Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25240 and CA265_RS25245 are separated by 63 nucleotides CA265_RS25245 and CA265_RS25250 are separated by 23 nucleotides CA265_RS25250 and CA265_RS25255 are separated by 17 nucleotides CA265_RS25255 and CA265_RS25260 overlap by 20 nucleotides CA265_RS25260 and CA265_RS25265 are separated by 209 nucleotides
CA265_RS25240: CA265_RS25240 - hypothetical protein, at 6,143,831 to 6,144,238
_RS25240
CA265_RS25245: CA265_RS25245 - hypothetical protein, at 6,144,302 to 6,144,865
_RS25245
CA265_RS25250: CA265_RS25250 - hypothetical protein, at 6,144,889 to 6,145,773
_RS25250
CA265_RS25255: CA265_RS25255 - methyltransferase type 11, at 6,145,791 to 6,146,225
_RS25255
CA265_RS25260: CA265_RS25260 - hypothetical protein, at 6,146,206 to 6,146,526
_RS25260
CA265_RS25265: CA265_RS25265 - hypothetical protein, at 6,146,736 to 6,147,014
_RS25265
Position (kb)
6144
6145
6146 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6143.925 kb on - strand, within CA265_RS25240 at 6143.925 kb on - strand, within CA265_RS25240 at 6143.926 kb on + strand, within CA265_RS25240 at 6143.927 kb on - strand, within CA265_RS25240 at 6143.932 kb on - strand, within CA265_RS25240 at 6143.963 kb on - strand, within CA265_RS25240 at 6144.016 kb on + strand, within CA265_RS25240 at 6144.076 kb on + strand, within CA265_RS25240 at 6144.172 kb on - strand, within CA265_RS25240 at 6144.236 kb on + strand at 6144.314 kb on - strand at 6144.318 kb on + strand at 6144.324 kb on - strand at 6144.392 kb on + strand, within CA265_RS25245 at 6144.407 kb on + strand, within CA265_RS25245 at 6144.408 kb on - strand, within CA265_RS25245 at 6144.429 kb on + strand, within CA265_RS25245 at 6144.430 kb on - strand, within CA265_RS25245 at 6144.430 kb on - strand, within CA265_RS25245 at 6144.451 kb on + strand, within CA265_RS25245 at 6144.452 kb on - strand, within CA265_RS25245 at 6144.461 kb on - strand, within CA265_RS25245 at 6144.461 kb on - strand, within CA265_RS25245 at 6144.515 kb on + strand, within CA265_RS25245 at 6144.516 kb on - strand, within CA265_RS25245 at 6144.529 kb on - strand, within CA265_RS25245 at 6144.586 kb on - strand, within CA265_RS25245 at 6144.607 kb on + strand, within CA265_RS25245 at 6144.749 kb on - strand, within CA265_RS25245 at 6144.753 kb on + strand, within CA265_RS25245 at 6144.754 kb on - strand, within CA265_RS25245 at 6144.759 kb on + strand, within CA265_RS25245 at 6144.762 kb on + strand, within CA265_RS25245 at 6144.762 kb on + strand, within CA265_RS25245 at 6144.763 kb on - strand, within CA265_RS25245 at 6144.763 kb on - strand, within CA265_RS25245 at 6144.826 kb on - strand at 6144.826 kb on - strand at 6144.829 kb on - strand at 6144.829 kb on - strand at 6144.869 kb on - strand at 6144.933 kb on - strand at 6145.065 kb on + strand, within CA265_RS25250 at 6145.101 kb on + strand, within CA265_RS25250 at 6145.102 kb on - strand, within CA265_RS25250 at 6145.198 kb on - strand, within CA265_RS25250 at 6145.232 kb on - strand, within CA265_RS25250 at 6145.354 kb on - strand, within CA265_RS25250 at 6145.391 kb on + strand, within CA265_RS25250 at 6145.391 kb on + strand, within CA265_RS25250 at 6145.392 kb on - strand, within CA265_RS25250 at 6145.424 kb on + strand, within CA265_RS25250 at 6145.428 kb on + strand, within