Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21025 and CA265_RS21030 overlap by 1 nucleotides CA265_RS21030 and CA265_RS21035 are separated by 78 nucleotides CA265_RS21035 and CA265_RS21040 overlap by 4 nucleotides
CA265_RS21025: CA265_RS21025 - histidine kinase, at 5,178,878 to 5,180,755
_RS21025
CA265_RS21030: CA265_RS21030 - hypothetical protein, at 5,180,755 to 5,181,408
_RS21030
CA265_RS21035: CA265_RS21035 - 3-oxoacyl-ACP synthase, at 5,181,487 to 5,182,563
_RS21035
CA265_RS21040: CA265_RS21040 - hypothetical protein, at 5,182,560 to 5,184,047
_RS21040
Position (kb) 5181
5182
5183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5180.497 kb on - strand, within CA265_RS21025 at 5180.527 kb on + strand, within CA265_RS21025 at 5180.528 kb on - strand, within CA265_RS21025 at 5180.533 kb on + strand, within CA265_RS21025 at 5180.563 kb on - strand, within CA265_RS21025 at 5180.623 kb on + strand at 5180.710 kb on + strand at 5180.753 kb on + strand at 5180.805 kb on + strand at 5181.017 kb on + strand, within CA265_RS21030 at 5181.026 kb on - strand, within CA265_RS21030 at 5181.126 kb on + strand, within CA265_RS21030 at 5181.128 kb on + strand, within CA265_RS21030 at 5181.128 kb on + strand, within CA265_RS21030 at 5181.129 kb on - strand, within CA265_RS21030 at 5181.189 kb on + strand, within CA265_RS21030 at 5181.255 kb on - strand, within CA265_RS21030 at 5181.255 kb on - strand, within CA265_RS21030 at 5181.301 kb on - strand, within CA265_RS21030 at 5181.309 kb on - strand, within CA265_RS21030 at 5181.322 kb on - strand, within CA265_RS21030 at 5181.351 kb on - strand at 5181.437 kb on + strand at 5181.437 kb on + strand at 5181.438 kb on - strand at 5181.438 kb on - strand at 5181.438 kb on - strand at 5181.483 kb on - strand at 5181.504 kb on + strand at 5181.505 kb on - strand at 5181.530 kb on - strand at 5181.547 kb on - strand at 5181.664 kb on + strand, within CA265_RS21035 at 5181.684 kb on - strand, within CA265_RS21035 at 5181.735 kb on + strand, within CA265_RS21035 at 5181.736 kb on - strand, within CA265_RS21035 at 5181.760 kb on - strand, within CA265_RS21035 at 5181.760 kb on - strand, within CA265_RS21035 at 5181.760 kb on - strand, within CA265_RS21035 at 5181.762 kb on + strand, within CA265_RS21035 at 5181.762 kb on + strand, within CA265_RS21035 at 5181.814 kb on - strand, within CA265_RS21035 at 5181.881 kb on - strand, within CA265_RS21035 at 5181.935 kb on - strand, within CA265_RS21035 at 5181.937 kb on + strand, within CA265_RS21035 at 5181.938 kb on - strand, within CA265_RS21035 at 5181.938 kb on - strand, within CA265_RS21035 at 5181.966 kb on + strand, within CA265_RS21035 at 5181.989 kb on + strand, within CA265_RS21035 at 5182.020 kb on + strand, within CA265_RS21035 at 5182.091 kb on + strand, within CA265_RS21035 at 5182.092 kb on - strand, within CA265_RS21035 at 5182.094 kb on + strand, within CA265_RS21035 at 5182.131 kb on + strand, within CA265_RS21035 at 5182.214 kb on - strand, within CA265_RS21035 at 5182.294 kb on - strand, within CA265_RS21035 at 5182.294 kb on - strand, within CA265_RS21035 at 5182.298 kb on + strand, within