Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24595 and CA265_RS24600 are separated by 206 nucleotides CA265_RS24600 and CA265_RS24605 are separated by 402 nucleotides CA265_RS24605 and CA265_RS24610 are separated by 149 nucleotides
CA265_RS24595: CA265_RS24595 - hypothetical protein, at 5,995,113 to 5,995,958
_RS24595
CA265_RS24600: CA265_RS24600 - glutaminase, at 5,996,165 to 5,998,639
_RS24600
CA265_RS24605: CA265_RS24605 - hypothetical protein, at 5,999,042 to 5,999,287
_RS24605
CA265_RS24610: CA265_RS24610 - DNA polymerase IV, at 5,999,437 to 6,000,537
_RS24610
Position (kb) 5996
5997
5998
5999 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5995.169 kb on - strand at 5995.308 kb on - strand, within CA265_RS24595 at 5995.314 kb on + strand, within CA265_RS24595 at 5995.324 kb on + strand, within CA265_RS24595 at 5995.349 kb on + strand, within CA265_RS24595 at 5995.350 kb on - strand, within CA265_RS24595 at 5995.376 kb on + strand, within CA265_RS24595 at 5995.380 kb on + strand, within CA265_RS24595 at 5995.381 kb on - strand, within CA265_RS24595 at 5995.381 kb on - strand, within CA265_RS24595 at 5995.399 kb on - strand, within CA265_RS24595 at 5995.400 kb on + strand, within CA265_RS24595 at 5995.507 kb on - strand, within CA265_RS24595 at 5995.507 kb on - strand, within CA265_RS24595 at 5995.638 kb on - strand, within CA265_RS24595 at 5995.650 kb on - strand, within CA265_RS24595 at 5995.661 kb on - strand, within CA265_RS24595 at 5995.665 kb on - strand, within CA265_RS24595 at 5995.679 kb on - strand, within CA265_RS24595 at 5995.679 kb on - strand, within CA265_RS24595 at 5995.686 kb on + strand, within CA265_RS24595 at 5995.686 kb on + strand, within CA265_RS24595 at 5995.686 kb on + strand, within CA265_RS24595 at 5995.687 kb on - strand, within CA265_RS24595 at 5995.768 kb on - strand, within CA265_RS24595 at 5995.782 kb on - strand, within CA265_RS24595 at 5995.782 kb on - strand, within CA265_RS24595 at 5995.869 kb on + strand, within CA265_RS24595 at 5995.893 kb on - strand at 5995.956 kb on + strand at 5995.956 kb on + strand at 5995.957 kb on - strand at 5995.957 kb on - strand at 5995.970 kb on + strand at 5995.989 kb on - strand at 5995.989 kb on - strand at 5996.090 kb on + strand at 5996.134 kb on + strand at 5996.164 kb on + strand at 5996.164 kb on + strand at 5996.165 kb on - strand at 5996.199 kb on + strand at 5996.270 kb on - strand at 5996.279 kb on - strand at 5996.279 kb on - strand at 5996.279 kb on - strand at 5996.282 kb on + strand at 5996.420 kb on + strand, within CA265_RS24600 at 5996.420 kb on + strand, within CA265_RS24600 at 5996.422 kb on + strand, within CA265_RS24600 at 5996.433 kb on + strand, within CA265_RS24600 at 5996.433 kb on + strand, within CA265_RS24600 at 5996.456 kb on + strand, within CA265_RS24600 at 5996.456 kb on + strand, within CA265_RS24600 at 5996.457 kb on - strand, within CA265_RS24600 at 5996.457 kb on - strand, within CA265_RS24600 at 5996.510 kb on + strand, within CA265_RS24600 at 5996.511 kb on - strand, within CA265_RS24600 at 5996.548 kb on - strand, within CA265_RS24600 at 5996.614 kb on + strand, within