,131,309 + Pf6N2E2_960 0.22 -0.3 1,131,343 + Pf6N2E2_960 0.24 -0.1 1,131,344 - Pf6N2E2_960 0.24 -1.7 1,131,503 - Pf6N2E2_960 0.31 +0.5 1,131,503 - Pf6N2E2_960 0.31 +0.2 1,131,596 - Pf6N2E2_960 0.35 -0.1 1,131,665 - Pf6N2E2_960 0.39 -3.1 1,131,678 + Pf6N2E2_960 0.39 -0.1 1,131,679 - Pf6N2E2_960 0.39 +1.4 1,131,748 + Pf6N2E2_960 0.43 -1.0 1,131,748 + Pf6N2E2_960 0.43 -0.3 1,131,748 + Pf6N2E2_960 0.43 -0.1 1,131,752 + Pf6N2E2_960 0.43 -0.6 1,131,752 + Pf6N2E2_960 0.43 -0.1 1,131,802 - Pf6N2E2_960 0.45 -0.6 1,131,860 + Pf6N2E2_960 0.48 -0.4 1,131,956 - Pf6N2E2_960 0.52 -0.1 1,132,049 + Pf6N2E2_960 0.57 +0.4 1,132,050 - Pf6N2E2_960 0.57 -1.9 1,132,118 + Pf6N2E2_960 0.60 -0.0 1,132,118 + Pf6N2E2_960 0.60 -1.9 1,132,118 + Pf6N2E2_960 0.60 -0.7 1,132,118 + Pf6N2E2_960 0.60 -0.4 1,132,119 - Pf6N2E2_960 0.60 -1.0 1,132,165 + Pf6N2E2_960 0.62 +0.8 1,132,165 + Pf6N2E2_960 0.62 -0.8 1,132,191 + Pf6N2E2_960 0.64 +0.1 1,132,192 - Pf6N2E2_960 0.64 +0.4 1,132,204 + Pf6N2E2_960 0.64 +0.7 1,132,243 + Pf6N2E2_960 0.66 +0.7 1,132,243 + Pf6N2E2_960 0.66 -2.9 1,132,243 + Pf6N2E2_960 0.66 +0.3 1,132,244 - Pf6N2E2_960 0.66 +0.9 1,132,244 - Pf6N2E2_960 0.66 -0.6 1,132,330 + Pf6N2E2_960 0.70 +0.4 1,132,330 + Pf6N2E2_960 0.70 +0.0 1,132,412 + Pf6N2E2_960 0.74 -2.0 1,132,412 + Pf6N2E2_960 0.74 +0.8 1,132,413 - Pf6N2E2_960 0.74 -0.5 1,132,461 + Pf6N2E2_960 0.76 +0.3 1,132,577 + Pf6N2E2_960 0.82 +0.5 1,132,577 + Pf6N2E2_960 0.82 -0.4 1,132,578 - Pf6N2E2_960 0.82 +0.2 1,132,579 + Pf6N2E2_960 0.82 +0.6 1,132,579 + Pf6N2E2_960 0.82 +1.9 1,132,579 + Pf6N2E2_960 0.82 -0.1 1,132,579 + Pf6N2E2_960 0.82 -0.4 1,132,579 + Pf6N2E2_960 0.82 +0.2 1,132,579 + Pf6N2E2_960 0.82 -1.8 1,132,600 + Pf6N2E2_960 0.83 -0.7 1,132,601 - Pf6N2E2_960 0.83 +0.1 1,132,804 + +0.8 1,132,862 - +0.4 1,132,862 - -0.3 1,132,897 - +0.2 1,132,903 + -0.6 1,132,903 + +1.2 1,132,903 + +0.3 1,132,903 + +0.7 1,132,904 - -0.6 1,133,189 + Pf6N2E2_961 0.44 +0.3 1,133,207 + Pf6N2E2_961 0.48 -0.3 1,133,345 + Pf6N2E2_961 0.74 -0.3 1,133,458 + +0.1 1,133,459 - -2.2 1,133,465 + +0.4 1,133,812 + Pf6N2E2_962 0.66 +1.1 1,133,812 + Pf6N2E2_962 0.66 +0.0 1,133,932 + Pf6N2E2_962 0.89 -1.3
Or see this region's nucleotide sequence