CA265_RS25250 at 6145.429 kb on - strand, within CA265_RS25250 at 6145.429 kb on - strand, within CA265_RS25250 at 6145.448 kb on + strand, within CA265_RS25250 at 6145.448 kb on + strand, within CA265_RS25250 at 6145.474 kb on + strand, within CA265_RS25250 at 6145.475 kb on - strand, within CA265_RS25250 at 6145.572 kb on + strand, within CA265_RS25250 at 6145.603 kb on + strand, within CA265_RS25250 at 6145.646 kb on + strand, within CA265_RS25250 at 6145.647 kb on - strand, within CA265_RS25250 at 6145.654 kb on + strand, within CA265_RS25250 at 6145.654 kb on + strand, within CA265_RS25250 at 6145.655 kb on - strand, within CA265_RS25250 at 6145.655 kb on - strand, within CA265_RS25250 at 6145.718 kb on + strand at 6145.739 kb on - strand at 6145.753 kb on + strand at 6145.790 kb on + strand at 6145.814 kb on - strand at 6145.814 kb on - strand at 6145.859 kb on - strand, within CA265_RS25255 at 6145.859 kb on - strand, within CA265_RS25255 at 6145.889 kb on + strand, within CA265_RS25255 at 6145.919 kb on + strand, within CA265_RS25255 at 6145.931 kb on - strand, within CA265_RS25255 at 6145.969 kb on + strand, within CA265_RS25255 at 6145.981 kb on - strand, within CA265_RS25255 at 6146.019 kb on - strand, within CA265_RS25255 at 6146.019 kb on - strand, within CA265_RS25255 at 6146.019 kb on - strand, within CA265_RS25255 at 6146.022 kb on - strand, within CA265_RS25255 at 6146.033 kb on - strand, within CA265_RS25255 at 6146.069 kb on - strand, within CA265_RS25255 at 6146.069 kb on - strand, within CA265_RS25255 at 6146.112 kb on + strand, within CA265_RS25255 at 6146.113 kb on - strand, within CA265_RS25255 at 6146.113 kb on - strand, within CA265_RS25255 at 6146.113 kb on - strand, within CA265_RS25255 at 6146.115 kb on - strand, within CA265_RS25255 at 6146.115 kb on - strand, within CA265_RS25255 at 6146.131 kb on - strand, within CA265_RS25255 at 6146.133 kb on - strand, within CA265_RS25255 at 6146.133 kb on - strand, within CA265_RS25255 at 6146.133 kb on - strand, within CA265_RS25255 at 6146.141 kb on - strand, within CA265_RS25255 at 6146.167 kb on + strand, within CA265_RS25255 at 6146.168 kb on - strand, within CA265_RS25255 at 6146.203 kb on + strand at 6146.321 kb on + strand, within CA265_RS25260 at 6146.341 kb on - strand, within CA265_RS25260 at 6146.362 kb on + strand, within CA265_RS25260 at 6146.363 kb on - strand, within CA265_RS25260 at 6146.407 kb on - strand, within CA265_RS25260 at 6146.491 kb on + strand, within CA265_RS25260 at 6146.505 kb on - strand at 6146.506 kb on + strand at 6146.506 kb on + strand at 6146.528 kb on + strand at 6146.528 kb on + strand at 6146.530 kb on + strand at 6146.530 kb on + strand at 6146.535 kb on - strand at 6146.567 kb on + strand at 6146.624 kb on - strand at 6146.624 kb on - strand at 6146.624 kb on - strand at 6146.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 6,143,925 - CA265_RS25240 0.23 -1.9 6,143,925 - CA265_RS25240 0.23 -0.1 6,143,926 + CA265_RS25240 0.23 +0.2 6,143,927 - CA265_RS25240 0.24 +0.8 6,143,932 - CA265_RS25240 0.25 +0.6 6,143,963 - CA265_RS25240 0.32 +0.3 6,144,016 + CA265_RS25240 0.45 +0.8 6,144,076 + CA265_RS25240 0.60 +0.4 6,144,172 - CA265_RS25240 0.84 -0.3 6,144,236 + +0.4 6,144,314 - +1.5 6,144,318 + +0.4 6,144,324 - -0.0 6,144,392 + CA265_RS25245 0.16 -0.5 6,144,407 + CA265_RS25245 