Experiment: D-Galactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1109 and Pf1N1B4_1110 are separated by 59 nucleotides Pf1N1B4_1110 and Pf1N1B4_1111 are separated by 235 nucleotides
Pf1N1B4_1109 - 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (from data) , at 1,242,992 to 1,244,437
_1109
Pf1N1B4_1110 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) (from data) , at 1,244,497 to 1,245,408
_1110
Pf1N1B4_1111 - Phosphatidylcholine-hydrolyzing phospholipase C (EC 3.1.4.3), at 1,245,644 to 1,246,963
_1111
Position (kb)
1244
1245
1246 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1243.605 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.065 kb on - strand, within Pf1N1B4_1109 at 1244.065 kb on - strand, within Pf1N1B4_1109 at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.498 kb on + strand at 1244.540 kb on + strand at 1244.591 kb on + strand, within Pf1N1B4_1110 at 1244.591 kb on + strand, within Pf1N1B4_1110 at 1244.592 kb on - strand, within Pf1N1B4_1110 at 1244.592 kb on - strand, within Pf1N1B4_1110 at 1244.592 kb on - strand, within Pf1N1B4_1110 at 1244.777 kb on + strand, within Pf1N1B4_1110 at 1244.778 kb on - strand, within Pf1N1B4_1110 at 1244.814 kb on - strand at 1244.843 kb on + strand, within Pf1N1B4_1110 at 1244.844 kb on - strand, within Pf1N1B4_1110 at 1244.988 kb on - strand, within Pf1N1B4_1110 at 1244.988 kb on - strand, within Pf1N1B4_1110 at 1244.988 kb on - strand, within Pf1N1B4_1110 at 1245.008 kb on - strand, within Pf1N1B4_1110 at 1245.068 kb on + strand, within Pf1N1B4_1110 at 1245.069 kb on - strand, within Pf1N1B4_1110 at 1245.069 kb on - strand, within Pf1N1B4_1110 at 1245.122 kb on + strand, within Pf1N1B4_1110 at 1245.123 kb on - strand, within Pf1N1B4_1110 at 1245.152 kb on - strand, within Pf1N1B4_1110 at 1245.311 kb on + strand, within Pf1N1B4_1110 at 1245.311 kb on + strand, within Pf1N1B4_1110 at 1245.427 kb on - strand at 1245.492 kb on + strand at 1245.546 kb on - strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.595 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.618 kb on - strand at 1245.621 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.639 kb on + strand at 1245.640 kb on - strand at 1245.640 kb on - strand at 1245.654 kb on + strand at 1245.656 kb on + strand at 1245.677 kb on - strand at 1245.928 kb on - strand, within Pf1N1B4_1111 at 1245.928 kb on - strand, within Pf1N1B4_1111 at 1245.928 kb on - strand at 1245.940 kb on - strand, within Pf1N1B4_1111 at 1245.962 kb on + strand, within Pf1N1B4_1111 at 1245.962 kb on + strand at 1245.963 kb on - strand, within Pf1N1B4_1111 at 1246.058 kb on - strand, within Pf1N1B4_1111 at 1246.190 kb on + strand, within Pf1N1B4_1111 at 1246.191 kb on - strand, within Pf1N1B4_1111 at 1246.191 kb on - strand at 1246.207 kb on + strand, within Pf1N1B4_1111 at 1246.207 kb on + strand, within Pf1N1B4_1111 at 1246.208 kb on - strand, within