Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23270 and CA265_RS23275 are separated by 78 nucleotides CA265_RS23275 and CA265_RS23280 are separated by 101 nucleotides CA265_RS23280 and CA265_RS23285 are separated by 228 nucleotides
CA265_RS23270: CA265_RS23270 - hypothetical protein, at 5,687,079 to 5,687,762
_RS23270
CA265_RS23275: CA265_RS23275 - alpha/beta hydrolase, at 5,687,841 to 5,688,623
_RS23275
CA265_RS23280: CA265_RS23280 - sugar kinase, at 5,688,725 to 5,689,978
_RS23280
CA265_RS23285: CA265_RS23285 - oxidoreductase, at 5,690,207 to 5,691,493
_RS23285
Position (kb)
5688
5689
5690 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5687.732 kb on - strand at 5687.774 kb on - strand at 5687.824 kb on + strand at 5687.898 kb on + strand at 5687.899 kb on - strand at 5688.180 kb on - strand, within CA265_RS23275 at 5688.296 kb on + strand, within CA265_RS23275 at 5688.297 kb on - strand, within CA265_RS23275 at 5688.324 kb on - strand, within CA265_RS23275 at 5688.324 kb on - strand, within CA265_RS23275 at 5688.485 kb on + strand, within CA265_RS23275 at 5688.486 kb on - strand, within CA265_RS23275 at 5688.535 kb on - strand, within CA265_RS23275 at 5688.571 kb on + strand at 5688.572 kb on - strand at 5688.572 kb on - strand at 5688.572 kb on - strand at 5688.611 kb on + strand at 5688.661 kb on - strand at 5688.661 kb on - strand at 5688.675 kb on + strand at 5688.675 kb on + strand at 5688.778 kb on + strand at 5688.833 kb on + strand at 5688.833 kb on + strand at 5688.834 kb on - strand at 5688.867 kb on + strand, within CA265_RS23280 at 5688.901 kb on + strand, within CA265_RS23280 at 5688.901 kb on + strand, within CA265_RS23280 at 5688.901 kb on + strand, within CA265_RS23280 at 5688.902 kb on - strand, within CA265_RS23280 at 5688.903 kb on + strand, within CA265_RS23280 at 5688.956 kb on + strand, within CA265_RS23280 at 5688.957 kb on - strand, within CA265_RS23280 at 5689.100 kb on + strand, within CA265_RS23280 at 5689.101 kb on - strand, within CA265_RS23280 at 5689.101 kb on - strand, within CA265_RS23280 at 5689.125 kb on - strand, within CA265_RS23280 at 5689.285 kb on + strand, within CA265_RS23280 at 5689.286 kb on - strand, within CA265_RS23280 at 5689.286 kb on - strand, within CA265_RS23280 at 5689.314 kb on - strand, within CA265_RS23280 at 5689.319 kb on + strand, within CA265_RS23280 at 5689.401 kb on - strand, within CA265_RS23280 at 5689.474 kb on - strand, within CA265_RS23280 at 5689.510 kb on + strand, within CA265_RS23280 at 5689.519 kb on + strand, within CA265_RS23280 at 5689.520 kb on - strand, within CA265_RS23280 at 5689.520 kb on - strand, within CA265_RS23280 at 5689.564 kb on + strand, within CA265_RS23280 at 5689.584 kb on - strand, within CA265_RS23280 at 5689.584 kb on - strand, within CA265_RS23280 at 5689.657 kb on - strand, within CA265_RS23280 at 5689.676 kb on + strand, within CA265_RS23280 at 5689.743 kb on - strand, within CA265_RS23280 at 5689.829 kb on + strand, within CA265_RS23280 at 5689.841 kb on - strand, within CA265_RS23280 at 5689.900 kb on - strand at 5689.952 kb on + strand at 5689.962 kb on - strand at 5690.008 kb on - strand at 5690.098 kb on + strand at 5690.098 kb on + strand at 5690.106 kb on + strand at 5690.176 kb on + strand at 5690.306 kb on + strand at 5690.306 kb on + strand at 5690.307 kb on - strand at 5690.307 kb on - strand at 5690.419 kb on - strand, within CA265_RS23285 at 5690.424 kb on + strand, within CA265_RS23285 at 5690.424 kb on + strand, within CA265_RS23285 at 5690.424 kb on + strand, within CA265_RS23285 at 5690.425 kb on - strand, within CA265_RS23285 at 5690.449 kb on + strand, within CA265_RS23285 at 5690.470 kb on - strand, within CA265_RS23285 at 5690.473 kb on + strand, within CA265_RS23285 at 5690.500 kb on + strand, within CA265_RS23285 at 5690.510 kb on - strand, within CA265_RS23285 at 5690.510 kb on - strand, within CA265_RS23285 at 5690.511 kb on + strand, within CA265_RS23285 at 5690.512 kb on - strand, within CA265_RS23285 at 5690.566 kb on + strand, within CA265_RS23285 at 5690.566 kb on + strand, within CA265_RS23285 at 5690.567 kb on - strand, within CA265_RS23285 at 5690.567 kb on - strand, within CA265_RS23285 at 5690.569 kb on - strand, within CA265_RS23285 at 5690.577 kb on + strand, within CA265_RS23285 at 5690.578 kb on - strand, within CA265_RS23285 at 5690.578 kb on - strand, within CA265_RS23285 at 5690.578 kb on - strand, within CA265_RS23285 at 5690.603 kb on - strand, within CA265_RS23285 at 5690.630 kb on - strand, within CA265_RS23285 at 5690.634 kb on + strand, within CA265_RS23285 at 5690.677 kb on + strand, within CA265_RS23285 at 5690.678 kb on - strand, within CA265_RS23285 at 5690.678 kb on - strand, within CA265_RS23285 at 5690.678 kb on - strand, within CA265_RS23285 at 5690.705 kb on - strand, within CA265_RS23285 at 5690.710 kb on + strand, within CA265_RS23285 at 5690.734 kb on + strand, within CA265_RS23285 at 5690.735 kb on - strand, within CA265_RS23285 at 5690.802 kb on + strand, within CA265_RS23285 at 5690.802 kb on + strand, within CA265_RS23285 at 5690.802 kb on + strand, within CA265_RS23285 at 5690.802 kb on + strand, within CA265_RS23285 at 5690.803 kb on - strand, within CA265_RS23285 at 5690.888 kb on + strand, within CA265_RS23285 at 5690.890 kb on + strand, within CA265_RS23285 at 5690.891 kb on - strand, within CA265_RS23285 at 5690.917 kb on + strand, within CA265_RS23285 at 5690.924 kb on + strand, within CA265_RS23285 at 5690.925 kb on - strand, within CA265_RS23285 at 5690.925 kb on - strand, within CA265_RS23285 at 5690.970 kb on - strand, within CA265_RS23285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 5,687,732 - -0.2 5,687,774 - +0.1 5,687,824 + +0.3 5,687,898 + +0.4 5,687,899 - -0.1 5,688,180 - CA265_RS23275 0.43 -0.4 5,688,296 + CA265_RS23275 0.58 +0.4 5,688,297 - CA265_RS23275 0.58 -0.3 5,688,324 - CA265_RS23275 0.62 -0.6 5,688,324 - CA265_RS23275 0.62 -0.4 5,688,485 + CA265_RS23275 0.82 -0.1 5,688,486 - CA265_RS23275 0.82 -0.1 5,688,535 - CA265_RS23275 0.89 +1.1 5,688,571 + -0.8 5,688,572 - +0.1 5,688,572 - +0.4 5,688,572 - +0.6 5,688,611 + -0.1 5,688,661 - +1.5 5,688,661 - -0.1 5,688,675 + -0.4 