0.19 +0.4 6,144,408 - CA265_RS25245 0.19 -0.1 6,144,429 + CA265_RS25245 0.23 -0.5 6,144,430 - CA265_RS25245 0.23 -0.6 6,144,430 - CA265_RS25245 0.23 +0.3 6,144,451 + CA265_RS25245 0.26 +0.1 6,144,452 - CA265_RS25245 0.27 -1.2 6,144,461 - CA265_RS25245 0.28 +0.6 6,144,461 - CA265_RS25245 0.28 +0.9 6,144,515 + CA265_RS25245 0.38 -0.2 6,144,516 - CA265_RS25245 0.38 -0.2 6,144,529 - CA265_RS25245 0.40 +0.3 6,144,586 - CA265_RS25245 0.50 -0.6 6,144,607 + CA265_RS25245 0.54 -0.3 6,144,749 - CA265_RS25245 0.79 +0.2 6,144,753 + CA265_RS25245 0.80 -0.9 6,144,754 - CA265_RS25245 0.80 -1.1 6,144,759 + CA265_RS25245 0.81 -1.0 6,144,762 + CA265_RS25245 0.82 +0.3 6,144,762 + CA265_RS25245 0.82 -0.3 6,144,763 - CA265_RS25245 0.82 -0.3 6,144,763 - CA265_RS25245 0.82 +0.1 6,144,826 - -0.1 6,144,826 - +0.4 6,144,829 - -0.1 6,144,829 - -0.6 6,144,869 - +0.1 6,144,933 - -0.3 6,145,065 + CA265_RS25250 0.20 -0.3 6,145,101 + CA265_RS25250 0.24 -1.6 6,145,102 - CA265_RS25250 0.24 -0.6 6,145,198 - CA265_RS25250 0.35 -1.2 6,145,232 - CA265_RS25250 0.39 -1.1 6,145,354 - CA265_RS25250 0.53 -0.8 6,145,391 + CA265_RS25250 0.57 +0.2 6,145,391 + CA265_RS25250 0.57 +0.4 6,145,392 - CA265_RS25250 0.57 -1.1 6,145,424 + CA265_RS25250 0.60 -0.7 6,145,428 + CA265_RS25250 0.61 -1.3 6,145,429 - CA265_RS25250 0.61 -0.3 6,145,429 - CA265_RS25250 0.61 -0.9 6,145,448 + CA265_RS25250 0.63 +0.5 6,145,448 + CA265_RS25250 0.63 +0.1 6,145,474 + CA265_RS25250 0.66 +0.2 6,145,475 - CA265_RS25250 0.66 -0.8 6,145,572 + CA265_RS25250 0.77 +0.7 6,145,603 + CA265_RS25250 0.81 -0.1 6,145,646 + CA265_RS25250 0.86 -0.6 6,145,647 - CA265_RS25250 0.86 -0.9 6,145,654 + CA265_RS25250 0.86 +1.4 6,145,654 + CA265_RS25250 0.86 +0.4 6,145,655 - CA265_RS25250 0.87 -2.1 6,145,655 - CA265_RS25250 0.87 -0.7 6,145,718 + +0.5 6,145,739 - -0.8 6,145,753 + -0.2 6,145,790 + +0.6 6,145,814 - -0.5 6,145,814 - -1.5 6,145,859 - CA265_RS25255 0.16 +0.3 6,145,859 - CA265_RS25255 0.16 -1.1 6,145,889 + CA265_RS25255 0.23 +0.8 6,145,919 + CA265_RS25255 0.29 +0.5 6,145,931 - CA265_RS25255 0.32 +0.2 6,145,969 + CA265_RS25255 0.41 +1.7 6,145,981 - CA265_RS25255 0.44 -0.8 6,146,019 - CA265_RS25255 0.52 -0.5 6,146,019 - CA265_RS25255 0.52 -1.2 6,146,019 - CA265_RS25255 0.52 +0.1 6,146,022 - CA265_RS25255 0.53 -0.9 6,146,033 - CA265_RS25255 0.56 -0.7 6,146,069 - CA265_RS25255 0.64 -1.0 6,146,069 - CA265_RS25255 0.64 -0.0 6,146,112 + CA265_RS25255 0.74 +0.3 6,146,113 - CA265_RS25255 0.74 -1.7 6,146,113 - CA265_RS25255 0.74 -2.5 6,146,113 - CA265_RS25255 0.74 +0.5 6,146,115 - CA265_RS25255 0.74 -0.7 6,146,115 - CA265_RS25255 0.74 +1.0 6,146,131 - CA265_RS25255 0.78 -0.6 6,146,133 - CA265_RS25255 0.79 -1.7 6,146,133 - CA265_RS25255 0.79 -2.8 6,146,133 - CA265_RS25255 0.79 +0.2 6,146,141 - CA265_RS25255 0.80 +0.4 6,146,167 + CA265_RS25255 0.86 -0.3 6,146,168 - CA265_RS25255 0.87 -0.3 6,146,203 + +0.4 6,146,321 + CA265_RS25260 0.36 -0.2 6,146,341 - CA265_RS25260 0.42 -1.1 6,146,362 + CA265_RS25260 0.49 +0.5 6,146,363 - CA265_RS25260 0.49 -0.8 6,146,407 - CA265_RS25260 0.63 -0.8 6,146,491 + CA265_RS25260 0.89 +1.1 6,146,505 - -0.7 6,146,506 + -0.2 6,146,506 + +0.9 6,146,528 + -0.4 6,146,528 + +0.8 6,146,530 + +0.8 6,146,530 + +0.9 6,146,535 - -0.0 6,146,567 + +0.5 6,146,624 - -0.8 6,146,624 - -0.6 6,146,624 - -0.3 6,146,627 + +1.3
Or see this region's nucleotide sequence