Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4509 and Pf6N2E2_4510 are separated by 383 nucleotides Pf6N2E2_4510 and Pf6N2E2_4511 are separated by 40 nucleotides
Pf6N2E2_4509 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 5,240,232 to 5,241,503
_4509
Pf6N2E2_4510 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) (from data) , at 5,241,887 to 5,242,663
_4510
Pf6N2E2_4511 - glutamine synthetase family protein, at 5,242,704 to 5,244,062
_4511
Position (kb)
5241
5242
5243 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5241.049 kb on + strand, within Pf6N2E2_4509 at 5241.049 kb on + strand, within Pf6N2E2_4509 at 5241.050 kb on - strand, within Pf6N2E2_4509 at 5241.050 kb on - strand, within Pf6N2E2_4509 at 5241.074 kb on - strand, within Pf6N2E2_4509 at 5241.074 kb on - strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.161 kb on - strand, within Pf6N2E2_4509 at 5241.402 kb on - strand at 5241.426 kb on + strand at 5241.426 kb on + strand at 5241.427 kb on - strand at 5241.457 kb on + strand at 5241.483 kb on + strand at 5241.484 kb on - strand at 5241.484 kb on - strand at 5241.512 kb on + strand at 5241.535 kb on + strand at 5241.536 kb on - strand at 5241.536 kb on - strand at 5241.579 kb on - strand at 5241.628 kb on + strand at 5241.634 kb on + strand at 5241.803 kb on - strand at 5241.825 kb on - strand at 5241.842 kb on + strand at 5241.870 kb on + strand at 5241.870 kb on + strand at 5241.870 kb on + strand at 5241.871 kb on - strand at 5241.873 kb on + strand at 5241.947 kb on + strand at 5241.947 kb on + strand at 5241.968 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.099 kb on - strand, within Pf6N2E2_4510 at 5242.248 kb on + strand, within Pf6N2E2_4510 at 5242.248 kb on + strand, within Pf6N2E2_4510 at 5242.249 kb on - strand, within Pf6N2E2_4510 at 5242.249 kb on - strand, within Pf6N2E2_4510 at 5242.319 kb on - strand, within Pf6N2E2_4510 at 5242.319 kb on - strand, within Pf6N2E2_4510 at 5242.340 kb on + strand, within Pf6N2E2_4510 at 5242.364 kb on - strand, within Pf6N2E2_4510 at 5242.380 kb on + strand, within Pf6N2E2_4510 at 5242.380 kb on + strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.411 kb on - strand, within Pf6N2E2_4510 at 5242.411 kb on - strand, within Pf6N2E2_4510 at 5242.533 kb on + strand, within Pf6N2E2_4510 at 5242.534 kb on - strand, within Pf6N2E2_4510 at 5242.534 kb on - strand, within Pf6N2E2_4510 at 5242.534 kb on - strand, within Pf6N2E2_4510 at 5242.563 kb on + strand, within Pf6N2E2_4510 at 5242.563 kb on + strand, within Pf6N2E2_4510 at 5242.563 kb on + strand, within Pf6N2E2_4510 at 5242.564 kb on - strand, within Pf6N2E2_4510 at 5242.571 kb on - strand, within Pf6N2E2_4510 at 5242.580 kb on + strand, within Pf6N2E2_4510 at 5242.580 kb on + strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.668 kb on + strand