CA265_RS21035 at 5182.413 kb on - strand, within CA265_RS21035 at 5182.448 kb on + strand, within CA265_RS21035 at 5182.457 kb on + strand at 5182.520 kb on + strand at 5182.540 kb on - strand at 5182.540 kb on - strand at 5182.548 kb on + strand at 5182.643 kb on + strand at 5182.670 kb on + strand at 5182.743 kb on - strand, within CA265_RS21040 at 5182.804 kb on + strand, within CA265_RS21040 at 5182.805 kb on - strand, within CA265_RS21040 at 5182.805 kb on - strand, within CA265_RS21040 at 5182.838 kb on + strand, within CA265_RS21040 at 5182.855 kb on + strand, within CA265_RS21040 at 5182.855 kb on + strand, within CA265_RS21040 at 5182.856 kb on - strand, within CA265_RS21040 at 5182.882 kb on - strand, within CA265_RS21040 at 5182.900 kb on - strand, within CA265_RS21040 at 5182.996 kb on + strand, within CA265_RS21040 at 5182.996 kb on + strand, within CA265_RS21040 at 5182.996 kb on + strand, within CA265_RS21040 at 5182.996 kb on + strand, within CA265_RS21040 at 5182.997 kb on - strand, within CA265_RS21040 at 5182.997 kb on - strand, within CA265_RS21040 at 5183.000 kb on + strand, within CA265_RS21040 at 5183.000 kb on + strand, within CA265_RS21040 at 5183.001 kb on - strand, within CA265_RS21040 at 5183.086 kb on + strand, within CA265_RS21040 at 5183.087 kb on - strand, within CA265_RS21040 at 5183.196 kb on + strand, within CA265_RS21040 at 5183.197 kb on - strand, within CA265_RS21040 at 5183.215 kb on + strand, within CA265_RS21040 at 5183.277 kb on - strand, within CA265_RS21040 at 5183.287 kb on + strand, within CA265_RS21040 at 5183.287 kb on + strand, within CA265_RS21040 at 5183.287 kb on + strand, within CA265_RS21040 at 5183.288 kb on - strand, within CA265_RS21040 at 5183.338 kb on + strand, within CA265_RS21040 at 5183.338 kb on + strand, within CA265_RS21040 at 5183.338 kb on + strand, within CA265_RS21040 at 5183.339 kb on - strand, within CA265_RS21040 at 5183.339 kb on - strand, within CA265_RS21040 at 5183.370 kb on - strand, within CA265_RS21040 at 5183.370 kb on - strand, within CA265_RS21040 at 5183.388 kb on + strand, within CA265_RS21040 at 5183.392 kb on + strand, within CA265_RS21040 at 5183.392 kb on + strand, within CA265_RS21040 at 5183.392 kb on + strand, within CA265_RS21040 at 5183.472 kb on - strand, within CA265_RS21040 at 5183.475 kb on + strand, within CA265_RS21040 at 5183.514 kb on + strand, within CA265_RS21040 at 5183.515 kb on - strand, within CA265_RS21040 at 5183.526 kb on + strand, within CA265_RS21040 at 5183.561 kb on + strand, within CA265_RS21040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 5,180,497 - CA265_RS21025 0.86 +0.4 5,180,527 + CA265_RS21025 0.88 +0.4 5,180,528 - CA265_RS21025 0.88 +0.1 5,180,533 + CA265_RS21025 0.88 +0.2 5,180,563 - CA265_RS21025 0.90 -1.1 5,180,623 + -0.1 5,180,710 + +0.3 5,180,753 + +0.4 5,180,805 + -0.9 5,181,017 + CA265_RS21030 0.40 +1.4 5,181,026 - CA265_RS21030 0.41 +0.6 5,181,126 + CA265_RS21030 0.57 -1.4 5,181,128 + CA265_RS21030 0.57 +1.5 5,181,128 + CA265_RS21030 0.57 +1.1 5,181,129 - CA265_RS21030 0.57 -0.4 5,181,189 + CA265_RS21030 0.66 +0.6 5,181,255 - CA265_RS21030 0.76 -0.3 