Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_9 and Pf6N2E2_10 are separated by 37 nucleotides Pf6N2E2_10 and Pf6N2E2_11 are separated by 353 nucleotides Pf6N2E2_11 and Pf6N2E2_12 are separated by 144 nucleotides
Pf6N2E2_9 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 34,541 to 38,686
_9
Pf6N2E2_10 - PvdE, pyoverdine ABC export system, fused ATPase and permease components, at 38,724 to 40,382
_10
Pf6N2E2_11 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 40,736 to 40,888
_11
Pf6N2E2_12 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 41,033 to 41,908
_12
Position (kb)
38
39
40
41 Strain fitness (log2 ratio)
-1
0
1
2 at 37.733 kb on + strand, within Pf6N2E2_9 at 37.919 kb on + strand, within Pf6N2E2_9 at 37.919 kb on + strand, within Pf6N2E2_9 at 37.920 kb on - strand, within Pf6N2E2_9 at 37.934 kb on - strand, within Pf6N2E2_9 at 37.934 kb on - strand, within Pf6N2E2_9 at 38.049 kb on + strand, within Pf6N2E2_9 at 38.049 kb on + strand, within Pf6N2E2_9 at 38.073 kb on + strand, within Pf6N2E2_9 at 38.073 kb on + strand, within Pf6N2E2_9 at 38.074 kb on - strand, within Pf6N2E2_9 at 38.082 kb on + strand, within Pf6N2E2_9 at 38.083 kb on - strand, within Pf6N2E2_9 at 38.527 kb on - strand at 38.585 kb on + strand at 38.672 kb on + strand at 38.673 kb on - strand at 38.804 kb on - strand at 38.870 kb on + strand at 38.872 kb on + strand at 38.872 kb on + strand at 38.873 kb on - strand at 39.113 kb on - strand, within Pf6N2E2_10 at 39.115 kb on + strand, within Pf6N2E2_10 at 39.215 kb on - strand, within Pf6N2E2_10 at 39.538 kb on + strand, within Pf6N2E2_10 at 39.538 kb on + strand, within Pf6N2E2_10 at 39.538 kb on + strand, within Pf6N2E2_10 at 39.701 kb on - strand, within Pf6N2E2_10 at 39.794 kb on - strand, within Pf6N2E2_10 at 39.865 kb on + strand, within Pf6N2E2_10 at 39.865 kb on + strand, within Pf6N2E2_10 at 39.866 kb on - strand, within Pf6N2E2_10 at 40.106 kb on + strand, within Pf6N2E2_10 at 40.106 kb on + strand, within Pf6N2E2_10 at 40.106 kb on + strand, within Pf6N2E2_10 at 40.106 kb on + strand, within Pf6N2E2_10 at 40.106 kb on + strand, within Pf6N2E2_10 at 40.311 kb on + strand at 40.311 kb on + strand at 40.312 kb on - strand at 40.481 kb on + strand at 40.481 kb on + strand at 40.532 kb on + strand at 40.532 kb on + strand at 40.533 kb on - strand at 40.575 kb on - strand at 40.575 kb on - strand at 40.575 kb on - strand at 40.737 kb on + strand at 40.737 kb on + strand at 40.737 kb on + strand at 40.737 kb on + strand at 40.737 kb on + strand at 40.737 kb on + strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.738 kb on - strand