CA265_RS24600 at 5996.614 kb on + strand, within CA265_RS24600 at 5996.615 kb on - strand, within CA265_RS24600 at 5996.617 kb on + strand, within CA265_RS24600 at 5996.618 kb on - strand, within CA265_RS24600 at 5996.671 kb on + strand, within CA265_RS24600 at 5996.674 kb on + strand, within CA265_RS24600 at 5996.728 kb on + strand, within CA265_RS24600 at 5996.738 kb on + strand, within CA265_RS24600 at 5996.739 kb on - strand, within CA265_RS24600 at 5996.739 kb on - strand, within CA265_RS24600 at 5996.791 kb on - strand, within CA265_RS24600 at 5996.805 kb on + strand, within CA265_RS24600 at 5996.806 kb on - strand, within CA265_RS24600 at 5996.806 kb on - strand, within CA265_RS24600 at 5996.848 kb on - strand, within CA265_RS24600 at 5996.905 kb on + strand, within CA265_RS24600 at 5996.906 kb on - strand, within CA265_RS24600 at 5996.908 kb on + strand, within CA265_RS24600 at 5996.927 kb on + strand, within CA265_RS24600 at 5996.928 kb on - strand, within CA265_RS24600 at 5996.930 kb on - strand, within CA265_RS24600 at 5996.968 kb on + strand, within CA265_RS24600 at 5996.969 kb on - strand, within CA265_RS24600 at 5997.066 kb on + strand, within CA265_RS24600 at 5997.079 kb on - strand, within CA265_RS24600 at 5997.277 kb on - strand, within CA265_RS24600 at 5997.307 kb on + strand, within CA265_RS24600 at 5997.357 kb on + strand, within CA265_RS24600 at 5997.362 kb on - strand, within CA265_RS24600 at 5997.408 kb on - strand, within CA265_RS24600 at 5997.435 kb on - strand, within CA265_RS24600 at 5997.447 kb on - strand, within CA265_RS24600 at 5997.478 kb on + strand, within CA265_RS24600 at 5997.530 kb on + strand at 5997.530 kb on + strand, within CA265_RS24600 at 5997.573 kb on + strand, within CA265_RS24600 at 5997.575 kb on + strand, within CA265_RS24600 at 5997.588 kb on + strand, within CA265_RS24600 at 5997.608 kb on + strand, within CA265_RS24600 at 5997.618 kb on + strand, within CA265_RS24600 at 5997.619 kb on - strand, within CA265_RS24600 at 5997.628 kb on - strand, within CA265_RS24600 at 5997.628 kb on - strand, within CA265_RS24600 at 5997.655 kb on + strand, within CA265_RS24600 at 5997.656 kb on - strand, within CA265_RS24600 at 5997.762 kb on - strand, within CA265_RS24600 at 5997.762 kb on - strand, within CA265_RS24600 at 5997.771 kb on - strand, within CA265_RS24600 at 5997.771 kb on - strand at 5997.783 kb on + strand, within CA265_RS24600 at 5997.834 kb on - strand, within CA265_RS24600 at 5997.977 kb on + strand, within CA265_RS24600 at 5998.023 kb on + strand, within CA265_RS24600 at 5998.057 kb on - strand, within CA265_RS24600 at 5998.057 kb on - strand, within CA265_RS24600 at 5998.107 kb on + strand, within CA265_RS24600 at 5998.201 kb on + strand, within CA265_RS24600 at 5998.244 kb on + strand, within CA265_RS24600 at 5998.245 kb on - strand, within CA265_RS24600 at 5998.286 kb on + strand, within CA265_RS24600 at 5998.287 kb on - strand, within CA265_RS24600 at 5998.289 kb on - strand, within CA265_RS24600 at 5998.311 kb on + strand, within CA265_RS24600 at 5998.359 kb on - strand, within CA265_RS24600 at 5998.378 kb on + strand, within