5,181,255 - CA265_RS21030 0.76 -0.2 5,181,301 - CA265_RS21030 0.83 +0.8 5,181,309 - CA265_RS21030 0.85 -1.1 5,181,322 - CA265_RS21030 0.87 -0.7 5,181,351 - -0.2 5,181,437 + -0.1 5,181,437 + +2.2 5,181,438 - -0.1 5,181,438 - -1.8 5,181,438 - -0.5 5,181,483 - -1.1 5,181,504 + -0.2 5,181,505 - +0.0 5,181,530 - +0.3 5,181,547 - -0.3 5,181,664 + CA265_RS21035 0.16 -0.1 5,181,684 - CA265_RS21035 0.18 -0.1 5,181,735 + CA265_RS21035 0.23 -0.7 5,181,736 - CA265_RS21035 0.23 -1.3 5,181,760 - CA265_RS21035 0.25 -0.7 5,181,760 - CA265_RS21035 0.25 -1.2 5,181,760 - CA265_RS21035 0.25 +0.0 5,181,762 + CA265_RS21035 0.26 +0.0 5,181,762 + CA265_RS21035 0.26 -1.1 5,181,814 - CA265_RS21035 0.30 +0.5 5,181,881 - CA265_RS21035 0.37 -0.7 5,181,935 - CA265_RS21035 0.42 -1.0 5,181,937 + CA265_RS21035 0.42 +1.1 5,181,938 - CA265_RS21035 0.42 +0.6 5,181,938 - CA265_RS21035 0.42 -0.3 5,181,966 + CA265_RS21035 0.44 -0.1 5,181,989 + CA265_RS21035 0.47 -0.2 5,182,020 + CA265_RS21035 0.49 +1.1 5,182,091 + CA265_RS21035 0.56 -0.6 5,182,092 - CA265_RS21035 0.56 -1.5 5,182,094 + CA265_RS21035 0.56 +0.2 5,182,131 + CA265_RS21035 0.60 +0.1 5,182,214 - CA265_RS21035 0.68 +0.3 5,182,294 - CA265_RS21035 0.75 -0.4 5,182,294 - CA265_RS21035 0.75 -0.3 5,182,298 + CA265_RS21035 0.75 -0.8 5,182,413 - CA265_RS21035 0.86 -1.0 5,182,448 + CA265_RS21035 0.89 -1.7 5,182,457 + -0.7 5,182,520 + +1.0 5,182,540 - -1.3 5,182,540 - +0.0 5,182,548 + -0.1 5,182,643 + -1.6 5,182,670 + -1.3 5,182,743 - CA265_RS21040 0.12 -0.9 5,182,804 + CA265_RS21040 0.16 -1.5 5,182,805 - CA265_RS21040 0.16 +0.1 5,182,805 - CA265_RS21040 0.16 -0.3 5,182,838 + CA265_RS21040 0.19 -0.1 5,182,855 + CA265_RS21040 0.20 +0.2 5,182,855 + CA265_RS21040 0.20 -1.1 5,182,856 - CA265_RS21040 0.20 -1.9 5,182,882 - CA265_RS21040 0.22 +0.7 5,182,900 - CA265_RS21040 0.23 -0.9 5,182,996 + CA265_RS21040 0.29 +1.9 5,182,996 + CA265_RS21040 0.29 +0.3 5,182,996 + CA265_RS21040 0.29 -0.8 5,182,996 + CA265_RS21040 0.29 -2.6 5,182,997 - CA265_RS21040 0.29 -0.7 5,182,997 - CA265_RS21040 0.29 -1.4 5,183,000 + CA265_RS21040 0.30 -1.6 5,183,000 + CA265_RS21040 0.30 -0.6 5,183,001 - CA265_RS21040 0.30 -1.3 5,183,086 + CA265_RS21040 0.35 +0.2 5,183,087 - CA265_RS21040 0.35 -0.1 5,183,196 + CA265_RS21040 0.43 -0.6 5,183,197 - CA265_RS21040 0.43 -0.2 5,183,215 + CA265_RS21040 0.44 -0.4 5,183,277 - CA265_RS21040 0.48 -1.1 5,183,287 + CA265_RS21040 0.49 -1.9 5,183,287 + CA265_RS21040 0.49 -0.1 5,183,287 + CA265_RS21040 0.49 +0.1 5,183,288 - CA265_RS21040 0.49 -2.2 5,183,338 + CA265_RS21040 0.52 -1.2 5,183,338 + CA265_RS21040 0.52 -1.2 5,183,338 + CA265_RS21040 0.52 -0.1 5,183,339 - CA265_RS21040 0.52 +1.5 5,183,339 - CA265_RS21040 0.52 -0.4 5,183,370 - CA265_RS21040 0.54 +0.1 5,183,370 - CA265_RS21040 0.54 +0.1 5,183,388 + CA265_RS21040 0.56 +0.8 5,183,392 + CA265_RS21040 0.56 +0.6 5,183,392 + CA265_RS21040 0.56 -1.1 5,183,392 + CA265_RS21040 0.56 -2.3 5,183,472 - CA265_RS21040 0.61 -0.2 5,183,475 + CA265_RS21040 0.61 -3.2 5,183,514 + CA265_RS21040 0.64 +0.5 5,183,515 - CA265_RS21040 0.64 +0.6 5,183,526 + CA265_RS21040 0.65 -0.8 5,183,561 + CA265_RS21040 0.67 -0.8
Or see this region's nucleotide sequence