Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21745 and CA265_RS21750 are separated by 460 nucleotides CA265_RS21750 and CA265_RS21755 are separated by 5 nucleotides CA265_RS21755 and CA265_RS21760 are separated by 4 nucleotides CA265_RS21760 and CA265_RS21765 are separated by 55 nucleotides CA265_RS21765 and CA265_RS21770 are separated by 234 nucleotides
CA265_RS21745: CA265_RS21745 - hypothetical protein, at 5,346,456 to 5,346,770
_RS21745
CA265_RS21750: CA265_RS21750 - hypothetical protein, at 5,347,231 to 5,347,497
_RS21750
CA265_RS21755: CA265_RS21755 - hypothetical protein, at 5,347,503 to 5,347,736
_RS21755
CA265_RS21760: CA265_RS21760 - hypothetical protein, at 5,347,741 to 5,348,133
_RS21760
CA265_RS21765: CA265_RS21765 - hypothetical protein, at 5,348,189 to 5,348,641
_RS21765
CA265_RS21770: CA265_RS21770 - ATP-dependent RNA helicase, at 5,348,876 to 5,350,048
_RS21770
Position (kb) 5347
5348
5349 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5346.893 kb on - strand at 5346.893 kb on - strand at 5347.009 kb on + strand at 5347.009 kb on + strand at 5347.010 kb on - strand at 5347.010 kb on - strand at 5347.010 kb on - strand at 5347.013 kb on + strand at 5347.019 kb on - strand at 5347.155 kb on - strand at 5347.208 kb on - strand at 5347.268 kb on + strand, within CA265_RS21750 at 5347.311 kb on + strand, within CA265_RS21750 at 5347.372 kb on + strand, within CA265_RS21750 at 5347.409 kb on - strand, within CA265_RS21750 at 5347.410 kb on + strand, within CA265_RS21750 at 5347.479 kb on + strand at 5347.482 kb on + strand at 5347.482 kb on + strand at 5347.487 kb on + strand at 5347.487 kb on + strand at 5347.519 kb on + strand at 5347.520 kb on - strand at 5347.562 kb on - strand, within CA265_RS21755 at 5347.601 kb on - strand, within CA265_RS21755 at 5347.620 kb on + strand, within CA265_RS21755 at 5347.650 kb on - strand, within CA265_RS21755 at 5347.663 kb on - strand, within CA265_RS21755 at 5347.701 kb on - strand, within CA265_RS21755 at 5347.718 kb on - strand at 5347.741 kb on - strand at 5347.741 kb on - strand at 5347.741 kb on - strand at 5347.741 kb on - strand at 5347.761 kb on - strand at 5347.861 kb on - strand, within CA265_RS21760 at 5347.901 kb on - strand, within CA265_RS21760 at 5347.901 kb on - strand, within CA265_RS21760 at 5347.939 kb on - strand, within CA265_RS21760 at 5348.011 kb on - strand, within CA265_RS21760 at 5348.011 kb on - strand, within CA265_RS21760 at 5348.044 kb on + strand, within CA265_RS21760 at 5348.066 kb on - strand, within CA265_RS21760 at 5348.132 kb on - strand at 5348.132 kb on - strand at 5348.132 kb on - strand at 5348.132 kb on - strand at 5348.132 kb on - strand at 5348.164 kb on - strand at 5348.203 kb on + strand at 5348.290 kb on + strand, within CA265_RS21765 at 5348.291 kb on - strand, within CA265_RS21765 at 5348.299 kb on + strand, within CA265_RS21765 at 5348.337 kb on + strand, within CA265_RS21765 at 5348.337 kb on + strand, within CA265_RS21765 at 5348.338 kb on - strand, within CA265_RS21765 at 5348.410 kb on + strand, within CA265_RS21765 at 5348.424 kb on + strand, within CA265_RS21765 at 5348.424 kb on + strand, within CA265_RS21765 at 5348.425 kb on - strand, within CA265_RS21765 at 5348.425 kb on - strand, within CA265_RS21765 at 5348.425 kb on - strand, within CA265_RS21765 at 5348.490 kb on - strand, within CA265_RS21765 at 5348.491 kb on + strand, within CA265_RS21765 at 5348.492 kb on - strand, within CA265_RS21765 at 5348.584 kb on + strand, within CA265_RS21765 at 5348.653 kb on + strand at 5348.653 kb on + strand at 5348.722 kb on - strand at 5348.778 kb on + strand at 5348.797 kb on + strand at 5348.797 kb on + strand at 5348.836 kb on + strand at 5348.862 kb on + strand at 5348.938 kb on + strand at 5349.037 kb on + strand, within CA265_RS21770 at 5349.037 kb on + strand, within CA265_RS21770 at 5349.043 kb on + strand, within CA265_RS21770 at 5349.044 kb on - strand, within CA265_RS21770 at 5349.082 kb on + strand, within CA265_RS21770 at 5349.097 kb on - strand, within CA265_RS21770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 5,346,893 - +1.3 5,346,893 - +0.5 5,347,009 + -0.6 5,347,009 + -1.2 5,347,010 - -0.1 5,347,010 - +0.5 5,347,010 - +1.8 5,347,013 + -1.1 5,347,019 - +0.7 5,347,155 - +0.5 5,347,208 - -1.0 5,347,268 + CA265_RS21750 0.14 +0.2 5,347,311 + CA265_RS21750 0.30 -0.1 5,347,372 + CA265_RS21750 0.53 -1.4 5,347,409 - CA265_RS21750 0.67 +0.4 5,347,410 + CA265_RS21750 0.67 +0.2 5,347,479 + -3.3 5,347,482 + +0.7 5,347,482 + -0.8 5,347,487 + -0.7 5,347,487 + +0.2 5,347,519 + +0.5 5,347,520 - +0.4 5,347,562 - CA265_RS21755 0.25 +0.8 5,347,601 - CA265_RS21755 0.42 +0.3 5,347,620 + CA265_RS21755 0.50 -0.4 5,347,650 - CA265_RS21755 0.63 -0.9 5,347,663 - CA265_RS21755 0.68 -2.2 5,347,701 - CA265_RS21755 0.85 +0.5 5,347,718 - +0.2 5,347,741 - +0.5 5,347,741 - +0.2 5,347,741 - +0.5 5,347,741 - +0.5 5,347,761 - -0.1 5,347,861 - CA265_RS21760 0.31 +1.0 5,347,901 - CA265_RS21760 0.41 +0.5 5,347,901 - CA265_RS21760 0.41 -1.4 5,347,939 - CA265_RS21760 0.50 -0.3 5,348,011 - CA265_RS21760 0.69 +1.0 5,348,011 - CA265_RS21760 0.69 -0.9 5,348,044 + CA265_RS21760 0.77 -1.7 5,348,066 - CA265_RS21760 0.83 +0.2 5,348,132 - +0.2 5,348,132 - -0.8 5,348,132 - +0.0 5,348,132 - -0.0 5,348,132 - +0.5 5,348,164 - +0.1 5,348,203 + +0.4 5,348,290 + CA265_RS21765 0.22 +2.7 5,348,291 - CA265_RS21765 0.23 +0.3 5,348,299 + CA265_RS21765 0.24 +0.4 5,348,337 + CA265_RS21765 0.33 +1.0 5,348,337 + CA265_RS21765 0.33 +0.7 5,348,338 - CA265_RS21765 0.33 +0.8 5,348,410 + CA265_RS21765 0.49 +0.5 5,348,424 + CA265_RS21765 0.52 +0.6 5,348,424 + CA265_RS21765 0.52 +1.7 5,348,425 - CA265_RS21765 0.52 -0.0 5,348,425 - CA265_RS21765 0.52 -0.5 5,348,425 - CA265_RS21765 0.52 -0.8 5,348,490 - CA265_RS21765 0.66 +0.4 5,348,491 + CA265_RS21765 0.67 +0.9 5,348,492 - CA265_RS21765 0.67 -1.3 5,348,584 + CA265_RS21765 0.87 +1.0 5,348,653 + -2.6 5,348,653 + -0.2 5,348,722 - -2.2 5,348,778 + -0.8 5,348,797 + -0.6 5,348,797 + -0.9 5,348,836 + -0.3 5,348,862 + -0.5 5,348,938 + -1.1 5,349,037 + CA265_RS21770 0.14 +0.4 5,349,037 + CA265_RS21770 0.14 +0.7 5,349,043 + CA265_RS21770 0.14 +1.0 5,349,044 - CA265_RS21770 0.14 +0.4 5,349,082 + CA265_RS21770 0.18 +0.0 5,349,097 - CA265_RS21770 0.19 +0.1
Or see this region's nucleotide sequence