Pf1N1B4_1111 at 1246.208 kb on - strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.338 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose (C) remove 1,243,605 + Pf1N1B4_1109 0.42 -0.1 1,244,064 + Pf1N1B4_1109 0.74 -0.8 1,244,064 + Pf1N1B4_1109 0.74 +1.7 1,244,064 + Pf1N1B4_1109 0.74 +0.1 1,244,064 + Pf1N1B4_1109 0.74 +0.0 1,244,064 + Pf1N1B4_1109 0.74 +0.6 1,244,065 - Pf1N1B4_1109 0.74 -0.7 1,244,065 - Pf1N1B4_1109 0.74 -2.1 1,244,409 + +0.8 1,244,409 + -0.2 1,244,409 + -0.7 1,244,409 + -2.1 1,244,409 + +0.6 1,244,409 + -0.2 1,244,409 + -0.5 1,244,409 + -0.2 1,244,409 + +0.5 1,244,409 + -0.2 1,244,409 + +1.3 1,244,409 + +0.2 1,244,410 - +0.3 1,244,410 - -0.3 1,244,410 - +0.3 1,244,410 - -1.5 1,244,410 - +0.7 1,244,410 - +0.3 1,244,410 - +0.3 1,244,498 + -0.2 1,244,540 + +0.1 1,244,591 + Pf1N1B4_1110 0.10 +0.2 1,244,591 + Pf1N1B4_1110 0.10 -0.1 1,244,592 - Pf1N1B4_1110 0.10 +0.0 1,244,592 - Pf1N1B4_1110 0.10 -1.1 1,244,592 - Pf1N1B4_1110 0.10 -2.2 1,244,777 + Pf1N1B4_1110 0.31 +2.3 1,244,778 - Pf1N1B4_1110 0.31 -0.7 1,244,814 - +0.3 1,244,843 + Pf1N1B4_1110 0.38 -1.0 1,244,844 - Pf1N1B4_1110 0.38 +0.3 1,244,988 - Pf1N1B4_1110 0.54 +0.3 1,244,988 - Pf1N1B4_1110 0.54 +0.3 1,244,988 - Pf1N1B4_1110 0.54 -1.5 1,245,008 - Pf1N1B4_1110 0.56 -1.7 1,245,068 + Pf1N1B4_1110 0.63 -0.5 1,245,069 - Pf1N1B4_1110 0.63 -0.4 1,245,069 - Pf1N1B4_1110 0.63 -1.5 1,245,122 + Pf1N1B4_1110 0.69 +0.4 1,245,123 - Pf1N1B4_1110 0.69 -0.8 1,245,152 - Pf1N1B4_1110 0.72 +0.2 1,245,311 + Pf1N1B4_1110 0.89 -0.2 1,245,311 + Pf1N1B4_1110 0.89 +2.3 1,245,427 - -0.2 1,245,492 + -0.0 1,245,546 - -1.5 1,245,574 + -0.9 1,245,574 + -0.2 1,245,574 + +0.8 1,245,574 + -0.2 1,245,574 + -1.2 1,245,574 + +1.3 1,245,575 - +0.1 1,245,575 - +0.9 1,245,575 - +1.0 1,245,575 - -0.2 1,245,575 - +0.9 1,245,575 - +1.1 1,245,575 - +0.5 1,245,595 + +0.5 1,245,617 + +0.2 1,245,617 + -0.9 1,245,618 - -0.2 1,245,621 + -1.0 1,245,628 + -1.2 1,245,628 + +0.2 1,245,628 + +1.3 1,245,628 + -0.2 1,245,628 + -1.0 1,245,628 + +0.8 1,245,628 + +0.5 1,245,629 - -0.7 1,245,629 - -1.1 1,245,629 - -0.7 1,245,629 - +0.9 1,245,629 - +0.2 1,245,639 + -2.2 1,245,640 - -0.2 1,245,640 - -1.0 1,245,654 + -1.2 1,245,656 + +0.2 1,245,677 - -0.2 1,245,928 - Pf1N1B4_1111 0.22 -0.2 1,245,928 - Pf1N1B4_1111 0.22 +1.1 1,245,928 - -0.5 1,245,940 - Pf1N1B4_1111 0.22 +0.5 1,245,962 + Pf1N1B4_1111 0.24 +0.3 1,245,962 + +2.9 1,245,963 - Pf1N1B4_1111 0.24 -1.1 1,246,058 - Pf1N1B4_1111 0.31 +0.1 1,246,190 + Pf1N1B4_1111 0.41 +0.1 1,246,191 - Pf1N1B4_1111 0.41 +0.0 1,246,191 - +1.9 1,246,207 + Pf1N1B4_1111 0.43 +0.0 1,246,207 + Pf1N1B4_1111 0.43 -1.5 1,246,208 - Pf1N1B4_1111 0.43 -0.2 1,246,208 - Pf1N1B4_1111 0.43 +0.3 1,246,337 + Pf1N1B4_1111 0.53 -0.7 1,246,337 + Pf1N1B4_1111 0.53 -0.4 1,246,337 + Pf1N1B4_1111 0.53 -0.5 1,246,337 + Pf1N1B4_1111 0.53 -1.8 1,246,337 + Pf1N1B4_1111 0.53 +0.3 1,246,338 - +0.3
Or see this region's nucleotide sequence