at 5242.668 kb on + strand at 5242.669 kb on - strand at 5242.669 kb on - strand at 5242.671 kb on + strand at 5242.672 kb on - strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.704 kb on - strand at 5242.704 kb on - strand at 5242.710 kb on - strand at 5242.887 kb on + strand, within Pf6N2E2_4511 at 5242.887 kb on + strand, within Pf6N2E2_4511 at 5242.888 kb on - strand, within Pf6N2E2_4511 at 5242.888 kb on - strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.909 kb on - strand, within Pf6N2E2_4511 at 5242.909 kb on - strand, within Pf6N2E2_4511 at 5242.966 kb on + strand, within Pf6N2E2_4511 at 5243.019 kb on + strand, within Pf6N2E2_4511 at 5243.020 kb on - strand, within Pf6N2E2_4511 at 5243.074 kb on + strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.085 kb on + strand, within Pf6N2E2_4511 at 5243.086 kb on - strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.134 kb on - strand, within Pf6N2E2_4511 at 5243.269 kb on - strand, within Pf6N2E2_4511 at 5243.431 kb on + strand, within Pf6N2E2_4511 at 5243.431 kb on + strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.606 kb on - strand, within Pf6N2E2_4511
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 5,240,911 + Pf6N2E2_4509 0.53 -1.2 5,240,911 + Pf6N2E2_4509 0.53 -1.0 5,240,911 + Pf6N2E2_4509 0.53 -0.5 5,241,049 + Pf6N2E2_4509 0.64 -0.1 5,241,049 + Pf6N2E2_4509 0.64 +0.2 5,241,050 - Pf6N2E2_4509 0.64 +1.1 5,241,050 - Pf6N2E2_4509 0.64 +0.8 5,241,074 - Pf6N2E2_4509 0.66 -0.0 5,241,074 - Pf6N2E2_4509 0.66 -0.4 5,241,160 + Pf6N2E2_4509 0.73 -1.4 5,241,160 + Pf6N2E2_4509 0.73 -0.2 5,241,160 + Pf6N2E2_4509 0.73 +0.8 5,241,161 - Pf6N2E2_4509 0.73 -0.7 5,241,402 - -0.2 5,241,426 + -0.4 5,241,426 + +0.3 5,241,427 - -0.7 5,241,457 + +0.3 5,241,483 + -0.1 5,241,484 - -0.6 5,241,484 - -0.3 5,241,512 + +0.5 5,241,535 + -1.2 5,241,536 - +0.5 5,241,536 - -1.9 5,241,579 - +0.2 5,241,628 + -0.2 5,241,634 + +0.4 5,241,803 - +1.1 5,241,825 - +1.7 5,241,842 + +0.1 5,241,870 + +0.7 5,241,870 + +0.7 5,241,870 + +0.2 5,241,871 - -0.1 5,241,873 + +1.4 5,241,947 + +0.6 5,241,947 + +1.0 5,241,968 + Pf6N2E2_4510 0.10 +1.2 5,242,098 + Pf6N2E2_4510 0.27 +1.3 5,242,098 + Pf6N2E2_4510 0.27 +1.2 5,242,098 + Pf6N2E2_4510 0.27 +1.9 5,242,098 + Pf6N2E2_4510 0.27 +1.2 5,242,098 + Pf6N2E2_4510 0.27 +1.9 5,242,099 - Pf6N2E2_4510 0.27 +0.4 5,242,248 + Pf6N2E2_4510 0.46 +2.2 5,242,248 + Pf6N2E2_4510 0.46 +1.2 5,242,249 - Pf6N2E2_4510 0.47 +2.0 5,242,249 - Pf6N2E2_4510 0.47 +1.7 5,242,319 - Pf6N2E2_4510 0.56 -0.7 5,242,319 - Pf6N2E2_4510 0.56 +0.6 5,242,340 + Pf6N2E2_4510 0.58 +3.0 5,242,364 - Pf6N2E2_4510 0.61 +0.9 5,242,380 + Pf6N2E2_4510 0.63 +4.0 5,242,380 + Pf6N2E2_4510 0.63 +1.4 5,242,381 - Pf6N2E2_4510 0.64 +2.3 5,242,381 - Pf6N2E2_4510 0.64 +0.1 5,242,381 - Pf6N2E2_4510 0.64 +0.1 5,242,381 - Pf6N2E2_4510 0.64 +1.1 5,242,381 - Pf6N2E2_4510 0.64 +1.0 5,242,410 + Pf6N2E2_4510 0.67 +2.0 5,242,410 + Pf6N2E2_4510 0.67 +2.5 5,242,410 + Pf6N2E2_4510 0.67 +2.5 5,242,410 + Pf6N2E2_4510 0.67 +3.8 5,242,411 - Pf6N2E2_4510 0.67 +1.7 5,242,411 - Pf6N2E2_4510 0.67 +0.2 5,242,533 + Pf6N2E2_4510 0.83 +1.7 5,242,534 - Pf6N2E2_4510 0.83 +1.1 5,242,534 - Pf6N2E2_4510 0.83 +0.3 5,242,534 - Pf6N2E2_4510 0.83 +0.5 5,242,563 + Pf6N2E2_4510 0.87 +1.9 5,242,563 + Pf6N2E2_4510 0.87 +2.3 5,242,563 + Pf6N2E2_4510 0.87 +2.1 5,242,564 - Pf6N2E2_4510 0.87 -1.4 5,242,571 - Pf6N2E2_4510 0.88 +0.7 5,242,580 + Pf6N2E2_4510 0.89 +2.2 5,242,580 + Pf6N2E2_4510 0.89 +2.2 5,242,581 - Pf6N2E2_4510 0.89 +1.0 5,242,581 - Pf6N2E2_4510 0.89 +1.4 5,242,581 - Pf6N2E2_4510 0.89 +1.7 5,242,581 - Pf6N2E2_4510 0.89 +1.0 5,242,581 - Pf6N2E2_4510 0.89 +0.3 5,242,668 + +1.6 5,242,668 + +2.3 5,242,669 - +1.2 5,242,669 - +0.2 5,242,671 + +2.4 5,242,672 - +0.8 5,242,703 + +1.5 5,242,703 + +0.6 5,242,703 + -0.5 5,242,703 + +0.5 5,242,703 + +0.4 5,242,703 + -0.4 5,242,704 - +0.4 5,242,704 - +0.7 5,242,710 - +0.3 5,242,887 + Pf6N2E2_4511 0.13 +1.1 5,242,887 + Pf6N2E2_4511 0.13 +1.8 5,242,888 - Pf6N2E2_4511 0.14 +1.6 5,242,888 - Pf6N2E2_4511 0.14 +1.8 5,242,905 + Pf6N2E2_4511 0.15 -0.0 5,242,905 + Pf6N2E2_4511 0.15 +0.4 5,242,905 + Pf6N2E2_4511 0.15 +1.4 5,242,905 + Pf6N2E2_4511 0.15 +1.5 5,242,905 + Pf6N2E2_4511 0.15 +0.8 5,242,905 + Pf6N2E2_4511 0.15 +0.5 5,242,905 + Pf6N2E2_4511 0.15 +1.3 5,242,905 + Pf6N2E2_4511 0.15 +2.5 5,242,905 + Pf6N2E2_4511 0.15 +0.7 5,242,905 + Pf6N2E2_4511 0.15 +0.6 5,242,906 - Pf6N2E2_4511 0.15 +1.2 5,242,906 - Pf6N2E2_4511 0.15 +0.2 5,242,906 - Pf6N2E2_4511 0.15 +1.2 5,242,906 - Pf6N2E2_4511 0.15 +1.8 5,242,906 - Pf6N2E2_4511 0.15 +0.9 5,242,906 - Pf6N2E2_4511 0.15 +1.4 5,242,906 - Pf6N2E2_4511 0.15 +1.6 5,242,906 - Pf6N2E2_4511 0.15 +0.8 5,242,906 - Pf6N2E2_4511 0.15 +1.5 5,242,909 - Pf6N2E2_4511 0.15 +0.9 5,242,909 - Pf6N2E2_4511 0.15 +1.2 5,242,966 + Pf6N2E2_4511 0.19 +0.3 5,243,019 + Pf6N2E2_4511 0.23 +1.1 5,243,020 - Pf6N2E2_4511 0.23 -0.1 5,243,074 + Pf6N2E2_4511 0.27 +1.1 5,243,075 - Pf6N2E2_4511 0.27 +1.3 5,243,075 - Pf6N2E2_4511 0.27 +1.3 5,243,075 - Pf6N2E2_4511 0.27 +1.0 5,243,075 - Pf6N2E2_4511 0.27 +1.4 5,243,085 + Pf6N2E2_4511 0.28 +1.9 5,243,086 - Pf6N2E2_4511 0.28 +0.8 5,243,133 + Pf6N2E2_4511 0.32 +2.6 5,243,133 + Pf6N2E2_4511 0.32 +2.6 5,243,133 + Pf6N2E2_4511 0.32 +2.5 5,243,133 + Pf6N2E2_4511 0.32 +2.2 5,243,133 + Pf6N2E2_4511 0.32 +0.5 5,243,134 - Pf6N2E2_4511 0.32 -0.2 5,243,269 - Pf6N2E2_4511 0.42 +1.4 5,243,431 + Pf6N2E2_4511 0.53 +0.8 5,243,431 + Pf6N2E2_4511 0.53 -0.0 5,243,432 - Pf6N2E2_4511 0.54 +1.3 5,243,432 - Pf6N2E2_4511 0.54 +0.7 5,243,432 - Pf6N2E2_4511 0.54 +1.4 5,243,432 - Pf6N2E2_4511 0.54 +1.2 5,243,432 - Pf6N2E2_4511 0.54 +1.3 5,243,432 - Pf6N2E2_4511 0.54 +0.4 5,243,606 - Pf6N2E2_4511 0.66 +0.7
Or see this region's nucleotide sequence