Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21665 and CA265_RS21670 are separated by 5 nucleotides CA265_RS21670 and CA265_RS21675 are separated by 237 nucleotides CA265_RS21675 and CA265_RS21680 are separated by 63 nucleotides
CA265_RS21665: CA265_RS21665 - hypothetical protein, at 5,329,811 to 5,331,598
_RS21665
CA265_RS21670: CA265_RS21670 - hypothetical protein, at 5,331,604 to 5,331,972
_RS21670
CA265_RS21675: CA265_RS21675 - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 5,332,210 to 5,333,625
_RS21675
CA265_RS21680: CA265_RS21680 - sigma-54-dependent Fis family transcriptional regulator, at 5,333,689 to 5,334,936
_RS21680
Position (kb)
5332
5333
5334 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5331.234 kb on - strand, within CA265_RS21665 at 5331.257 kb on + strand, within CA265_RS21665 at 5331.261 kb on - strand, within CA265_RS21665 at 5331.274 kb on + strand, within CA265_RS21665 at 5331.296 kb on - strand, within CA265_RS21665 at 5331.402 kb on - strand, within CA265_RS21665 at 5331.454 kb on + strand at 5331.455 kb on - strand at 5331.455 kb on - strand at 5331.455 kb on - strand at 5331.456 kb on + strand at 5331.456 kb on + strand at 5331.457 kb on - strand at 5331.457 kb on - strand at 5331.488 kb on - strand at 5331.542 kb on + strand at 5331.542 kb on + strand at 5331.543 kb on - strand at 5331.551 kb on + strand at 5331.552 kb on - strand at 5331.552 kb on - strand at 5331.583 kb on + strand at 5331.583 kb on + strand at 5331.600 kb on + strand at 5331.601 kb on - strand at 5331.620 kb on + strand at 5331.621 kb on - strand at 5331.621 kb on - strand at 5331.627 kb on + strand at 5331.628 kb on - strand at 5331.771 kb on + strand, within CA265_RS21670 at 5331.812 kb on - strand, within CA265_RS21670 at 5331.818 kb on - strand at 5331.819 kb on + strand, within CA265_RS21670 at 5331.874 kb on + strand, within CA265_RS21670 at 5331.875 kb on - strand, within CA265_RS21670 at 5331.910 kb on - strand, within CA265_RS21670 at 5331.962 kb on - strand at 5331.997 kb on - strand at 5331.997 kb on - strand at 5332.052 kb on - strand at 5332.085 kb on + strand at 5332.086 kb on - strand at 5332.117 kb on + strand at 5332.204 kb on + strand at 5332.226 kb on + strand at 5332.309 kb on + strand at 5332.467 kb on + strand, within CA265_RS21675 at 5332.517 kb on + strand, within CA265_RS21675 at 5332.583 kb on + strand, within CA265_RS21675 at 5332.592 kb on + strand, within CA265_RS21675 at 5332.592 kb on + strand, within CA265_RS21675 at 5332.670 kb on + strand, within CA265_RS21675 at 5332.676 kb on + strand, within CA265_RS21675 at 5332.676 kb on + strand, within CA265_RS21675 at 5332.701 kb on + strand, within CA265_RS21675 at 5332.764 kb on + strand, within CA265_RS21675 at 5332.764 kb on + strand, within CA265_RS21675 at 5332.881 