Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24765 and CA265_RS24770 are separated by 291 nucleotides CA265_RS24770 and CA265_RS24775 are separated by 296 nucleotides CA265_RS24775 and CA265_RS24780 are separated by 135 nucleotides CA265_RS24780 and CA265_RS24785 are separated by 241 nucleotides
CA265_RS24765: CA265_RS24765 - RNA-binding protein, at 6,046,551 to 6,046,841
_RS24765
CA265_RS24770: CA265_RS24770 - hypothetical protein, at 6,047,133 to 6,047,312
_RS24770
CA265_RS24775: CA265_RS24775 - peptidylprolyl isomerase, at 6,047,609 to 6,048,382
_RS24775
CA265_RS24780: CA265_RS24780 - nucleoside-diphosphate kinase, at 6,048,518 to 6,048,937
_RS24780
CA265_RS24785: CA265_RS24785 - DHH family phosphoesterase, at 6,049,179 to 6,050,189
_RS24785
Position (kb)
6047
6048
6049 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6046.839 kb on + strand at 6046.844 kb on - strand at 6046.856 kb on - strand at 6046.864 kb on + strand at 6046.910 kb on + strand at 6046.910 kb on + strand at 6047.062 kb on + strand at 6047.068 kb on + strand at 6047.068 kb on + strand at 6047.114 kb on + strand at 6047.193 kb on + strand, within CA265_RS24770 at 6047.194 kb on - strand, within CA265_RS24770 at 6047.198 kb on + strand, within CA265_RS24770 at 6047.199 kb on - strand at 6047.232 kb on - strand, within CA265_RS24770 at 6047.238 kb on + strand, within CA265_RS24770 at 6047.238 kb on + strand, within CA265_RS24770 at 6047.314 kb on + strand at 6047.316 kb on - strand at 6047.325 kb on + strand at 6047.329 kb on + strand at 6047.330 kb on - strand at 6047.414 kb on - strand at 6047.425 kb on + strand at 6047.425 kb on + strand at 6047.500 kb on + strand at 6047.500 kb on + strand at 6047.500 kb on + strand at 6047.500 kb on + strand at 6047.501 kb on - strand at 6047.503 kb on - strand at 6047.571 kb on - strand at 6047.658 kb on + strand at 6047.669 kb on - strand at 6047.750 kb on + strand, within CA265_RS24775 at 6047.855 kb on - strand, within CA265_RS24775 at 6047.920 kb on - strand, within CA265_RS24775 at 6047.995 kb on + strand, within CA265_RS24775 at 6048.076 kb on + strand, within CA265_RS24775 at 6048.112 kb on + strand, within CA265_RS24775 at 6048.112 kb on + strand, within CA265_RS24775 at 6048.113 kb on - strand, within CA265_RS24775 at 6048.150 kb on - strand, within CA265_RS24775 at 6048.224 kb on - strand, within CA265_RS24775 at 6048.230 kb on + strand, within CA265_RS24775 at 6048.231 kb on - strand, within CA265_RS24775 at 6048.231 kb on - strand, within CA265_RS24775 at 6048.254 kb on - strand, within CA265_RS24775 at 6048.354 kb on - strand at 6048.399 kb on - strand at 6048.399 kb on - strand at 6048.457 kb on + strand at 6048.458 kb on - strand at 6048.470 kb on - strand at 6048.551 kb on - strand at 6048.707 kb on + strand, within CA265_RS24780 at 6048.713 kb on - strand, within CA265_RS24780 at 6048.725 kb on - strand, within CA265_RS24780 at 