Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19585 and CA265_RS19590 are separated by 185 nucleotides CA265_RS19590 and CA265_RS19595 are separated by 110 nucleotides CA265_RS19595 and CA265_RS19600 are separated by 201 nucleotides CA265_RS19600 and CA265_RS19605 are separated by 68 nucleotides
CA265_RS19585: CA265_RS19585 - pyruvate dehydrogenase, at 4,855,144 to 4,856,832
_RS19585
CA265_RS19590: CA265_RS19590 - hypothetical protein, at 4,857,018 to 4,857,296
_RS19590
CA265_RS19595: CA265_RS19595 - GxxExxY protein, at 4,857,407 to 4,857,784
_RS19595
CA265_RS19600: CA265_RS19600 - hypothetical protein, at 4,857,986 to 4,858,537
_RS19600
CA265_RS19605: CA265_RS19605 - serine hydrolase, at 4,858,606 to 4,859,838
_RS19605
Position (kb)
4857
4858 Strain fitness (log2 ratio)
-1
0
1
2 at 4856.825 kb on + strand at 4856.870 kb on + strand at 4856.875 kb on - strand at 4856.954 kb on + strand at 4857.036 kb on - strand at 4857.038 kb on + strand at 4857.044 kb on + strand at 4857.044 kb on + strand at 4857.100 kb on - strand, within CA265_RS19590 at 4857.110 kb on - strand, within CA265_RS19590 at 4857.125 kb on - strand, within CA265_RS19590 at 4857.270 kb on + strand at 4857.271 kb on - strand at 4857.272 kb on + strand at 4857.357 kb on + strand at 4857.358 kb on - strand at 4857.405 kb on + strand at 4857.502 kb on - strand, within CA265_RS19595 at 4857.505 kb on + strand, within CA265_RS19595 at 4857.506 kb on - strand, within CA265_RS19595 at 4857.524 kb on + strand, within CA265_RS19595 at 4857.542 kb on + strand, within CA265_RS19595 at 4857.563 kb on + strand, within CA265_RS19595 at 4857.575 kb on + strand, within CA265_RS19595 at 4857.578 kb on + strand, within CA265_RS19595 at 4857.601 kb on + strand, within CA265_RS19595 at 4857.606 kb on - strand, within CA265_RS19595 at 4857.612 kb on - strand, within CA265_RS19595 at 4857.613 kb on + strand, within CA265_RS19595 at 4857.632 kb on + strand, within CA265_RS19595 at 4857.678 kb on + strand, within CA265_RS19595 at 4857.723 kb on - strand, within CA265_RS19595 at 4857.728 kb on - strand, within CA265_RS19595 at 4857.795 kb on - strand at 4857.803 kb on - strand at 4857.825 kb on + strand at 4857.846 kb on - strand at 4857.886 kb on + strand at 4857.956 kb on + strand at 4858.093 kb on + strand, within CA265_RS19600 at 4858.158 kb on - strand, within CA265_RS19600 at 4858.160 kb on - strand, within CA265_RS19600 at 4858.160 kb on - strand, within CA265_RS19600 at 4858.160 kb on - strand, within CA265_RS19600 at 4858.173 kb on + strand, within CA265_RS19600 at 4858.180 kb on + strand, within CA265_RS19600 at 4858.199 kb on + strand, within CA265_RS19600 at 4858.231 kb on - strand, within CA265_RS19600 at 4858.252 kb on - strand, within CA265_RS19600 at 