kb on + strand, within CA265_RS21675 at 5332.980 kb on + strand, within CA265_RS21675 at 5333.049 kb on + strand, within CA265_RS21675 at 5333.049 kb on + strand, within CA265_RS21675 at 5333.049 kb on + strand, within CA265_RS21675 at 5333.049 kb on + strand, within CA265_RS21675 at 5333.073 kb on + strand, within CA265_RS21675 at 5333.073 kb on + strand, within CA265_RS21675 at 5333.114 kb on + strand, within CA265_RS21675 at 5333.154 kb on + strand, within CA265_RS21675 at 5333.275 kb on + strand, within CA265_RS21675 at 5333.296 kb on + strand, within CA265_RS21675 at 5333.298 kb on + strand, within CA265_RS21675 at 5333.472 kb on + strand, within CA265_RS21675 at 5333.514 kb on + strand at 5333.641 kb on + strand at 5333.655 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 5,331,234 - CA265_RS21665 0.80 -0.8 5,331,257 + CA265_RS21665 0.81 +0.9 5,331,261 - CA265_RS21665 0.81 -0.2 5,331,274 + CA265_RS21665 0.82 +2.3 5,331,296 - CA265_RS21665 0.83 -1.2 5,331,402 - CA265_RS21665 0.89 -0.9 5,331,454 + +0.8 5,331,455 - -1.6 5,331,455 - -1.0 5,331,455 - -0.6 5,331,456 + +0.9 5,331,456 + +1.5 5,331,457 - -1.4 5,331,457 - -0.4 5,331,488 - -1.1 5,331,542 + +1.6 5,331,542 + +2.1 5,331,543 - -0.1 5,331,551 + +2.8 5,331,552 - +0.2 5,331,552 - +0.1 5,331,583 + +1.9 5,331,583 + +2.5 5,331,600 + +1.2 5,331,601 - +0.3 5,331,620 + +0.5 5,331,621 - -0.8 5,331,621 - -0.1 5,331,627 + +2.2 5,331,628 - -1.0 5,331,771 + CA265_RS21670 0.45 +2.5 5,331,812 - CA265_RS21670 0.56 -1.8 5,331,818 - -1.1 5,331,819 + CA265_RS21670 0.58 +1.2 5,331,874 + CA265_RS21670 0.73 +1.5 5,331,875 - CA265_RS21670 0.73 -0.9 5,331,910 - CA265_RS21670 0.83 +0.1 5,331,962 - -1.3 5,331,997 - -1.5 5,331,997 - -0.6 5,332,052 - -0.3 5,332,085 + +2.0 5,332,086 - -1.4 5,332,117 + +2.1 5,332,204 + +0.9 5,332,226 + -0.4 5,332,309 + +0.4 5,332,467 + CA265_RS21675 0.18 -1.1 5,332,517 + CA265_RS21675 0.22 +1.8 5,332,583 + CA265_RS21675 0.26 +1.2 5,332,592 + CA265_RS21675 0.27 +0.5 5,332,592 + CA265_RS21675 0.27 -0.1 5,332,670 + CA265_RS21675 0.32 +0.6 5,332,676 + CA265_RS21675 0.33 +0.5 5,332,676 + CA265_RS21675 0.33 +0.3 5,332,701 + CA265_RS21675 0.35 -1.7 5,332,764 + CA265_RS21675 0.39 -0.4 5,332,764 + CA265_RS21675 0.39 +1.2 5,332,881 + CA265_RS21675 0.47 -0.2 5,332,980 + CA265_RS21675 0.54 +1.3 5,333,049 + CA265_RS21675 0.59 +1.6 5,333,049 + CA265_RS21675 0.59 +1.1 5,333,049 + CA265_RS21675 0.59 +1.0 5,333,049 + CA265_RS21675 0.59 +1.1 5,333,073 + CA265_RS21675 0.61 +0.6 5,333,073 + CA265_RS21675 0.61 +1.4 5,333,114 + CA265_RS21675 0.64 +0.2 5,333,154 + CA265_RS21675 0.67 +1.3 5,333,275 + CA265_RS21675 0.75 +0.1 5,333,296 + CA265_RS21675 0.77 +0.5 5,333,298 + CA265_RS21675 0.77 +1.0 5,333,472 + CA265_RS21675 0.89 +1.1 5,333,514 + +1.3 5,333,641 + +1.4 5,333,655 + +1.4
Or see this region's nucleotide sequence