6048.740 kb on - strand, within CA265_RS24780 at 6048.746 kb on + strand, within CA265_RS24780 at 6048.746 kb on + strand, within CA265_RS24780 at 6048.749 kb on + strand, within CA265_RS24780 at 6048.750 kb on - strand, within CA265_RS24780 at 6048.750 kb on - strand, within CA265_RS24780 at 6048.818 kb on - strand, within CA265_RS24780 at 6048.853 kb on + strand, within CA265_RS24780 at 6048.854 kb on - strand, within CA265_RS24780 at 6048.907 kb on + strand at 6048.994 kb on + strand at 6048.994 kb on + strand at 6049.029 kb on - strand at 6049.039 kb on + strand at 6049.039 kb on + strand at 6049.040 kb on - strand at 6049.045 kb on + strand at 6049.046 kb on - strand at 6049.076 kb on - strand at 6049.109 kb on - strand at 6049.109 kb on - strand at 6049.147 kb on - strand at 6049.156 kb on + strand at 6049.178 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 6,046,839 + +0.2 6,046,844 - +0.9 6,046,856 - -0.6 6,046,864 + -0.2 6,046,910 + +1.4 6,046,910 + +0.2 6,047,062 + -0.4 6,047,068 + -0.6 6,047,068 + +0.0 6,047,114 + -0.4 6,047,193 + CA265_RS24770 0.33 -0.5 6,047,194 - CA265_RS24770 0.34 +1.3 6,047,198 + CA265_RS24770 0.36 +1.5 6,047,199 - +0.4 6,047,232 - CA265_RS24770 0.55 -0.4 6,047,238 + CA265_RS24770 0.58 +1.4 6,047,238 + CA265_RS24770 0.58 +0.6 6,047,314 + +0.8 6,047,316 - +0.4 6,047,325 + +0.2 6,047,329 + +0.6 6,047,330 - +1.0 6,047,414 - +0.8 6,047,425 + -1.1 6,047,425 + -1.6 6,047,500 + +0.8 6,047,500 + +0.2 6,047,500 + +0.8 6,047,500 + -0.3 6,047,501 - -1.8 6,047,503 - -0.9 6,047,571 - +0.5 6,047,658 + +0.1 6,047,669 - +1.4 6,047,750 + CA265_RS24775 0.18 +0.1 6,047,855 - CA265_RS24775 0.32 +0.5 6,047,920 - CA265_RS24775 0.40 +0.5 6,047,995 + CA265_RS24775 0.50 -0.7 6,048,076 + CA265_RS24775 0.60 -1.2 6,048,112 + CA265_RS24775 0.65 +0.5 6,048,112 + CA265_RS24775 0.65 +1.0 6,048,113 - CA265_RS24775 0.65 +1.4 6,048,150 - CA265_RS24775 0.70 +0.7 6,048,224 - CA265_RS24775 0.79 +0.8 6,048,230 + CA265_RS24775 0.80 +0.8 6,048,231 - CA265_RS24775 0.80 +2.0 6,048,231 - CA265_RS24775 0.80 +0.5 6,048,254 - CA265_RS24775 0.83 +0.8 6,048,354 - +0.2 6,048,399 - -0.6 6,048,399 - -0.3 6,048,457 + -0.1 6,048,458 - -0.6 6,048,470 - +0.3 6,048,551 - -3.0 6,048,707 + CA265_RS24780 0.45 -0.5 6,048,713 - CA265_RS24780 0.46 +0.6 6,048,725 - CA265_RS24780 0.49 -1.2 6,048,740 - CA265_RS24780 0.53 -1.2 6,048,746 + CA265_RS24780 0.54 -0.3 6,048,746 + CA265_RS24780 0.54 +0.1 6,048,749 + CA265_RS24780 0.55 -1.9 6,048,750 - CA265_RS24780 0.55 -1.7 6,048,750 - CA265_RS24780 0.55 -1.6 6,048,818 - CA265_RS24780 0.71 +3.0 6,048,853 + CA265_RS24780 0.80 -2.0 6,048,854 - CA265_RS24780 0.80 -2.4 6,048,907 + -0.2 6,048,994 + +1.7 6,048,994 + +0.3 6,049,029 - +0.5 6,049,039 + +0.5 6,049,039 + +0.9 6,049,040 - +1.3 6,049,045 + +0.9 6,049,046 - +0.8 6,049,076 - -2.3 6,049,109 - +0.1 6,049,109 - -0.6 6,049,147 - +0.5 6,049,156 + -2.3 6,049,178 + +0.4
Or see this region's nucleotide sequence