4858.252 kb on - strand, within CA265_RS19600 at 4858.279 kb on + strand, within CA265_RS19600 at 4858.280 kb on - strand, within CA265_RS19600 at 4858.323 kb on + strand, within CA265_RS19600 at 4858.324 kb on - strand, within CA265_RS19600 at 4858.386 kb on + strand, within CA265_RS19600 at 4858.391 kb on + strand, within CA265_RS19600 at 4858.398 kb on + strand, within CA265_RS19600 at 4858.399 kb on - strand, within CA265_RS19600 at 4858.498 kb on - strand at 4858.592 kb on + strand at 4858.602 kb on - strand at 4858.602 kb on - strand at 4858.620 kb on + strand at 4858.620 kb on + strand at 4858.620 kb on + strand at 4858.621 kb on - strand at 4858.622 kb on + strand at 4858.622 kb on + strand at 4858.753 kb on + strand, within CA265_RS19605
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 4,856,825 + -1.5 4,856,870 + -0.5 4,856,875 - +0.5 4,856,954 + +1.1 4,857,036 - +0.4 4,857,038 + +0.9 4,857,044 + +0.8 4,857,044 + +0.9 4,857,100 - CA265_RS19590 0.29 +0.7 4,857,110 - CA265_RS19590 0.33 +0.1 4,857,125 - CA265_RS19590 0.38 +0.9 4,857,270 + +0.9 4,857,271 - +1.7 4,857,272 + +0.7 4,857,357 + +0.3 4,857,358 - -0.8 4,857,405 + +1.3 4,857,502 - CA265_RS19595 0.25 +0.2 4,857,505 + CA265_RS19595 0.26 +0.5 4,857,506 - CA265_RS19595 0.26 +1.0 4,857,524 + CA265_RS19595 0.31 +0.5 4,857,542 + CA265_RS19595 0.36 -0.5 4,857,563 + CA265_RS19595 0.41 -0.3 4,857,575 + CA265_RS19595 0.44 +0.4 4,857,578 + CA265_RS19595 0.45 +0.5 4,857,601 + CA265_RS19595 0.51 +0.2 4,857,606 - CA265_RS19595 0.53 +0.6 4,857,612 - CA265_RS19595 0.54 +0.5 4,857,613 + CA265_RS19595 0.54 +0.9 4,857,632 + CA265_RS19595 0.60 +0.7 4,857,678 + CA265_RS19595 0.72 +0.5 4,857,723 - CA265_RS19595 0.84 +0.8 4,857,728 - CA265_RS19595 0.85 -0.9 4,857,795 - +1.0 4,857,803 - +0.3 4,857,825 + +1.3 4,857,846 - -1.1 4,857,886 + -0.1 4,857,956 + -0.4 4,858,093 + CA265_RS19600 0.19 +0.0 4,858,158 - CA265_RS19600 0.31 +0.2 4,858,160 - CA265_RS19600 0.32 -0.1 4,858,160 - CA265_RS19600 0.32 -1.6 4,858,160 - CA265_RS19600 0.32 +0.3 4,858,173 + CA265_RS19600 0.34 -1.0 4,858,180 + CA265_RS19600 0.35 -1.2 4,858,199 + CA265_RS19600 0.39 +1.0 4,858,231 - CA265_RS19600 0.44 +0.3 4,858,252 - CA265_RS19600 0.48 +0.6 4,858,252 - CA265_RS19600 0.48 -0.6 4,858,279 + CA265_RS19600 0.53 -0.9 4,858,280 - CA265_RS19600 0.53 -1.3 4,858,323 + CA265_RS19600 0.61 +1.0 4,858,324 - CA265_RS19600 0.61 +0.2 4,858,386 + CA265_RS19600 0.72 +1.0 4,858,391 + CA265_RS19600 0.73 +0.8 4,858,398 + CA265_RS19600 0.75 +0.5 4,858,399 - CA265_RS19600 0.75 -0.0 4,858,498 - +0.1 4,858,592 + +0.6 4,858,602 - +0.7 4,858,602 - +1.7 4,858,620 + +2.0 4,858,620 + +2.2 4,858,620 + +2.0 4,858,621 - +1.9 4,858,622 + +2.4 4,858,622 + +1.5 4,858,753 + CA265_RS19605 0.12 +2.0
Or see this region's nucleotide sequence