5,688,675 + +0.1 5,688,778 + +0.3 5,688,833 + +0.8 5,688,833 + -0.3 5,688,834 - -1.2 5,688,867 + CA265_RS23280 0.11 +0.4 5,688,901 + CA265_RS23280 0.14 -0.0 5,688,901 + CA265_RS23280 0.14 -0.1 5,688,901 + CA265_RS23280 0.14 -0.5 5,688,902 - CA265_RS23280 0.14 -0.3 5,688,903 + CA265_RS23280 0.14 -0.4 5,688,956 + CA265_RS23280 0.18 -0.2 5,688,957 - CA265_RS23280 0.19 +0.3 5,689,100 + CA265_RS23280 0.30 -0.3 5,689,101 - CA265_RS23280 0.30 +0.4 5,689,101 - CA265_RS23280 0.30 -0.4 5,689,125 - CA265_RS23280 0.32 +0.2 5,689,285 + CA265_RS23280 0.45 -0.7 5,689,286 - CA265_RS23280 0.45 +0.1 5,689,286 - CA265_RS23280 0.45 -0.3 5,689,314 - CA265_RS23280 0.47 -0.0 5,689,319 + CA265_RS23280 0.47 -0.8 5,689,401 - CA265_RS23280 0.54 -0.7 5,689,474 - CA265_RS23280 0.60 -1.8 5,689,510 + CA265_RS23280 0.63 +0.0 5,689,519 + CA265_RS23280 0.63 +0.6 5,689,520 - CA265_RS23280 0.63 +0.2 5,689,520 - CA265_RS23280 0.63 +0.1 5,689,564 + CA265_RS23280 0.67 +0.7 5,689,584 - CA265_RS23280 0.69 +0.5 5,689,584 - CA265_RS23280 0.69 +0.5 5,689,657 - CA265_RS23280 0.74 -0.1 5,689,676 + CA265_RS23280 0.76 +1.4 5,689,743 - CA265_RS23280 0.81 +0.7 5,689,829 + CA265_RS23280 0.88 -0.1 5,689,841 - CA265_RS23280 0.89 -0.3 5,689,900 - +0.3 5,689,952 + -1.1 5,689,962 - -1.1 5,690,008 - -0.5 5,690,098 + -1.0 5,690,098 + -0.4 5,690,106 + -0.0 5,690,176 + +0.3 5,690,306 + +0.1 5,690,306 + +0.2 5,690,307 - -0.1 5,690,307 - +0.7 5,690,419 - CA265_RS23285 0.16 +2.1 5,690,424 + CA265_RS23285 0.17 -0.7 5,690,424 + CA265_RS23285 0.17 -1.1 5,690,424 + CA265_RS23285 0.17 -0.6 5,690,425 - CA265_RS23285 0.17 +2.2 5,690,449 + CA265_RS23285 0.19 -0.5 5,690,470 - CA265_RS23285 0.20 +1.0 5,690,473 + CA265_RS23285 0.21 +0.1 5,690,500 + CA265_RS23285 0.23 +0.2 5,690,510 - CA265_RS23285 0.24 +1.0 5,690,510 - CA265_RS23285 0.24 -0.9 5,690,511 + CA265_RS23285 0.24 -0.2 5,690,512 - CA265_RS23285 0.24 +0.1 5,690,566 + CA265_RS23285 0.28 -0.4 5,690,566 + CA265_RS23285 0.28 +0.3 5,690,567 - CA265_RS23285 0.28 -0.5 5,690,567 - CA265_RS23285 0.28 -0.6 5,690,569 - CA265_RS23285 0.28 +0.9 5,690,577 + CA265_RS23285 0.29 +0.6 5,690,578 - CA265_RS23285 0.29 +0.5 5,690,578 - CA265_RS23285 0.29 +1.2 5,690,578 - CA265_RS23285 0.29 +0.3 5,690,603 - CA265_RS23285 0.31 +0.4 5,690,630 - CA265_RS23285 0.33 -0.4 5,690,634 + CA265_RS23285 0.33 -1.4 5,690,677 + CA265_RS23285 0.37 -1.4 5,690,678 - CA265_RS23285 0.37 +1.2 5,690,678 - CA265_RS23285 0.37 +0.3 5,690,678 - CA265_RS23285 0.37 -0.3 5,690,705 - CA265_RS23285 0.39 +0.2 5,690,710 + CA265_RS23285 0.39 -0.3 5,690,734 + CA265_RS23285 0.41 +0.6 5,690,735 - CA265_RS23285 0.41 -1.5 5,690,802 + CA265_RS23285 0.46 -0.7 5,690,802 + CA265_RS23285 0.46 -0.3 5,690,802 + CA265_RS23285 0.46 -0.6 5,690,802 + CA265_RS23285 0.46 -0.2 5,690,803 - CA265_RS23285 0.46 -0.1 5,690,888 + CA265_RS23285 0.53 -0.7 5,690,890 + CA265_RS23285 0.53 +1.1 5,690,891 - CA265_RS23285 0.53 +0.2 5,690,917 + CA265_RS23285 0.55 +0.2 5,690,924 + CA265_RS23285 0.56 -0.1 5,690,925 - CA265_RS23285 0.56 +0.4 5,690,925 - CA265_RS23285 0.56 +1.2 5,690,970 - CA265_RS23285 0.59 -0.6
Or see this region's nucleotide sequence