Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3783 and Pf6N2E2_3784 are separated by 24 nucleotides Pf6N2E2_3784 and Pf6N2E2_3785 are separated by 10 nucleotides Pf6N2E2_3785 and Pf6N2E2_3786 overlap by 4 nucleotides Pf6N2E2_3786 and Pf6N2E2_3787 overlap by 4 nucleotides Pf6N2E2_3787 and Pf6N2E2_3788 are separated by 159 nucleotides
Pf6N2E2_3783 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 4,405,909 to 4,406,241
_3783
Pf6N2E2_3784 - Twin-arginine translocation protein TatA, at 4,406,266 to 4,406,553
_3784
Pf6N2E2_3785 - Twin-arginine translocation protein TatB, at 4,406,564 to 4,406,986
_3785
Pf6N2E2_3786 - Twin-arginine translocation protein TatC, at 4,406,983 to 4,407,780
_3786
Pf6N2E2_3787 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 4,407,777 to 4,408,484
_3787
Pf6N2E2_3788 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 4,408,644 to 4,410,560
_3788
Position (kb)
4406
4407
4408 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.102 kb on - strand, within Pf6N2E2_3783 at 4406.271 kb on + strand at 4406.562 kb on + strand at 4406.562 kb on + strand at 4406.562 kb on + strand at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.765 kb on - strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.962 kb on + strand at 4406.962 kb on + strand at 4406.962 kb on + strand at 4407.292 kb on + strand, within Pf6N2E2_3786 at 4407.370 kb on + strand, within Pf6N2E2_3786 at 4407.370 kb on + strand, within Pf6N2E2_3786 at 4407.373 kb on + strand, within Pf6N2E2_3786 at 4407.454 kb on + strand, within Pf6N2E2_3786 at 4407.454 kb on + strand, within Pf6N2E2_3786 at 4407.556 kb on + strand, within Pf6N2E2_3786 at 4407.577 kb on + strand, within Pf6N2E2_3786 at 4407.577 kb on + strand, within Pf6N2E2_3786 at 4407.577 kb on + strand, within Pf6N2E2_3786 at 4408.491 kb on + strand at 4408.491 kb on + strand at 4408.491 kb on + strand at 4408.491 kb on + strand at 4408.491 kb on + strand at 4408.491 kb on + strand at 4408.492 kb on - strand at 4408.492 kb on - strand at 4408.558 kb on - strand at 4408.576 kb on + strand at 4408.577 kb on - strand at 4408.591 kb on + strand at 4408.591 kb on + strand at 4408.591 kb on + strand at 4408.592 kb on - strand at 4408.592 kb on - strand at 4408.611 kb on + strand at 4408.611 kb on + strand at 4408.618 kb on + strand at 4408.619 kb on - strand at 4408.619 kb on - strand at 4408.619 kb on - strand at 4408.619 kb on - strand at 4408.619 kb on - strand at 4408.620 kb on + strand at 4408.621 kb on - strand at 4408.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 4,406,101 + Pf6N2E2_3783 0.58 -2.7 4,406,101 + Pf6N2E2_3783 0.58 -3.9 4,406,101 + Pf6N2E2_3783 0.58 -3.8 4,406,101 + Pf6N2E2_3783 0.58 -3.6 4,406,101 + Pf6N2E2_3783 0.58 -3.7 4,406,102 - Pf6N2E2_3783 0.58 -2.6 4,406,271 + -1.5 4,406,562 + -2.4 4,406,562 + -0.6 4,406,562 + -2.2 4,406,764 + Pf6N2E2_3785 0.47 -0.8 4,406,764 + Pf6N2E2_3785 0.47 -0.7 4,406,764 + Pf6N2E2_3785 0.47 -0.5 4,406,764 + Pf6N2E2_3785 0.47 -1.9 4,406,764 + Pf6N2E2_3785 0.47 -2.3 4,406,764 + Pf6N2E2_3785 0.47 -3.0 4,406,765 - Pf6N2E2_3785 0.48 -0.2 4,406,928 + Pf6N2E2_3785 0.86 -0.2 4,406,928 + Pf6N2E2_3785 0.86 +1.8 4,406,928 + Pf6N2E2_3785 0.86 +0.1 4,406,928 + Pf6N2E2_3785 0.86 +0.2 4,406,928 + Pf6N2E2_3785 0.86 -0.9 4,406,928 + Pf6N2E2_3785 0.86 -0.4 4,406,928 + Pf6N2E2_3785 0.86 +0.3 4,406,962 + +0.6 4,406,962 + +1.0 4,406,962 + +0.6 4,407,292 + Pf6N2E2_3786 0.39 +0.1 4,407,370 + Pf6N2E2_3786 0.48 -2.5 4,407,370 + Pf6N2E2_3786 0.48 -0.7 4,407,373 + Pf6N2E2_3786 0.49 -3.1 4,407,454 + Pf6N2E2_3786 0.59 -1.5 4,407,454 + Pf6N2E2_3786 0.59 +0.3 4,407,556 + Pf6N2E2_3786 0.72 -1.7 4,407,577 + Pf6N2E2_3786 0.74 -2.7 4,407,577 + Pf6N2E2_3786 0.74 -2.2 4,407,577 + Pf6N2E2_3786 0.74 -0.9 4,408,491 + +1.7 4,408,491 + +0.1 4,408,491 + +2.1 4,408,491 + +0.3 4,408,491 + +0.9 4,408,491 + +1.5 4,408,492 - -0.1 4,408,492 - +1.2 4,408,558 - +0.7 4,408,576 + +0.2 4,408,577 - +0.8 4,408,591 + +1.2 4,408,591 + +1.8 4,408,591 + +0.8 4,408,592 - -1.7 4,408,592 - +0.5 4,408,611 + +0.8 4,408,611 + +0.1 4,408,618 + -0.8 4,408,619 - -0.1 4,408,619 - +0.6 4,408,619 - -0.5 4,408,619 - -0.5 4,408,619 - +1.6 4,408,620 + +0.8 4,408,621 - +0.2 4,408,638 - +0.6
Or see this region's nucleotide sequence