Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2372 and Pf6N2E2_2373 are separated by 4 nucleotides Pf6N2E2_2373 and Pf6N2E2_2374 are separated by 243 nucleotides
Pf6N2E2_2372 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 2,806,770 to 2,808,425
_2372
Pf6N2E2_2373 - Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid), at 2,808,430 to 2,809,167
_2373
Pf6N2E2_2374 - Nitrate ABC transporter, nitrate-binding protein, at 2,809,411 to 2,810,622
_2374
Position (kb)
2808
2809
2810 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2807.852 kb on - strand, within Pf6N2E2_2372 at 2807.971 kb on + strand, within Pf6N2E2_2372 at 2807.971 kb on + strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.154 kb on + strand, within Pf6N2E2_2372 at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.335 kb on - strand at 2808.377 kb on + strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.727 kb on + strand, within Pf6N2E2_2373 at 2808.736 kb on + strand, within Pf6N2E2_2373 at 2808.786 kb on - strand, within Pf6N2E2_2373 at 2809.183 kb on + strand at 2809.183 kb on + strand at 2809.184 kb on - strand at 2809.184 kb on - strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.188 kb on - strand at 2809.265 kb on + strand at 2809.265 kb on + strand at 2809.265 kb on + strand at 2809.266 kb on - strand at 2809.266 kb on - strand at 2809.266 kb on - strand at 2809.331 kb on - strand at 2809.657 kb on + strand, within Pf6N2E2_2374 at 2809.657 kb on + strand, within Pf6N2E2_2374 at 2809.658 kb on - strand, within Pf6N2E2_2374 at 2809.658 kb on - strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.870 kb on + strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.871 kb on - strand, within Pf6N2E2_2374 at 2809.873 kb on + strand, within Pf6N2E2_2374 at 2809.874 kb on - strand, within Pf6N2E2_2374 at 2809.969 kb on - strand, within Pf6N2E2_2374
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,807,852 - Pf6N2E2_2372 0.65 +0.1 2,807,971 + Pf6N2E2_2372 0.73 +0.3 2,807,971 + Pf6N2E2_2372 0.73 -1.7 2,807,972 - Pf6N2E2_2372 0.73 +0.3 2,807,972 - Pf6N2E2_2372 0.73 -0.8 2,807,972 - Pf6N2E2_2372 0.73 -0.3 2,808,128 - Pf6N2E2_2372 0.82 +0.1 2,808,128 - Pf6N2E2_2372 0.82 -2.1 2,808,128 - Pf6N2E2_2372 0.82 -0.2 2,808,133 + Pf6N2E2_2372 0.82 -0.6 2,808,133 + Pf6N2E2_2372 0.82 +0.6 2,808,133 + Pf6N2E2_2372 0.82 +0.6 2,808,133 + Pf6N2E2_2372 0.82 -0.7 2,808,133 + Pf6N2E2_2372 0.82 +0.9 2,808,134 - Pf6N2E2_2372 0.82 -0.5 2,808,134 - Pf6N2E2_2372 0.82 +0.4 2,808,134 - Pf6N2E2_2372 0.82 +0.8 2,808,154 + Pf6N2E2_2372 0.84 +0.3 2,808,334 + -0.9 2,808,334 + +0.3 2,808,334 + +0.0 2,808,334 + +0.1 2,808,335 - +0.9 2,808,377 + +1.1 2,808,447 - +0.2 2,808,447 - +0.6 2,808,447 - +1.8 2,808,447 - -1.1 2,808,727 + Pf6N2E2_2373 0.40 -1.3 2,808,736 + Pf6N2E2_2373 0.41 +0.2 2,808,786 - Pf6N2E2_2373 0.48 -0.9 2,809,183 + +0.7 2,809,183 + -1.4 2,809,184 - +0.4 2,809,184 - +2.1 2,809,187 + +0.5 2,809,187 + -0.1 2,809,187 + -0.1 2,809,187 + -0.2 2,809,187 + +0.9 2,809,188 - -0.0 2,809,265 + +0.5 2,809,265 + +2.3 2,809,265 + +0.4 2,809,266 - +0.5 2,809,266 - +0.2 2,809,266 - -0.9 2,809,331 - +0.4 2,809,657 + Pf6N2E2_2374 0.20 +0.5 2,809,657 + Pf6N2E2_2374 0.20 +0.7 2,809,658 - Pf6N2E2_2374 0.20 -0.9 2,809,658 - Pf6N2E2_2374 0.20 +0.4 2,809,870 + Pf6N2E2_2374 0.38 +0.2 2,809,870 + Pf6N2E2_2374 0.38 +0.1 2,809,870 + Pf6N2E2_2374 0.38 +0.2 2,809,870 + Pf6N2E2_2374 0.38 +0.9 2,809,870 + Pf6N2E2_2374 0.38 +0.2 2,809,870 + Pf6N2E2_2374 0.38 -0.2 2,809,871 - Pf6N2E2_2374 0.38 +0.4 2,809,871 - Pf6N2E2_2374 0.38 -0.2 2,809,871 - Pf6N2E2_2374 0.38 -0.3 2,809,871 - Pf6N2E2_2374 0.38 -0.7 2,809,873 + Pf6N2E2_2374 0.38 +1.1 2,809,874 - Pf6N2E2_2374 0.38 -0.4 2,809,969 - Pf6N2E2_2374 0.46 +0.3
Or see this region's nucleotide sequence