at 40.742 kb on - strand at 40.742 kb on - strand at 40.911 kb on + strand at 40.912 kb on - strand at 40.912 kb on - strand at 40.912 kb on - strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.038 kb on + strand at 41.208 kb on + strand, within Pf6N2E2_12 at 41.209 kb on - strand, within Pf6N2E2_12 at 41.209 kb on - strand, within Pf6N2E2_12 at 41.221 kb on - strand, within Pf6N2E2_12 at 41.221 kb on - strand, within Pf6N2E2_12 at 41.241 kb on + strand, within Pf6N2E2_12 at 41.262 kb on + strand, within Pf6N2E2_12 at 41.331 kb on + strand, within Pf6N2E2_12 at 41.359 kb on - strand, within Pf6N2E2_12
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 37,733 + Pf6N2E2_9 0.77 +0.5 37,919 + Pf6N2E2_9 0.81 +0.3 37,919 + Pf6N2E2_9 0.81 -0.3 37,920 - Pf6N2E2_9 0.82 -1.1 37,934 - Pf6N2E2_9 0.82 +0.1 37,934 - Pf6N2E2_9 0.82 -0.6 38,049 + Pf6N2E2_9 0.85 +0.9 38,049 + Pf6N2E2_9 0.85 -0.5 38,073 + Pf6N2E2_9 0.85 +0.7 38,073 + Pf6N2E2_9 0.85 -1.2 38,074 - Pf6N2E2_9 0.85 +0.5 38,082 + Pf6N2E2_9 0.85 +0.8 38,083 - Pf6N2E2_9 0.85 +0.4 38,527 - -0.8 38,585 + +0.3 38,672 + +2.4 38,673 - +0.9 38,804 - +0.1 38,870 + +1.0 38,872 + +0.8 38,872 + -0.0 38,873 - +0.8 39,113 - Pf6N2E2_10 0.23 +0.6 39,115 + Pf6N2E2_10 0.24 +0.5 39,215 - Pf6N2E2_10 0.30 +0.5 39,538 + Pf6N2E2_10 0.49 +0.6 39,538 + Pf6N2E2_10 0.49 +0.6 39,538 + Pf6N2E2_10 0.49 +0.5 39,701 - Pf6N2E2_10 0.59 +0.2 39,794 - Pf6N2E2_10 0.64 +0.6 39,865 + Pf6N2E2_10 0.69 +1.2 39,865 + Pf6N2E2_10 0.69 -0.5 39,866 - Pf6N2E2_10 0.69 +1.1 40,106 + Pf6N2E2_10 0.83 +0.4 40,106 + Pf6N2E2_10 0.83 +0.9 40,106 + Pf6N2E2_10 0.83 +0.6 40,106 + Pf6N2E2_10 0.83 +1.1 40,106 + Pf6N2E2_10 0.83 +0.6 40,311 + -0.2 40,311 + +0.1 40,312 - +0.6 40,481 + -1.2 40,481 + +0.4 40,532 + +0.6 40,532 + -0.0 40,533 - +1.0 40,575 - -0.6 40,575 - -0.9 40,575 - +0.4 40,737 + -0.3 40,737 + +1.9 40,737 + -0.5 40,737 + -0.4 40,737 + -0.2 40,737 + +0.8 40,738 - +0.2 40,738 - -1.0 40,738 - +2.1 40,738 - -0.7 40,738 - +0.7 40,738 - -0.2 40,738 - +0.3 40,738 - -1.5 40,742 - -0.6 40,742 - +0.5 40,911 + +0.1 40,912 - +0.5 40,912 - -1.1 40,912 - +0.2 41,034 + +0.1 41,034 + -0.9 41,034 + +0.1 41,034 + +0.6 41,034 + +0.7 41,035 - +0.4 41,035 - -0.5 41,035 - -1.6 41,035 - +0.4 41,035 - +0.6 41,035 - +0.6 41,035 - +0.4 41,035 - -0.1 41,035 - +0.1 41,035 - +0.9 41,035 - +0.3 41,038 + +0.8 41,208 + Pf6N2E2_12 0.20 -0.0 41,209 - Pf6N2E2_12 0.20 -0.7 41,209 - Pf6N2E2_12 0.20 -0.1 41,221 - Pf6N2E2_12 0.21 -0.6 41,221 - Pf6N2E2_12 0.21 -0.1 41,241 + Pf6N2E2_12 0.24 +0.8 41,262 + Pf6N2E2_12 0.26 +0.5 41,331 + Pf6N2E2_12 0.34 +0.0 41,359 - Pf6N2E2_12 0.37 +1.5
Or see this region's nucleotide sequence