CA265_RS24600 at 5998.382 kb on + strand, within CA265_RS24600 at 5998.383 kb on - strand, within CA265_RS24600 at 5998.387 kb on + strand, within CA265_RS24600 at 5998.388 kb on - strand, within CA265_RS24600 at 5998.395 kb on + strand at 5998.445 kb on - strand at 5998.588 kb on - strand at 5998.588 kb on - strand at 5998.588 kb on - strand at 5998.601 kb on + strand at 5998.602 kb on - strand at 5998.676 kb on + strand at 5998.681 kb on - strand at 5998.731 kb on + strand at 5998.807 kb on - strand at 5999.004 kb on + strand at 5999.004 kb on + strand at 5999.005 kb on - strand at 5999.005 kb on - strand at 5999.005 kb on - strand at 5999.008 kb on + strand at 5999.009 kb on - strand at 5999.025 kb on - strand at 5999.063 kb on + strand at 5999.238 kb on + strand, within CA265_RS24605 at 5999.239 kb on - strand, within CA265_RS24605 at 5999.345 kb on + strand at 5999.346 kb on - strand at 5999.365 kb on + strand at 5999.366 kb on - strand at 5999.414 kb on - strand at 5999.419 kb on - strand at 5999.432 kb on - strand at 5999.493 kb on - strand at 5999.508 kb on + strand at 5999.508 kb on + strand at 5999.511 kb on + strand at 5999.512 kb on - strand at 5999.581 kb on + strand, within CA265_RS24610 at 5999.581 kb on + strand, within CA265_RS24610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 5,995,169 - -1.4 5,995,308 - CA265_RS24595 0.23 -0.9 5,995,314 + CA265_RS24595 0.24 +0.5 5,995,324 + CA265_RS24595 0.25 -0.3 5,995,349 + CA265_RS24595 0.28 +0.4 5,995,350 - CA265_RS24595 0.28 +0.4 5,995,376 + CA265_RS24595 0.31 -0.2 5,995,380 + CA265_RS24595 0.32 +0.2 5,995,381 - CA265_RS24595 0.32 -0.6 5,995,381 - CA265_RS24595 0.32 +0.1 5,995,399 - CA265_RS24595 0.34 -0.9 5,995,400 + CA265_RS24595 0.34 +0.9 5,995,507 - CA265_RS24595 0.47 +0.0 5,995,507 - CA265_RS24595 0.47 -0.4 5,995,638 - CA265_RS24595 0.62 +0.2 5,995,650 - CA265_RS24595 0.63 +0.0 5,995,661 - CA265_RS24595 0.65 +0.1 5,995,665 - CA265_RS24595 0.65 +0.4 5,995,679 - CA265_RS24595 0.67 -0.2 5,995,679 - CA265_RS24595 0.67 +0.1 5,995,686 + CA265_RS24595 0.68 +1.5 5,995,686 + CA265_RS24595 0.68 -1.2 5,995,686 + CA265_RS24595 0.68 -0.2 5,995,687 - CA265_RS24595 0.68 -0.4 5,995,768 - CA265_RS24595 0.77 -0.4 5,995,782 - CA265_RS24595 0.79 -0.7 5,995,782 - CA265_RS24595 0.79 -0.2 5,995,869 + CA265_RS24595 0.89 -0.1 5,995,893 - -0.6 5,995,956 + +0.6 5,995,956 + +1.2 5,995,957 - -0.8 5,995,957 - +1.0 5,995,970 + +1.1 5,995,989 - +0.1 5,995,989 - +0.2 5,996,090 + +0.7 5,996,134 + +0.8 5,996,164 + +0.7 5,996,164 + -0.2 5,996,165 - +0.1 5,996,199 + -0.0 5,996,270 - +0.7 5,996,279 - +0.8 5,996,279 - +1.4 5,996,279 - +0.5 5,996,282 + +0.1 5,996,420 + CA265_RS24600 0.10 -0.9 5,996,420 + CA265_RS24600 0.10 +1.0 5,996,422 + CA265_RS24600 0.10 +0.5 5,996,433 + CA265_RS24600 0.11 +1.3 5,996,433 + CA265_RS24600 0.11 -0.9 5,996,456 + CA265_RS24600 0.12 +0.4 5,996,456 + CA265_RS24600 0.12 +0.1 5,996,457 - CA265_RS24600 0.12 +0.4 5,996,457 - CA265_RS24600 0.12 -0.0 5,996,510 + CA265_RS24600 0.14 +0.5 5,996,511 - CA265_RS24600 0.14 +0.3 5,996,548 - CA265_RS24600 0.15 +0.2 5,996,614 + CA265_RS24600 0.18 -0.1 5,996,614 + CA265_RS24600 0.18 -0.5 5,996,615 - CA265_RS24600 0.18 -0.0 5,996,617 + CA265_RS24600 0.18 +1.1 5,996,618 - CA265_RS24600 0.18 -0.6 5,996,671 + CA265_RS24600 0.20 +0.7 5,996,674 + CA265_RS24600 0.21 +1.7 5,996,728 + CA265_RS24600 0.23 -0.2 5,996,738 + CA265_RS24600 0.23 +1.0 5,996,739 - CA265_RS24600 0.23 -1.4 5,996,739 - CA265_RS24600 0.23 -0.8 5,996,791 - CA265_RS24600 0.25 -0.6 5,996,805 + CA265_RS24600 0.26 +0.1 5,996,806 - CA265_RS24600 0.26 +0.2 5,996,806 - CA265_RS24600 0.26 +1.1 5,996,848 - CA265_RS24600 0.28 -2.4 5,996,905 + CA265_RS24600 0.30 +0.5 5,996,906 - CA265_RS24600 0.30 +1.2 5,996,908 + CA265_RS24600 0.30 -1.0 5,996,927 + CA265_RS24600 0.31 -0.5 5,996,928 - CA265_RS24600 0.31 +0.1 5,996,930 - CA265_RS24600 0.31 -0.2 5,996,968 + CA265_RS24600 0.32 +1.8 5,996,969 - CA265_RS24600 0.32 +0.2 5,997,066 + CA265_RS24600 0.36 +0.2 5,997,079 - CA265_RS24600 0.37 +0.8 5,997,277 - CA265_RS24600 0.45 +0.2 5,997,307 + CA265_RS24600 0.46 +1.4 5,997,357 + CA265_RS24600 0.48 +1.0 5,997,362 - CA265_RS24600 0.48 -0.3 5,997,408 - CA265_RS24600 0.50 +0.3 5,997,435 - CA265_RS24600 0.51 -0.0 5,997,447 - CA265_RS24600 0.52 -1.2 5,997,478 + CA265_RS24600 0.53 +0.3 5,997,530 + -0.2 5,997,530 + CA265_RS24600 0.55 +0.8 5,997,573 + CA265_RS24600 0.57 +1.4 5,997,575 + CA265_RS24600 0.57 +0.6 5,997,588 + CA265_RS24600 0.57 +0.1 5,997,608 + CA265_RS24600 0.58 +1.1 5,997,618 + CA265_RS24600 0.59 +1.4 5,997,619 - CA265_RS24600 0.59 -0.7 5,997,628 - CA265_RS24600 0.59 -0.2 5,997,628 - CA265_RS24600 0.59 -0.3 5,997,655 + CA265_RS24600 0.60 +1.2 5,997,656 - CA265_RS24600 0.60 +0.1 5,997,762 - CA265_RS24600 0.65 -0.6 5,997,762 - CA265_RS24600 0.65 -0.0 5,997,771 - CA265_RS24600 0.65 -0.6 5,997,771 - +0.8 5,997,783 + CA265_RS24600 0.65 +0.7 5,997,834 - CA265_RS24600 0.67 +0.1 5,997,977 + CA265_RS24600 0.73 +1.0 5,998,023 + CA265_RS24600 0.75 +0.8 5,998,057 - CA265_RS24600 0.76 +0.7 5,998,057 - CA265_RS24600 0.76 -0.1 5,998,107 + CA265_RS24600 0.78 +1.5 5,998,201 + CA265_RS24600 0.82 +2.0 5,998,244 + CA265_RS24600 0.84 +1.0 5,998,245 - CA265_RS24600 0.84 -1.0 5,998,286 + CA265_RS24600 0.86 +0.8 5,998,287 - CA265_RS24600 0.86 +0.8 5,998,289 - CA265_RS24600 0.86 +0.3 5,998,311 + CA265_RS24600 0.87 +1.0 5,998,359 - CA265_RS24600 0.89 +1.1 5,998,378 + CA265_RS24600 0.89 +1.4 5,998,382 + CA265_RS24600 0.90 +0.7 5,998,383 - CA265_RS24600 0.90 -0.3 5,998,387 + CA265_RS24600 0.90 +1.1 5,998,388 - CA265_RS24600 0.90 +0.2 5,998,395 + +1.3 5,998,445 - -1.5 5,998,588 - +1.2 5,998,588 - -0.5 5,998,588 - +0.5 5,998,601 + +0.4 5,998,602 - +0.5 5,998,676 + +1.8 5,998,681 - -0.2 5,998,731 + -0.8 5,998,807 - -0.5 5,999,004 + +0.1 5,999,004 + -3.1 5,999,005 - +1.2 5,999,005 - +0.4 5,999,005 - +1.7 5,999,008 + +0.8 5,999,009 - +0.6 5,999,025 - -0.6 5,999,063 + -0.5 5,999,238 + CA265_RS24605 0.80 -0.0 5,999,239 - CA265_RS24605 0.80 -0.0 5,999,345 + -0.3 5,999,346 - -0.6 5,999,365 + -0.7 5,999,366 - +1.8 5,999,414 - -0.8 5,999,419 - +1.2 5,999,432 - -0.4 5,999,493 - -0.1 5,999,508 + +0.2 5,999,508 + -0.2 5,999,511 + -0.9 5,999,512 - +0.5 5,999,581 + CA265_RS24610 0.13 -0.2 5,999,581 + CA265_RS24610 0.13 +1.5
Or see this region's nucleotide sequence