Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_544

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_543 and Pf1N1B4_544 are separated by 193 nucleotidesPf1N1B4_544 and Pf1N1B4_545 are separated by 105 nucleotides Pf1N1B4_543 - ATP-dependent RNA helicase SrmB, at 639,950 to 641,296 _543 Pf1N1B4_544 - Small-conductance mechanosensitive channel, at 641,490 to 642,788 _544 Pf1N1B4_545 - MadN protein, at 642,894 to 643,760 _545 Position (kb) 641 642 643Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 641.317 kb on - strandat 641.335 kb on - strandat 641.372 kb on - strandat 641.373 kb on + strandat 641.373 kb on + strandat 641.440 kb on + strandat 641.440 kb on + strandat 641.440 kb on + strandat 641.441 kb on - strandat 641.441 kb on - strandat 641.441 kb on - strandat 641.602 kb on + strandat 641.603 kb on - strandat 641.786 kb on + strand, within Pf1N1B4_544at 641.874 kb on + strand, within Pf1N1B4_544at 642.378 kb on + strand, within Pf1N1B4_544at 642.501 kb on + strandat 642.502 kb on - strand, within Pf1N1B4_544at 642.591 kb on + strand, within Pf1N1B4_544at 642.591 kb on + strand, within Pf1N1B4_544at 642.592 kb on - strand, within Pf1N1B4_544at 642.786 kb on + strandat 642.786 kb on + strandat 642.786 kb on + strandat 642.786 kb on + strandat 642.786 kb on + strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.787 kb on - strandat 642.907 kb on - strandat 642.907 kb on - strandat 642.932 kb on + strandat 643.099 kb on + strand, within Pf1N1B4_545at 643.228 kb on + strand, within Pf1N1B4_545at 643.228 kb on + strand, within Pf1N1B4_545at 643.228 kb on + strand, within Pf1N1B4_545at 643.229 kb on - strand, within Pf1N1B4_545at 643.229 kb on - strand, within Pf1N1B4_545at 643.229 kb on - strand, within Pf1N1B4_545at 643.229 kb on - strand, within Pf1N1B4_545at 643.229 kb on - strand, within Pf1N1B4_545at 643.235 kb on - strand, within Pf1N1B4_545at 643.291 kb on + strand, within Pf1N1B4_545at 643.291 kb on + strand, within Pf1N1B4_545at 643.292 kb on - strand, within Pf1N1B4_545at 643.534 kb on + strand, within Pf1N1B4_545at 643.547 kb on - strand, within Pf1N1B4_545at 643.547 kb on - strand, within Pf1N1B4_545at 643.547 kb on - strand, within Pf1N1B4_545at 643.547 kb on - strandat 643.675 kb on + strandat 643.676 kb on - strandat 643.676 kb on - strandat 643.700 kb on - strandat 643.700 kb on - strandat 643.749 kb on + strandat 643.750 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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641,317 - +0.4
641,335 - -1.4
641,372 - +1.1
641,373 + +1.7
641,373 + -0.3
641,440 + +1.1
641,440 + +0.7
641,440 + +0.1
641,441 - -2.9
641,441 - +0.9
641,441 - +0.8
641,602 + -0.3
641,603 - -1.0
641,786 + Pf1N1B4_544 0.23 -0.3
641,874 + Pf1N1B4_544 0.30 -1.7
642,378 + Pf1N1B4_544 0.68 -1.2
642,501 + +0.7
642,502 - Pf1N1B4_544 0.78 -0.1
642,591 + Pf1N1B4_544 0.85 +1.1
642,591 + Pf1N1B4_544 0.85 -0.7
642,592 - Pf1N1B4_544 0.85 -2.2
642,786 + -0.3
642,786 + -0.9
642,786 + -0.2
642,786 + -0.1
642,786 + +0.4
642,787 - -0.9
642,787 - +1.1
642,787 - -1.9
642,787 - -0.1
642,787 - +0.4
642,787 - -2.2
642,787 - +2.1
642,787 - -1.4
642,907 - -0.7
642,907 - -0.6
642,932 + +0.4
643,099 + Pf1N1B4_545 0.24 +0.8
643,228 + Pf1N1B4_545 0.39 -0.4
643,228 + Pf1N1B4_545 0.39 -1.9
643,228 + Pf1N1B4_545 0.39 +0.3
643,229 - Pf1N1B4_545 0.39 +0.8
643,229 - Pf1N1B4_545 0.39 +0.8
643,229 - Pf1N1B4_545 0.39 -0.4
643,229 - Pf1N1B4_545 0.39 -1.0
643,229 - Pf1N1B4_545 0.39 +0.1
643,235 - Pf1N1B4_545 0.39 +1.1
643,291 + Pf1N1B4_545 0.46 -0.2
643,291 + Pf1N1B4_545 0.46 -0.6
643,292 - Pf1N1B4_545 0.46 +0.9
643,534 + Pf1N1B4_545 0.74 +0.3
643,547 - Pf1N1B4_545 0.75 +0.8
643,547 - Pf1N1B4_545 0.75 +0.6
643,547 - Pf1N1B4_545 0.75 -0.9
643,547 - +2.1
643,675 + +0.8
643,676 - -0.2
643,676 - -0.1
643,700 - +0.6
643,700 - -1.2
643,749 + -0.4
643,750 - -0.1

Or see this region's nucleotide sequence